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Title: Nucleic acid amplification using modular branched primers

Abstract

Methods and compositions expand the options for making primers for use in amplifying nucleic acid segments. The invention eliminates the step of custom synthesis of primers for Polymerase Chain Reactions (PCR). Instead of being custom-synthesized, a primer is replaced by a combination of several oligonucleotide modules selected from a pre-synthesized library. A modular combination of just a few oligonucleotides essentially mimics the performance of a conventional, custom-made primer by matching the sequence of the priming site in the template. Each oligonucleotide module has a segment that matches one of the stretches within the priming site.

Inventors:
 [1]
  1. Westmont, IL
Issue Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
OSTI Identifier:
873756
Patent Number(s):
6235889
Assignee:
University of Chicago (Chicago, IL)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
W-31109-ENG-38
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
nucleic; acid; amplification; modular; branched; primers; methods; compositions; expand; options; amplifying; segments; eliminates; step; custom; synthesis; polymerase; chain; reactions; pcr; custom-synthesized; primer; replaced; combination; oligonucleotide; modules; selected; pre-synthesized; library; oligonucleotides; essentially; mimics; performance; conventional; custom-made; matching; sequence; priming; site; template; module; segment; matches; stretches; acid segments; chain reaction; nucleic acid; polymerase chain; acid amplification; compositions expand; modular branched; branched primers; amplifying nucleic; acid segment; custom synthesis; /999/435/

Citation Formats

Ulanovsky, Levy. Nucleic acid amplification using modular branched primers. United States: N. p., 2001. Web.
Ulanovsky, Levy. Nucleic acid amplification using modular branched primers. United States.
Ulanovsky, Levy. Mon . "Nucleic acid amplification using modular branched primers". United States. https://www.osti.gov/servlets/purl/873756.
@article{osti_873756,
title = {Nucleic acid amplification using modular branched primers},
author = {Ulanovsky, Levy},
abstractNote = {Methods and compositions expand the options for making primers for use in amplifying nucleic acid segments. The invention eliminates the step of custom synthesis of primers for Polymerase Chain Reactions (PCR). Instead of being custom-synthesized, a primer is replaced by a combination of several oligonucleotide modules selected from a pre-synthesized library. A modular combination of just a few oligonucleotides essentially mimics the performance of a conventional, custom-made primer by matching the sequence of the priming site in the template. Each oligonucleotide module has a segment that matches one of the stretches within the priming site.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2001},
month = {1}
}

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Works referenced in this record:

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