Micromechanical antibody sensor
Abstract
A sensor apparatus is provided using a microcantilevered spring element having a coating of a detector molecule such as an antibody or antigen. A sample containing a target molecule or substrate is provided to the coating. The spring element bends in response to the stress induced by the binding which occurs between the detector and target molecules. Deflections of the cantilever are detected by a variety of detection techniques. The microcantilever may be approximately 1 to 200 .mu.m long, approximately 1 to 50 .mu.m wide, and approximately 0.3 to 3.0 .mu.m thick. A sensitivity for detection of deflections is in the range of 0.01 nanometers.
- Inventors:
-
- Knoxville, TN
- Oak Ridge, TN
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- OSTI Identifier:
- 873996
- Patent Number(s):
- 6289717
- Assignee:
- U. T. Battelle, LLC (Oak Ridge, TN)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- AC05-96OR22464
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- micromechanical; antibody; sensor; apparatus; provided; microcantilevered; spring; element; coating; detector; molecule; antigen; sample; containing; target; substrate; bends; response; stress; induced; binding; occurs; molecules; deflections; cantilever; detected; variety; detection; techniques; microcantilever; approximately; 200; 50; wide; thick; sensitivity; range; 01; nanometers; detection techniques; spring element; sample containing; sensor apparatus; stress induced; microcantilevered spring; target molecule; /73/422/436/
Citation Formats
Thundat, Thomas G, Jacobson, K Bruce, Doktycz, Mitchel J, Kennel, Stephen J, and Warmack, Robert J. Micromechanical antibody sensor. United States: N. p., 2001.
Web.
Thundat, Thomas G, Jacobson, K Bruce, Doktycz, Mitchel J, Kennel, Stephen J, & Warmack, Robert J. Micromechanical antibody sensor. United States.
Thundat, Thomas G, Jacobson, K Bruce, Doktycz, Mitchel J, Kennel, Stephen J, and Warmack, Robert J. Mon .
"Micromechanical antibody sensor". United States. https://www.osti.gov/servlets/purl/873996.
@article{osti_873996,
title = {Micromechanical antibody sensor},
author = {Thundat, Thomas G and Jacobson, K Bruce and Doktycz, Mitchel J and Kennel, Stephen J and Warmack, Robert J},
abstractNote = {A sensor apparatus is provided using a microcantilevered spring element having a coating of a detector molecule such as an antibody or antigen. A sample containing a target molecule or substrate is provided to the coating. The spring element bends in response to the stress induced by the binding which occurs between the detector and target molecules. Deflections of the cantilever are detected by a variety of detection techniques. The microcantilever may be approximately 1 to 200 .mu.m long, approximately 1 to 50 .mu.m wide, and approximately 0.3 to 3.0 .mu.m thick. A sensitivity for detection of deflections is in the range of 0.01 nanometers.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2001},
month = {Mon Jan 01 00:00:00 EST 2001}
}
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Works referenced in this record:
Microfabrication of cantilever styli for the atomic force microscope
journal, July 1990
- Albrecht, T. R.; Akamine, S.; Carver, T. E.
- Journal of Vacuum Science & Technology A: Vacuum, Surfaces, and Films, Vol. 8, Issue 4
Detection of mercury vapor using resonating microcantilevers
journal, March 1995
- Thundat, T.; Wachter, E. A.; Sharp, S. L.
- Applied Physics Letters, Vol. 66, Issue 13
Photothermal spectroscopy with femtojoule sensitivity using a micromechanical device
journal, November 1994
- Barnes, J. R.; Stephenson, R. J.; Welland, M. E.
- Nature, Vol. 372, Issue 6501
Sensing Discrete Streptavidin-Biotin Interactions with Atomic Force Microscopy
journal, February 1994
- Lee, Gil U.; Kidwell, David A.; Colton, Richard J.
- Langmuir, Vol. 10, Issue 2
A nondestructive method for determining the spring constant of cantilevers for scanning force microscopy
journal, February 1993
- Cleveland, J. P.; Manne, S.; Bocek, D.
- Review of Scientific Instruments, Vol. 64, Issue 2
A mechanical nanosensor in the gigahertz range: where mechanics meets electronics
journal, January 1994
- Binh, Vu Thien; Garcia, N.; Levanuyk, A. L.
- Surface Science, Vol. 301, Issue 1-3
Observation of a chemical reaction using a micromechanical sensor
journal, January 1994
- Gimzewski, J. K.; Gerber, Ch.; Meyer, E.
- Chemical Physics Letters, Vol. 217, Issue 5-6, p. 589-594
Viscous drag measurements utilizing microfabricated cantilevers
journal, June 1996
- Oden, P. I.; Chen, G. Y.; Steele, R. A.
- Applied Physics Letters, Vol. 68, Issue 26
Uncooled thermal imaging using a piezoresistive microcantilever
journal, November 1996
- Oden, P. I.; Datskos, P. G.; Thundat, T.
- Applied Physics Letters, Vol. 69, Issue 21