Nucleic acid amplification using modular branched primers
Abstract
Methods and compositions expand the options for making primers for use in amplifying nucleic acid segments. The invention eliminates the step of custom synthesis of primers for Polymerase Chain Reactions (PCR). Instead of being custom-synthesized, a primer is replaced by a combination of several oligonucleotide modules selected from a pre-synthesized library. A modular combination of just a few oligonucleotides essentially mimics the performance of a conventional, custom-made primer by matching the sequence of the priming site in the template. Each oligonucleotide module has a segment that matches one of the stretches within the priming site.
- Inventors:
-
- Westmont, IL
- Downers Grove, IL
- Issue Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- OSTI Identifier:
- 873589
- Patent Number(s):
- 6197556
- Assignee:
- University of Chicago (Chicago, IL)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- W-31109-ENG-38
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- nucleic; acid; amplification; modular; branched; primers; methods; compositions; expand; options; amplifying; segments; eliminates; step; custom; synthesis; polymerase; chain; reactions; pcr; custom-synthesized; primer; replaced; combination; oligonucleotide; modules; selected; pre-synthesized; library; oligonucleotides; essentially; mimics; performance; conventional; custom-made; matching; sequence; priming; site; template; module; segment; matches; stretches; acid segments; chain reaction; nucleic acid; polymerase chain; acid amplification; compositions expand; modular branched; branched primers; amplifying nucleic; acid segment; custom synthesis; /435/999/
Citation Formats
Ulanovsky, Levy, and Raja, Mugasimangalam C. Nucleic acid amplification using modular branched primers. United States: N. p., 2001.
Web.
Ulanovsky, Levy, & Raja, Mugasimangalam C. Nucleic acid amplification using modular branched primers. United States.
Ulanovsky, Levy, and Raja, Mugasimangalam C. Mon .
"Nucleic acid amplification using modular branched primers". United States. https://www.osti.gov/servlets/purl/873589.
@article{osti_873589,
title = {Nucleic acid amplification using modular branched primers},
author = {Ulanovsky, Levy and Raja, Mugasimangalam C},
abstractNote = {Methods and compositions expand the options for making primers for use in amplifying nucleic acid segments. The invention eliminates the step of custom synthesis of primers for Polymerase Chain Reactions (PCR). Instead of being custom-synthesized, a primer is replaced by a combination of several oligonucleotide modules selected from a pre-synthesized library. A modular combination of just a few oligonucleotides essentially mimics the performance of a conventional, custom-made primer by matching the sequence of the priming site in the template. Each oligonucleotide module has a segment that matches one of the stretches within the priming site.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2001},
month = {Mon Jan 01 00:00:00 EST 2001}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
DNA sequencing by primer walking with strings of contiguous hexamers
journal, December 1992
- Kieleczawa, J.; Dunn, J.; Studier, F.
- Science, Vol. 258, Issue 5089
Stability and structure of three-way DNA junctions containing unpaired nucleotides
journal, January 1991
- Leontis, Neocles B.; Kwok, Wendy; Newman, Jennifer S.
- Nucleic Acids Research, Vol. 19, Issue 4
Curved DNA: design, synthesis, and circularization.
journal, February 1986
- Ulanovsky, L.; Bodner, M.; Trifonov, E. N.
- Proceedings of the National Academy of Sciences, Vol. 83, Issue 4
PNA hybridizes to complementary oligonucleotides obeying the Watson–Crick hydrogen-bonding rules
journal, October 1993
- Egholm, Michael; Buchardt, Ole; Christensen, Leif
- Nature, Vol. 365, Issue 6446
Enhanced discrimination of single nucleotide polymorphisms by artificial mismatch hybridization
journal, April 1997
- Guo, Zhen; Liu, Qinghua; Smith, Lloyd M.
- Nature Biotechnology, Vol. 15, Issue 4
Proposal for sequencing DNA using ligation of hexamers to generate sequential elongation primers (SPEL-6)
journal, May 1990
- Szybalski, W.
- Gene, Vol. 90, Issue 1
Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide
journal, December 1991
- Nielsen, P.; Egholm, M.; Berg, R.
- Science, Vol. 254, Issue 5037
DNA trapping electrophoresis
journal, January 1990
- Ulanovsky, Levy; Drouin, Guy; Gilbert, Walter
- Nature, Vol. 343, Issue 6254
Strand displacement amplification—an isothermal, in vitro DNA amplification technique
journal, January 1992
- Walker, G. Terrance; Fraiser, Melinda S.; Schram, James L.
- Nucleic Acids Research, Vol. 20, Issue 7, p. 1691-1696
Empirical aspects of strand displacement amplification.
journal, August 1993
- Walker, G. T.
- Genome Research, Vol. 3, Issue 1
On the mechanism of the modular primer effect
journal, January 1995
- Beskin, Alexander D.; Zevin-Sonkin, Dina; Sobolev, Irina A.
- Nucleic Acids Research, Vol. 23, Issue 15
A strategy for high-volume sequencing of cosmid DNAs: random and directed priming with a library of oligonucleotides.
journal, September 1989
- Studier, F. W.
- Proceedings of the National Academy of Sciences, Vol. 86, Issue 18
DNA sequencing: modular primers assembled from a library of hexamers or pentamers.
journal, May 1993
- Kotler, L. E.; Zevin-Sonkin, D.; Sobolev, I. A.
- Proceedings of the National Academy of Sciences, Vol. 90, Issue 9
DNA sequencing using differential extension with nucleotide subsets (DENS)
journal, February 1997
- Raja, M. C.; Zevin-Sonkin, D.; Shwartzburd, J.
- Nucleic Acids Research, Vol. 25, Issue 4
Estimation of wedge components in curved DNA
journal, April 1987
- Ulanovsky, Levy E.; Trifonov, Edward N.
- Nature, Vol. 326, Issue 6114
The ‘shortmer’ approach to nucleic acid sequence analysis I: computer simulation of sequencing projects to find economical primer sets
journal, January 1994
- Blöcker, Helmut; Lincoln, David N.
- Bioinformatics, Vol. 10, Issue 2
An alternative approach to deoxyoligonucleotides as hybridization probes by insertion of deoxyinosine at ambiguous codon positions.
journal, March 1985
- Ohtsuka, E.; Matsuki, S.; Ikehara, M.
- Journal of Biological Chemistry, Vol. 260, Issue 5
Strings of contiguous modified pentanucleotides with increased DNA-binding affinity can be used for DNA sequencing by primer walking.
journal, December 1993
- Azhikina, T.; Veselovskaya, S.; Myasnikov, V.
- Proceedings of the National Academy of Sciences, Vol. 90, Issue 24