Waveguide detection of right-angle-scattered light in flow cytometry

Title: Waveguide detection of right-angle-scattered light in flow cytometry

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Abstract

A transparent flow cell is used as an index-guided optical waveguide. A detector for the flow cell but not the liquid stream detects the Right-Angle-Scattered (RAS) Light exiting from one end of the flow cell. The detector(s) could view the trapped RAS light from the flow cell either directly or through intermediate optical light guides. If the light exits one end of the flow cell, then the other end of the flow cell can be given a high-reflectivity coating to approximately double the amount of light collected. This system is more robust in its alignment than the traditional flow cytometry systems which use imaging optics, such as microscope objectives.

Inventors:

Mariella, Jr., Raymond P. ^[1]

(Danville, CA)

Issue Date:: 2000-01-01

Research Org.:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

OSTI Identifier:: 873418

Patent Number(s):: 6154276

Assignee:: Regents of University of California (Oakland, CA)

Patent Classifications (CPCs):: G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

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G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N15/1436 - {the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell}

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DOE Contract Number:: W-7405-ENG-48

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: waveguide; detection; right-angle-scattered; light; flow; cytometry; transparent; cell; index-guided; optical; detector; liquid; stream; detects; ras; exiting; view; trapped; directly; intermediate; guides; exits; high-reflectivity; coating; approximately; double; amount; collected; robust; alignment; traditional; systems; imaging; optics; microscope; objectives; imaging optics; liquid stream; scattered light; flow cell; light guide; optical waveguide; flow cytometry; optical light; optical wave; microscope objective; light guides; right-angle-scattered light; light collected; /356/

Citation Formats


                    Mariella, Jr., Raymond P.. Waveguide detection of right-angle-scattered light in flow cytometry.  United States: N. p., 2000. 
        Web.

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                    Mariella, Jr., Raymond P.. Waveguide detection of right-angle-scattered light in flow cytometry.  United States.

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                    Mariella, Jr., Raymond P.. Sat .  
        "Waveguide detection of right-angle-scattered light in flow cytometry".  United States.  https://www.osti.gov/servlets/purl/873418.

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@article{osti_873418,

  title        = {Waveguide detection of right-angle-scattered light in flow cytometry},

  author       = {Mariella, Jr., Raymond P.},

  abstractNote = {A transparent flow cell is used as an index-guided optical waveguide. A detector for the flow cell but not the liquid stream detects the Right-Angle-Scattered (RAS) Light exiting from one end of the flow cell. The detector(s) could view the trapped RAS light from the flow cell either directly or through intermediate optical light guides. If the light exits one end of the flow cell, then the other end of the flow cell can be given a high-reflectivity coating to approximately double the amount of light collected. This system is more robust in its alignment than the traditional flow cytometry systems which use imaging optics, such as microscope objectives.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2000},

  month        = {1}

}

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