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Title: Isolated spinach ribulose-1,5-bisphosphate carboxylase/oxgenase large subunit .epsilon. n-methyltransferase and method of inactivating ribulose-1,5-bishosphatase .epsilon. n-methyltransferase activity

Abstract

The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) .sup..epsilon. N-methyltansferase (protein methylase III or Rubisco LSMT) from a plant which has a des(methyl) lysyl residue in the LS is disclosed. In addition, the full-length cDNA clones for Rubisco LSMT are disclosed. Transgenic plants and methods of producing same which have the Rubisco LSMT gene inserted into the DNA are also provided. Further, methods of inactivating the enzymatic activity of Rubisco LSMT are also disclosed.

Inventors:
 [1]
  1. Lexington, KY
Issue Date:
Research Org.:
University of Kentucky Research Foundation (Lexington, KY)
OSTI Identifier:
873779
Patent Number(s):
6245541
Assignee:
University of Kentucky Research Foundation (Lexington, KY)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02A - TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
DOE Contract Number:  
FG05-92ER26075
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
isolated; spinach; ribulose-1; 5-bisphosphate; carboxylase; oxgenase; subunit; epsilon; n-methyltransferase; method; inactivating; 5-bishosphatase; activity; sequence; oxygenase; rubisco; n-methyltansferase; protein; methylase; iii; lsmt; plant; methyl; lysyl; residue; disclosed; addition; full-length; cdna; clones; transgenic; plants; methods; producing; inserted; dna; provided; enzymatic; cdna clones; cdna clone; rubisco lsmt; transgenic plants; protein methylase; methylase iii; full-length cdna; n-methyltransferase activity; enzymatic activity; transgenic plant; spinach ribulose-1; inactivating ribulose-1; isolated spinach; /435/530/999/800/

Citation Formats

Houtz, Robert L. Isolated spinach ribulose-1,5-bisphosphate carboxylase/oxgenase large subunit .epsilon. n-methyltransferase and method of inactivating ribulose-1,5-bishosphatase .epsilon. n-methyltransferase activity. United States: N. p., 2001. Web.
Houtz, Robert L. Isolated spinach ribulose-1,5-bisphosphate carboxylase/oxgenase large subunit .epsilon. n-methyltransferase and method of inactivating ribulose-1,5-bishosphatase .epsilon. n-methyltransferase activity. United States.
Houtz, Robert L. Mon . "Isolated spinach ribulose-1,5-bisphosphate carboxylase/oxgenase large subunit .epsilon. n-methyltransferase and method of inactivating ribulose-1,5-bishosphatase .epsilon. n-methyltransferase activity". United States. https://www.osti.gov/servlets/purl/873779.
@article{osti_873779,
title = {Isolated spinach ribulose-1,5-bisphosphate carboxylase/oxgenase large subunit .epsilon. n-methyltransferase and method of inactivating ribulose-1,5-bishosphatase .epsilon. n-methyltransferase activity},
author = {Houtz, Robert L},
abstractNote = {The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) .sup..epsilon. N-methyltansferase (protein methylase III or Rubisco LSMT) from a plant which has a des(methyl) lysyl residue in the LS is disclosed. In addition, the full-length cDNA clones for Rubisco LSMT are disclosed. Transgenic plants and methods of producing same which have the Rubisco LSMT gene inserted into the DNA are also provided. Further, methods of inactivating the enzymatic activity of Rubisco LSMT are also disclosed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2001},
month = {Mon Jan 01 00:00:00 EST 2001}
}

Works referenced in this record:

Light-regulated methylation of chloroplast proteins.
journal, July 1987


Partial Purification and Characterization of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Large Subunit ε N -Methyltransferase
journal, November 1991


Affinity Purification of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Large Subunit ϵN-Methyltransferase
journal, August 1995


Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes
journal, August 1988


Post-translational modifications in the large subunit of ribulose bisphosphate carboxylase/oxygenase.
journal, March 1989


Cloning and developmental expression of the sucrose-phosphate-synthase gene from spinach
journal, June 1993


Protein Methylation in Pea Chloroplasts
journal, July 1990