skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Modified pseudomonas oleovorans phaC1 nucleic acids encoding bispecific polyhydroxyalkanoate polymerase

Abstract

A genetically engineered Pseudomonas oleovorans phaC1 polyhydroxyalkanoate (PHA) polymerase having tailored substrate specificity is provided. The modified PHA polymerase is preferably a "bispecific" PHA polymerase capable of copolymerizing a short chain length monomer and a medium chain length monomer is provided. Methods for making the modified PHA polymerase and for making nucleic acids encoding the modified PHA polymerase are also disclosed, as are methods of producing PHA using the modified PHA polymerase. The invention further includes methods to assay for altered substrate specificity.

Inventors:
 [1];  [2];  [3]
  1. (Lake Elmo, MN)
  2. (Plymouth, MN)
  3. (Roseville, MN)
Issue Date:
Research Org.:
The Consortium for Plant Biotechnology Research, Inc., West Lafayette, IN (US)
OSTI Identifier:
873364
Patent Number(s):
6143952
Assignee:
Regents of University of Minnesota (Minneapolis, MN) ORO
DOE Contract Number:  
FC05-92OR22072
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
modified; pseudomonas; oleovorans; phac1; nucleic; acids; encoding; bispecific; polyhydroxyalkanoate; polymerase; genetically; engineered; pha; tailored; substrate; specificity; provided; preferably; capable; copolymerizing; chain; length; monomer; medium; methods; disclosed; producing; assay; altered; pha polymerase; acids encoding; nucleic acid; nucleic acids; chain length; genetically engineered; pseudomonas oleovorans; oleovorans phac1; /800/435/999/

Citation Formats

Srienc, Friedrich, Jackson, John K., and Somers, David A. Modified pseudomonas oleovorans phaC1 nucleic acids encoding bispecific polyhydroxyalkanoate polymerase. United States: N. p., 2000. Web.
Srienc, Friedrich, Jackson, John K., & Somers, David A. Modified pseudomonas oleovorans phaC1 nucleic acids encoding bispecific polyhydroxyalkanoate polymerase. United States.
Srienc, Friedrich, Jackson, John K., and Somers, David A. Sat . "Modified pseudomonas oleovorans phaC1 nucleic acids encoding bispecific polyhydroxyalkanoate polymerase". United States. https://www.osti.gov/servlets/purl/873364.
@article{osti_873364,
title = {Modified pseudomonas oleovorans phaC1 nucleic acids encoding bispecific polyhydroxyalkanoate polymerase},
author = {Srienc, Friedrich and Jackson, John K. and Somers, David A.},
abstractNote = {A genetically engineered Pseudomonas oleovorans phaC1 polyhydroxyalkanoate (PHA) polymerase having tailored substrate specificity is provided. The modified PHA polymerase is preferably a "bispecific" PHA polymerase capable of copolymerizing a short chain length monomer and a medium chain length monomer is provided. Methods for making the modified PHA polymerase and for making nucleic acids encoding the modified PHA polymerase are also disclosed, as are methods of producing PHA using the modified PHA polymerase. The invention further includes methods to assay for altered substrate specificity.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {1}
}

Patent:

Save / Share: