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Title: Nucleic and amino acid sequences relating to a novel transketolase, and methods for the expression thereof

cDNAs encoding 1-deoxyxylulose-5-phosphate synthase from peppermint (Mentha piperita) have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7) are provided which code for the expression of 1-deoxyxylulose-5-phosphate synthase from plants. In another aspect the present invention provides for isolated, recombinant DXPS proteins, such as the proteins having the sequences set forth in SEQ ID NO:4, SEQ ID NO:6 and SEQ ID NO:8. In other aspects, replicable recombinant cloning vehicles are provided which code for plant 1-deoxyxylulose-5-phosphate synthases, or for a base sequence sufficiently complementary to at least a portion of 1-deoxyxylulose-5-phosphate synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding a plant 1-deoxyxylulose-5-phosphate synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant 1-deoxyxylulose-5-phosphate synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant 1-deoxyxylulose-5-phosphate synthase may be used to obtain expression or enhanced expression of 1-deoxyxylulose-5-phosphate synthase in plants in order to enhance the production ofmore » 1-deoxyxylulose-5-phosphate, or its derivatives such as isopentenyl diphosphate (BP), or may be otherwise employed for the regulation or expression of 1-deoxyxylulose-5-phosphate synthase, or the production of its products.« less
Inventors:
 [1];  [2];  [1];  [1]
  1. (Pullman, WA)
  2. (Colfax, WA)
Issue Date:
OSTI Identifier:
873567
Assignee:
Washington State University Research Foundation (Pullman, WA) OAK
Patent Number(s):
US 6190895
Contract Number:
FG03-96ER20212
Research Org:
Washington State University Research Foundation (Pullman, WA)
Country of Publication:
United States
Language:
English
Subject:
nucleic; amino; acid; sequences; relating; novel; transketolase; methods; expression; cdnas; encoding; 1-deoxyxylulose-5-phosphate; synthase; peppermint; mentha; piperita; isolated; sequenced; corresponding; determined; accordingly; dna; seq; provided; code; plants; aspect; provides; recombinant; dxps; proteins; set; forth; aspects; replicable; cloning; vehicles; plant; synthases; base; sequence; sufficiently; complementary; portion; rna; enable; hybridization; therewith; modified; host; cells; transformed; transfected; infected; injected; vehicle; systems; aforementioned; facilitate; production; isolation; purification; significant; amounts; obtain; enhanced; enhance; derivatives; isopentenyl; diphosphate; bp; otherwise; employed; regulation; products; sequence sufficiently; modified host; cloning vehicles; recombinant expression; replicable recombinant; obtain expression; otherwise employed; enable hybridization; hybridization therewith; synthase dna; recombinant cloning; dna sequences; dna sequence; isolated dna; significant amounts; acid sequence; amino acid; acid sequences; host cells; sequence encoding; base sequence; cloning vehicle; host cell; sufficiently complementary; set forth; cdnas encoding; significant amount; mentha piperita; corresponding amino; enhanced expression; aforementioned recombinant; /435/999/