Method and apparatus for rapid biohydrogen phenotypic screening of microorganisms using a chemochromic sensor
Abstract
The invention provides an assay system for identifying a hydrogen-gas-producing organism, including a sensor film having a first layer comprising a transition metal oxide or oxysalt and a second layer comprising hydrogen-dissociative catalyst metal, the first and second layers having an inner and an outer surface wherein the inner surface of the second layer is deposited on the outer surface of the first layer, and a substrate disposed proximally to the outer surface of the second layer, the organism being isolated on the substrate.
- Inventors:
-
- Lakewood, CO
- Golden, CO
- Issue Date:
- Research Org.:
- Midwest Research Institute, Kansas City, MO (United States)
- OSTI Identifier:
- 873944
- Patent Number(s):
- 6277589
- Assignee:
- Midwest Research Institute (Kansas City, MO)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- AC36-98GO10337
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- method; apparatus; rapid; biohydrogen; phenotypic; screening; microorganisms; chemochromic; sensor; provides; assay; identifying; hydrogen-gas-producing; organism; including; film; layer; comprising; transition; metal; oxide; oxysalt; hydrogen-dissociative; catalyst; layers; inner; outer; surface; deposited; substrate; disposed; proximally; isolated; catalyst metal; substrate disposed; layer comprising; metal oxide; inner surface; transition metal; outer surface; comprising hydrogen; hydrogen ph; /435/204/205/
Citation Formats
Seibert, Michael, Benson, David K, and Flynn, Timothy Michael. Method and apparatus for rapid biohydrogen phenotypic screening of microorganisms using a chemochromic sensor. United States: N. p., 2001.
Web.
Seibert, Michael, Benson, David K, & Flynn, Timothy Michael. Method and apparatus for rapid biohydrogen phenotypic screening of microorganisms using a chemochromic sensor. United States.
Seibert, Michael, Benson, David K, and Flynn, Timothy Michael. Mon .
"Method and apparatus for rapid biohydrogen phenotypic screening of microorganisms using a chemochromic sensor". United States. https://www.osti.gov/servlets/purl/873944.
@article{osti_873944,
title = {Method and apparatus for rapid biohydrogen phenotypic screening of microorganisms using a chemochromic sensor},
author = {Seibert, Michael and Benson, David K and Flynn, Timothy Michael},
abstractNote = {The invention provides an assay system for identifying a hydrogen-gas-producing organism, including a sensor film having a first layer comprising a transition metal oxide or oxysalt and a second layer comprising hydrogen-dissociative catalyst metal, the first and second layers having an inner and an outer surface wherein the inner surface of the second layer is deposited on the outer surface of the first layer, and a substrate disposed proximally to the outer surface of the second layer, the organism being isolated on the substrate.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2001},
month = {1}
}
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Works referenced in this record:
Generation of C. reinhardtii Mutants that Photoproduce H2 from H2O in the Presence of O2
book, January 1998
- Ghirardi, M. L.; Flynn, T.; Forestier, M.
- Photosynthesis: Mechanisms and Effects, p. 1959-1962