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Title: Nucleic acid amplification using modular branched primers

Abstract

Methods and compositions expand the options for making primers for use in amplifying nucleic acid segments. The invention eliminates the step of custom synthesis of primers for Polymerase Chain Reactions (PCR). Instead of being custom-synthesized, a primer is replaced by a combination of several oligonucleotide modules selected from a pre-synthesized library. A modular combination of just a few oligonucleotides essentially mimics the performance of a conventional, custom-made primer by matching the sequence of the priming site in the template. Each oligonucleotide module has a segment that matches one of the stretches within the priming site.

Inventors:
 [1];  [2]
  1. Westmont, IL
  2. Downers Grove, IL
Issue Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL (United States)
OSTI Identifier:
873589
Patent Number(s):
6197556
Assignee:
University of Chicago (Chicago, IL)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
W-31109-ENG-38
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
nucleic; acid; amplification; modular; branched; primers; methods; compositions; expand; options; amplifying; segments; eliminates; step; custom; synthesis; polymerase; chain; reactions; pcr; custom-synthesized; primer; replaced; combination; oligonucleotide; modules; selected; pre-synthesized; library; oligonucleotides; essentially; mimics; performance; conventional; custom-made; matching; sequence; priming; site; template; module; segment; matches; stretches; acid segments; chain reaction; nucleic acid; polymerase chain; acid amplification; compositions expand; modular branched; branched primers; amplifying nucleic; acid segment; custom synthesis; /435/999/

Citation Formats

Ulanovsky, Levy, and Raja, Mugasimangalam C. Nucleic acid amplification using modular branched primers. United States: N. p., 2001. Web.
Ulanovsky, Levy, & Raja, Mugasimangalam C. Nucleic acid amplification using modular branched primers. United States.
Ulanovsky, Levy, and Raja, Mugasimangalam C. Mon . "Nucleic acid amplification using modular branched primers". United States. https://www.osti.gov/servlets/purl/873589.
@article{osti_873589,
title = {Nucleic acid amplification using modular branched primers},
author = {Ulanovsky, Levy and Raja, Mugasimangalam C},
abstractNote = {Methods and compositions expand the options for making primers for use in amplifying nucleic acid segments. The invention eliminates the step of custom synthesis of primers for Polymerase Chain Reactions (PCR). Instead of being custom-synthesized, a primer is replaced by a combination of several oligonucleotide modules selected from a pre-synthesized library. A modular combination of just a few oligonucleotides essentially mimics the performance of a conventional, custom-made primer by matching the sequence of the priming site in the template. Each oligonucleotide module has a segment that matches one of the stretches within the priming site.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2001},
month = {1}
}

Works referenced in this record:

DNA sequencing by primer walking with strings of contiguous hexamers
journal, December 1992


Stability and structure of three-way DNA junctions containing unpaired nucleotides
journal, January 1991


Curved DNA: design, synthesis, and circularization.
journal, February 1986


PNA hybridizes to complementary oligonucleotides obeying the Watson–Crick hydrogen-bonding rules
journal, October 1993


Enhanced discrimination of single nucleotide polymorphisms by artificial mismatch hybridization
journal, April 1997


Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide
journal, December 1991


DNA trapping electrophoresis
journal, January 1990


Strand displacement amplification—an isothermal, in vitro DNA amplification technique
journal, January 1992


Empirical aspects of strand displacement amplification.
journal, August 1993


On the mechanism of the modular primer effect
journal, January 1995


A strategy for high-volume sequencing of cosmid DNAs: random and directed priming with a library of oligonucleotides.
journal, September 1989


DNA sequencing: modular primers assembled from a library of hexamers or pentamers.
journal, May 1993


DNA sequencing using differential extension with nucleotide subsets (DENS)
journal, February 1997


Estimation of wedge components in curved DNA
journal, April 1987


Strings of contiguous modified pentanucleotides with increased DNA-binding affinity can be used for DNA sequencing by primer walking.
journal, December 1993