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Title: Nucleic acid arrays and methods of synthesis

Abstract

The present invention generally relates to high density nucleic acid arrays and methods of synthesizing nucleic acid sequences on a solid surface. Specifically, the present invention contemplates the use of stabilized nucleic acid primer sequences immobilized on solid surfaces, and circular nucleic acid sequence templates combined with the use of isothermal rolling circle amplification to thereby increase nucleic acid sequence concentrations in a sample or on an array of nucleic acid sequences.

Inventors:
 [1];  [2];  [3];  [4];  [5]
  1. (Allston, MA)
  2. (Needham, MA)
  3. (Syracuse, NY)
  4. (Seattle, WA)
  5. (Del Mar, CA)
Issue Date:
Research Org.:
Boston University
OSTI Identifier:
873967
Patent Number(s):
6284497
Assignee:
Trustees of Boston University (Boston, MA) CHO
DOE Contract Number:  
FG02-93ER61609
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
nucleic; acid; arrays; methods; synthesis; relates; density; synthesizing; sequences; solid; surface; specifically; contemplates; stabilized; primer; immobilized; surfaces; circular; sequence; templates; combined; isothermal; rolling; circle; amplification; increase; concentrations; sample; array; solid surface; acid sequence; nucleic acid; acid sequences; solid surfaces; acid arrays; /435/436/999/

Citation Formats

Sabanayagam, Chandran R., Sano, Takeshi, Misasi, John, Hatch, Anson, and Cantor, Charles. Nucleic acid arrays and methods of synthesis. United States: N. p., 2001. Web.
Sabanayagam, Chandran R., Sano, Takeshi, Misasi, John, Hatch, Anson, & Cantor, Charles. Nucleic acid arrays and methods of synthesis. United States.
Sabanayagam, Chandran R., Sano, Takeshi, Misasi, John, Hatch, Anson, and Cantor, Charles. Mon . "Nucleic acid arrays and methods of synthesis". United States. https://www.osti.gov/servlets/purl/873967.
@article{osti_873967,
title = {Nucleic acid arrays and methods of synthesis},
author = {Sabanayagam, Chandran R. and Sano, Takeshi and Misasi, John and Hatch, Anson and Cantor, Charles},
abstractNote = {The present invention generally relates to high density nucleic acid arrays and methods of synthesizing nucleic acid sequences on a solid surface. Specifically, the present invention contemplates the use of stabilized nucleic acid primer sequences immobilized on solid surfaces, and circular nucleic acid sequence templates combined with the use of isothermal rolling circle amplification to thereby increase nucleic acid sequence concentrations in a sample or on an array of nucleic acid sequences.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2001},
month = {1}
}

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