Nucleic acid indexing
Abstract
A restriction site indexing method for selectively amplifying any fragment generated by a Class II restriction enzyme includes adaptors specific to fragment ends containing adaptor indexing sequences complementary to fragment indexing sequences near the termini of fragments generated by Class II enzyme cleavage. A method for combinatorial indexing facilitates amplification of restriction fragments whose sequence is not known.
- Inventors:
-
- Madison, WI
- Bellevue, WA
- Issue Date:
- Research Org.:
- Univ. of Wisconsin, Madison, WI (United States)
- OSTI Identifier:
- 873713
- Patent Number(s):
- 6228999
- Assignee:
- Wisconsin Alumni Research Foundation (Madison, WI)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG02-91ER61122
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- nucleic; acid; indexing; restriction; site; method; selectively; amplifying; fragment; generated; ii; enzyme; adaptors; specific; containing; adaptor; sequences; complementary; near; termini; fragments; cleavage; combinatorial; facilitates; amplification; sequence; nucleic acid; restriction enzyme; restriction fragments; restriction site; indexing sequences; selectively amplifying; sequences complementary; site indexing; fragments generated; fragment generated; fragment indexing; indexing method; ii restriction; restriction fragment; acid indexing; adaptors specific; adaptor indexing; containing adaptor; /999/435/
Citation Formats
Guilfoyle, Richard A, and Guo, Zhen. Nucleic acid indexing. United States: N. p., 2001.
Web.
Guilfoyle, Richard A, & Guo, Zhen. Nucleic acid indexing. United States.
Guilfoyle, Richard A, and Guo, Zhen. Mon .
"Nucleic acid indexing". United States. https://www.osti.gov/servlets/purl/873713.
@article{osti_873713,
title = {Nucleic acid indexing},
author = {Guilfoyle, Richard A and Guo, Zhen},
abstractNote = {A restriction site indexing method for selectively amplifying any fragment generated by a Class II restriction enzyme includes adaptors specific to fragment ends containing adaptor indexing sequences complementary to fragment indexing sequences near the termini of fragments generated by Class II enzyme cleavage. A method for combinatorial indexing facilitates amplification of restriction fragments whose sequence is not known.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2001},
month = {Mon Jan 01 00:00:00 EST 2001}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Branch capture reactions: effect of recipient structure
journal, January 1990
- Weinstock, Peter H.; Wetmur, James G.
- Nucleic Acids Research, Vol. 18, Issue 14
Covalently linked sequencing primer (splinkers) for sequence analysis of restriction fragments
journal, January 1986
- Kalisch, Bernd W.; Krawetz, Stephen A.; Schoenwaelder, Karl-Heinz
- Gene, Vol. 44, Issue 2-3
"RFLP subtraction": a method for making libraries of polymorphic markers.
journal, June 1994
- Rosenberg, M.; Przybylska, M.; Straus, D.
- Proceedings of the National Academy of Sciences, Vol. 91, Issue 13
[19] Representational difference analysis in detection of genetic lesions in cancer
book, January 1995
- Lisitsyn, Nikolai; Wigler, Michael
- Oncogene techniques
Branch capture reactions: displacers derived from asymmetric PCR
journal, January 1991
- Wong, Daphne M.; Weinstock, Peter H.; Wetmur, James G.
- Nucleic Acids Research, Vol. 19, Issue 9
Non-cloning amplification of specific DNA fragments from whole genomic DNA digests using DNA ‘indexers’
journal, August 1994
- Unrau, Paul; Deugau, Kenneth V.
- Gene, Vol. 145, Issue 2
Ligation-mediated PCR of restriction fragments from large DNA molecules.
journal, August 1992
- Smith, D. R.
- Genome Research, Vol. 2, Issue 1
Cloning the differences between two complex genomes
journal, February 1993
- Lisitsyn, N.; Lisitsyn, N.; Wigler, M.
- Science, Vol. 259, Issue 5097
[18] Fingerprinting of DNA and RNA by arbitrarily primed polymerase chain reaction: Applications in cancer research
book, January 1995
- Perucho, Manuel; Welsh, John; Peinado, Miguel Angel
- Oncogene techniques
AFLP: a new technique for DNA fingerprinting
journal, January 1995
- Vos, Pieter; Hogers, Rene; Bleeker, Marjo
- Nucleic Acids Research, Vol. 23, Issue 21
Branch migration mediated DNA labeling and cloning
journal, October 1989
- Quartin, Robin S.; Plewinska, Magdalena; Wetmur, James G.
- Biochemistry, Vol. 28, Issue 22
Ligation-mediated PCR amplification of specific fragments from a Class-II restriction endonuclease total digest
journal, May 1997
- Guilfoyle, R. A.; Leeck, C. L.; Kroening, K. D.
- Nucleic Acids Research, Vol. 25, Issue 9
[20] Analysis of altered gene expression by differential display
book, January 1995
- Liang, Peng; Bauer, David; Averboukh, Lidia
- Oncogene techniques