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Title: Method for introducing unidirectional nested deletions

Abstract

Disclosed is a method for the introduction of unidirectional deletions in a cloned DNA segment in the context of a cloning vector which contains an f1 endonuclease recognition sequence adjacent to the insertion site of the DNA segment. Also disclosed is a method for producing single-stranded DNA probes utilizing the same cloning vector. An optimal vector, PZIP is described. Methods for introducing unidirectional deletions into a terminal location of a cloned DNA sequence which is inserted into the vector of the present invention are also disclosed. These methods are useful for introducing deletions into either or both ends of a cloned DNA insert, for high throughput sequencing of any DNA of interest.

Inventors:
 [1];  [2];  [3]
  1. (Bellport, NY)
  2. (Horseheads, NY)
  3. (New York, NY)
Issue Date:
Research Org.:
Brookhaven National Laboratory (BNL), Upton, NY
OSTI Identifier:
873796
Patent Number(s):
6248569
Assignee:
Brookhaven Science Associates (Upton, NY) BNL
DOE Contract Number:  
AC02-98CH10886
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
method; introducing; unidirectional; nested; deletions; disclosed; introduction; cloned; dna; segment; context; cloning; vector; contains; f1; endonuclease; recognition; sequence; adjacent; insertion; site; producing; single-stranded; probes; utilizing; optimal; pzip; described; methods; terminal; location; inserted; useful; insert; throughput; sequencing; stranded dna; dna probes; dna sequence; dna segment; cloning vector; cloned dna; single-stranded dna; recognition sequence; sequence adjacent; producing single-stranded; nested deletions; unidirectional deletions; f1 endonuclease; dna probe; endonuclease recognition; introducing unidirectional; unidirectional nested; insertion site; nuclease recognition; producing single; dna insert; /435/

Citation Formats

Dunn, John J., Quesada, Mark A., and Randesi, Matthew. Method for introducing unidirectional nested deletions. United States: N. p., 2001. Web.
Dunn, John J., Quesada, Mark A., & Randesi, Matthew. Method for introducing unidirectional nested deletions. United States.
Dunn, John J., Quesada, Mark A., and Randesi, Matthew. Mon . "Method for introducing unidirectional nested deletions". United States. https://www.osti.gov/servlets/purl/873796.
@article{osti_873796,
title = {Method for introducing unidirectional nested deletions},
author = {Dunn, John J. and Quesada, Mark A. and Randesi, Matthew},
abstractNote = {Disclosed is a method for the introduction of unidirectional deletions in a cloned DNA segment in the context of a cloning vector which contains an f1 endonuclease recognition sequence adjacent to the insertion site of the DNA segment. Also disclosed is a method for producing single-stranded DNA probes utilizing the same cloning vector. An optimal vector, PZIP is described. Methods for introducing unidirectional deletions into a terminal location of a cloned DNA sequence which is inserted into the vector of the present invention are also disclosed. These methods are useful for introducing deletions into either or both ends of a cloned DNA insert, for high throughput sequencing of any DNA of interest.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2001},
month = {1}
}

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