Method for construction of bacterial strains with increased succinic acid production
Abstract
A fermentation process for producing succinic acid is provided comprising selecting a bacterial strain that does not produce succinic acid in high yield, disrupting the normal regulation of sugar metabolism of said bacterial strain, and combining the mutant bacterial strain and selected sugar in anaerobic conditions to facilitate production of succinic acid. Also provided is a method for changing low yield succinic acid producing bacteria to high yield succinic acid producing bacteria comprising selecting a bacterial strain having a phosphotransferase system and altering the phosphotransferase system so as to allow the bacterial strain to simultaneously metabolize different sugars.
- Inventors:
-
- Warrenville, IL
- Plainfield, IL
- Park Ridge, IL
- Issue Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- OSTI Identifier:
- 873440
- Patent Number(s):
- 6159738
- Assignee:
- University of Chicago (Chicago, IL)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- W-31109-ENG-38
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- method; construction; bacterial; strains; increased; succinic; acid; production; fermentation; process; producing; provided; comprising; selecting; strain; produce; yield; disrupting; normal; regulation; sugar; metabolism; combining; mutant; selected; anaerobic; conditions; facilitate; changing; bacteria; phosphotransferase; altering; allow; simultaneously; metabolize; sugars; acid production; bacterial strain; fermentation process; succinic acid; provided comprising; anaerobic conditions; comprising selecting; acid producing; producing bacteria; increased succinic; bacterial strains; aerobic conditions; /435/999/
Citation Formats
Donnelly, Mark I, Sanville-Millard, Cynthia, and Chatterjee, Ranjini. Method for construction of bacterial strains with increased succinic acid production. United States: N. p., 2000.
Web.
Donnelly, Mark I, Sanville-Millard, Cynthia, & Chatterjee, Ranjini. Method for construction of bacterial strains with increased succinic acid production. United States.
Donnelly, Mark I, Sanville-Millard, Cynthia, and Chatterjee, Ranjini. Sat .
"Method for construction of bacterial strains with increased succinic acid production". United States. https://www.osti.gov/servlets/purl/873440.
@article{osti_873440,
title = {Method for construction of bacterial strains with increased succinic acid production},
author = {Donnelly, Mark I and Sanville-Millard, Cynthia and Chatterjee, Ranjini},
abstractNote = {A fermentation process for producing succinic acid is provided comprising selecting a bacterial strain that does not produce succinic acid in high yield, disrupting the normal regulation of sugar metabolism of said bacterial strain, and combining the mutant bacterial strain and selected sugar in anaerobic conditions to facilitate production of succinic acid. Also provided is a method for changing low yield succinic acid producing bacteria to high yield succinic acid producing bacteria comprising selecting a bacterial strain having a phosphotransferase system and altering the phosphotransferase system so as to allow the bacterial strain to simultaneously metabolize different sugars.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2000},
month = {Sat Jan 01 00:00:00 EST 2000}
}
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Works referenced in this record:
Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria.
journal, January 1993
- Postma, P. W.; Lengeler, J. W.; Jacobson, G. R.
- Microbiological Reviews, Vol. 57, Issue 3