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Title: Micromechanical antibody sensor

Abstract

A sensor apparatus is provided using a microcantilevered spring element having a coating of a detector molecule such as an antibody or antigen. A sample containing a target molecule or substrate is provided to the coating. The spring element bends in response to the stress induced by the binding which occurs between the detector and target molecules. Deflections of the cantilever are detected by a variety of detection techniques. The microcantilever may be approximately 1 to 200 .mu.m long, approximately 1 to 50 .mu.m wide, and approximately 0.3 to 3.0 .mu.m thick. A sensitivity for detection of deflections is in the range of 0.01 nanometers.

Inventors:
 [1];  [2];  [1];  [2];  [1]
  1. Knoxville, TN
  2. Oak Ridge, TN
Issue Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
OSTI Identifier:
873996
Patent Number(s):
6289717
Assignee:
U. T. Battelle, LLC (Oak Ridge, TN)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
AC05-96OR22464
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
micromechanical; antibody; sensor; apparatus; provided; microcantilevered; spring; element; coating; detector; molecule; antigen; sample; containing; target; substrate; bends; response; stress; induced; binding; occurs; molecules; deflections; cantilever; detected; variety; detection; techniques; microcantilever; approximately; 200; 50; wide; thick; sensitivity; range; 01; nanometers; detection techniques; spring element; sample containing; sensor apparatus; stress induced; microcantilevered spring; target molecule; /73/422/436/

Citation Formats

Thundat, Thomas G, Jacobson, K Bruce, Doktycz, Mitchel J, Kennel, Stephen J, and Warmack, Robert J. Micromechanical antibody sensor. United States: N. p., 2001. Web.
Thundat, Thomas G, Jacobson, K Bruce, Doktycz, Mitchel J, Kennel, Stephen J, & Warmack, Robert J. Micromechanical antibody sensor. United States.
Thundat, Thomas G, Jacobson, K Bruce, Doktycz, Mitchel J, Kennel, Stephen J, and Warmack, Robert J. Mon . "Micromechanical antibody sensor". United States. https://www.osti.gov/servlets/purl/873996.
@article{osti_873996,
title = {Micromechanical antibody sensor},
author = {Thundat, Thomas G and Jacobson, K Bruce and Doktycz, Mitchel J and Kennel, Stephen J and Warmack, Robert J},
abstractNote = {A sensor apparatus is provided using a microcantilevered spring element having a coating of a detector molecule such as an antibody or antigen. A sample containing a target molecule or substrate is provided to the coating. The spring element bends in response to the stress induced by the binding which occurs between the detector and target molecules. Deflections of the cantilever are detected by a variety of detection techniques. The microcantilever may be approximately 1 to 200 .mu.m long, approximately 1 to 50 .mu.m wide, and approximately 0.3 to 3.0 .mu.m thick. A sensitivity for detection of deflections is in the range of 0.01 nanometers.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2001},
month = {1}
}

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Works referenced in this record:

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