Advanced surface-enhanced Raman gene probe systems and methods thereof
Abstract
The subject invention is a series of methods and systems for using the Surface-Enhanced Raman (SER)-labeled Gene Probe for hybridization, detection and identification of SER-labeled hybridized target oligonucleotide material comprising the steps of immobilizing SER-labeled hybridized target oligonucleotide material on a support means, wherein the SER-labeled hybridized target oligonucleotide material comprise a SER label attached either to a target oligonucleotide of unknown sequence or to a gene probe of known sequence complementary to the target oligonucleotide sequence, the SER label is unique for the target oligonucleotide strands of a particular sequence wherein the SER-labeled oligonucleotide is hybridized to its complementary oligonucleotide strand, then the support means having the SER-labeled hybridized target oligonucleotide material adsorbed thereon is SERS activated with a SERS activating means, then the support means is analyzed.
- Inventors:
-
- Knoxville, TN
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- OSTI Identifier:
- 873496
- Patent Number(s):
- 6174677
- Assignee:
- UT-Battelle, LLC (Oak Ridge, TN)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- AC05-84OR21400
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- advanced; surface-enhanced; raman; probe; systems; methods; subject; series; ser; -labeled; hybridization; detection; identification; ser-labeled; hybridized; target; oligonucleotide; material; comprising; steps; immobilizing; support; means; comprise; label; attached; sequence; complementary; unique; strands; particular; strand; adsorbed; thereon; sers; activated; activating; analyzed; oligonucleotide strand; hybridized target; surface-enhanced raman; nucleotide sequence; material comprising; support means; enhanced raman; oligonucleotide strands; adsorbed thereon; nucleotide strand; nucleotide strands; /435/356/
Citation Formats
Vo-Dinh, Tuan. Advanced surface-enhanced Raman gene probe systems and methods thereof. United States: N. p., 2001.
Web.
Vo-Dinh, Tuan. Advanced surface-enhanced Raman gene probe systems and methods thereof. United States.
Vo-Dinh, Tuan. Mon .
"Advanced surface-enhanced Raman gene probe systems and methods thereof". United States. https://www.osti.gov/servlets/purl/873496.
@article{osti_873496,
title = {Advanced surface-enhanced Raman gene probe systems and methods thereof},
author = {Vo-Dinh, Tuan},
abstractNote = {The subject invention is a series of methods and systems for using the Surface-Enhanced Raman (SER)-labeled Gene Probe for hybridization, detection and identification of SER-labeled hybridized target oligonucleotide material comprising the steps of immobilizing SER-labeled hybridized target oligonucleotide material on a support means, wherein the SER-labeled hybridized target oligonucleotide material comprise a SER label attached either to a target oligonucleotide of unknown sequence or to a gene probe of known sequence complementary to the target oligonucleotide sequence, the SER label is unique for the target oligonucleotide strands of a particular sequence wherein the SER-labeled oligonucleotide is hybridized to its complementary oligonucleotide strand, then the support means having the SER-labeled hybridized target oligonucleotide material adsorbed thereon is SERS activated with a SERS activating means, then the support means is analyzed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2001},
month = {Mon Jan 01 00:00:00 EST 2001}
}
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Surface-enhanced Raman spectroscopy using metallic nanostructures1The submitted manuscript has been authored by a contractor of the U.S Government under contract No. DE-AC05-96OR22464. Accordingly, the U.S. Government retains a nonexclusive, royalty-free license to publish or reproduce the published form of this contribution, or allow others to do so, for U.S. Government purposes.1
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