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Title: Nucleic acid arrays and methods of synthesis

Abstract

The present invention generally relates to high density nucleic acid arrays and methods of synthesizing nucleic acid sequences on a solid surface. Specifically, the present invention contemplates the use of stabilized nucleic acid primer sequences immobilized on solid surfaces, and circular nucleic acid sequence templates combined with the use of isothermal rolling circle amplification to thereby increase nucleic acid sequence concentrations in a sample or on an array of nucleic acid sequences.

Inventors:
 [1];  [2];  [3];  [4];  [5]
  1. Allston, MA
  2. Needham, MA
  3. Syracuse, NY
  4. Seattle, WA
  5. Del Mar, CA
Issue Date:
Research Org.:
Boston University
OSTI Identifier:
873967
Patent Number(s):
6284497
Assignee:
Trustees of Boston University (Boston, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
DOE Contract Number:  
FG02-93ER61609
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
nucleic; acid; arrays; methods; synthesis; relates; density; synthesizing; sequences; solid; surface; specifically; contemplates; stabilized; primer; immobilized; surfaces; circular; sequence; templates; combined; isothermal; rolling; circle; amplification; increase; concentrations; sample; array; solid surface; acid sequence; nucleic acid; acid sequences; solid surfaces; acid arrays; /435/436/999/

Citation Formats

Sabanayagam, Chandran R, Sano, Takeshi, Misasi, John, Hatch, Anson, and Cantor, Charles. Nucleic acid arrays and methods of synthesis. United States: N. p., 2001. Web.
Sabanayagam, Chandran R, Sano, Takeshi, Misasi, John, Hatch, Anson, & Cantor, Charles. Nucleic acid arrays and methods of synthesis. United States.
Sabanayagam, Chandran R, Sano, Takeshi, Misasi, John, Hatch, Anson, and Cantor, Charles. Mon . "Nucleic acid arrays and methods of synthesis". United States. https://www.osti.gov/servlets/purl/873967.
@article{osti_873967,
title = {Nucleic acid arrays and methods of synthesis},
author = {Sabanayagam, Chandran R and Sano, Takeshi and Misasi, John and Hatch, Anson and Cantor, Charles},
abstractNote = {The present invention generally relates to high density nucleic acid arrays and methods of synthesizing nucleic acid sequences on a solid surface. Specifically, the present invention contemplates the use of stabilized nucleic acid primer sequences immobilized on solid surfaces, and circular nucleic acid sequence templates combined with the use of isothermal rolling circle amplification to thereby increase nucleic acid sequence concentrations in a sample or on an array of nucleic acid sequences.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2001},
month = {1}
}

Works referenced in this record:

Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction
journal, January 1986


Signal amplification of padlock probes by rolling circle replication
journal, November 1998


Rolling replication of short DNA circles.
journal, May 1995


Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format.
journal, February 1989