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Title: Hybridization and sequencing of nucleic acids using base pair mismatches

Abstract

Devices and techniques for hybridization of nucleic acids and for determining the sequence of nucleic acids. Arrays of nucleic acids are formed by techniques, preferably high resolution, light-directed techniques. Positions of hybridization of a target nucleic acid are determined by, e.g., epifluorescence microscopy. Devices and techniques are proposed to determine the sequence of a target nucleic acid more efficiently and more quickly through such synthesis and detection techniques.

Inventors:
 [1];  [1];  [2]
  1. Palo Alto, CA
  2. Mt. View, CA
Issue Date:
Research Org.:
Affymax Research Institute
OSTI Identifier:
873966
Patent Number(s):
6284460
Assignee:
Affymetrix Inc. (Santa Clara, CA)
Patent Classifications (CPCs):
G - PHYSICS G16 - INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS G16B - BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
DOE Contract Number:  
FG03-92ER81275
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
hybridization; sequencing; nucleic; acids; base; pair; mismatches; devices; techniques; determining; sequence; arrays; formed; preferably; resolution; light-directed; positions; target; acid; determined; epifluorescence; microscopy; proposed; determine; efficiently; quickly; synthesis; detection; detection techniques; nucleic acid; nucleic acids; target nucleic; base pair; epifluorescence microscopy; light-directed techniques; target nuclei; /435/999/

Citation Formats

Fodor, Stephen P. A., Lipshutz, Robert J, and Huang, Xiaohua. Hybridization and sequencing of nucleic acids using base pair mismatches. United States: N. p., 2001. Web.
Fodor, Stephen P. A., Lipshutz, Robert J, & Huang, Xiaohua. Hybridization and sequencing of nucleic acids using base pair mismatches. United States.
Fodor, Stephen P. A., Lipshutz, Robert J, and Huang, Xiaohua. Mon . "Hybridization and sequencing of nucleic acids using base pair mismatches". United States. https://www.osti.gov/servlets/purl/873966.
@article{osti_873966,
title = {Hybridization and sequencing of nucleic acids using base pair mismatches},
author = {Fodor, Stephen P. A. and Lipshutz, Robert J and Huang, Xiaohua},
abstractNote = {Devices and techniques for hybridization of nucleic acids and for determining the sequence of nucleic acids. Arrays of nucleic acids are formed by techniques, preferably high resolution, light-directed techniques. Positions of hybridization of a target nucleic acid are determined by, e.g., epifluorescence microscopy. Devices and techniques are proposed to determine the sequence of a target nucleic acid more efficiently and more quickly through such synthesis and detection techniques.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2001},
month = {1}
}

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Works referenced in this record:

Sequencing of megabase plus DNA by hybridization: Theory of the method
journal, February 1989


Sequencing of pools of nucleic acids on oligonucleotide arrays
journal, January 1993


Likelihood DNA Sequencing By Hybridization
journal, December 1993


An oligonucleotide hybridization approach to DNA sequencing
journal, October 1989


DNA sequencing with chain-terminating inhibitors
journal, December 1977


A new method for sequencing DNA.
journal, February 1977