Method for introducing unidirectional nested deletions
Abstract
Disclosed is a method for the introduction of unidirectional deletions in a cloned DNA segment in the context of a cloning vector which contains an f1 endonuclease recognition sequence adjacent to the insertion site of the DNA segment. Also disclosed is a method for producing single-stranded DNA probes utilizing the same cloning vector. An optimal vector, PZIP is described. Methods for introducing unidirectional deletions into a terminal location of a cloned DNA sequence which is inserted into the vector of the present invention are also disclosed. These methods are useful for introducing deletions into either or both ends of a cloned DNA insert, for high throughput sequencing of any DNA of interest.
- Inventors:
-
- Bellport, NY
- Horseheads, NY
- New York, NY
- Issue Date:
- Research Org.:
- Brookhaven National Laboratory (BNL), Upton, NY (United States)
- OSTI Identifier:
- 873796
- Patent Number(s):
- 6248569
- Assignee:
- Brookhaven Science Associates (Upton, NY)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC02-98CH10886
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- method; introducing; unidirectional; nested; deletions; disclosed; introduction; cloned; dna; segment; context; cloning; vector; contains; f1; endonuclease; recognition; sequence; adjacent; insertion; site; producing; single-stranded; probes; utilizing; optimal; pzip; described; methods; terminal; location; inserted; useful; insert; throughput; sequencing; stranded dna; dna probes; dna sequence; dna segment; cloning vector; cloned dna; single-stranded dna; recognition sequence; sequence adjacent; producing single-stranded; nested deletions; unidirectional deletions; f1 endonuclease; dna probe; endonuclease recognition; introducing unidirectional; unidirectional nested; insertion site; nuclease recognition; producing single; dna insert; /435/
Citation Formats
Dunn, John J, Quesada, Mark A, and Randesi, Matthew. Method for introducing unidirectional nested deletions. United States: N. p., 2001.
Web.
Dunn, John J, Quesada, Mark A, & Randesi, Matthew. Method for introducing unidirectional nested deletions. United States.
Dunn, John J, Quesada, Mark A, and Randesi, Matthew. Mon .
"Method for introducing unidirectional nested deletions". United States. https://www.osti.gov/servlets/purl/873796.
@article{osti_873796,
title = {Method for introducing unidirectional nested deletions},
author = {Dunn, John J and Quesada, Mark A and Randesi, Matthew},
abstractNote = {Disclosed is a method for the introduction of unidirectional deletions in a cloned DNA segment in the context of a cloning vector which contains an f1 endonuclease recognition sequence adjacent to the insertion site of the DNA segment. Also disclosed is a method for producing single-stranded DNA probes utilizing the same cloning vector. An optimal vector, PZIP is described. Methods for introducing unidirectional deletions into a terminal location of a cloned DNA sequence which is inserted into the vector of the present invention are also disclosed. These methods are useful for introducing deletions into either or both ends of a cloned DNA insert, for high throughput sequencing of any DNA of interest.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2001},
month = {Mon Jan 01 00:00:00 EST 2001}
}
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