skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: High speed flow cytometer droplet formation system and method

Abstract

A droplet forming flow cytometer system allows high speed processing without the need for high oscillator drive powers through the inclusion of an oscillator or piezoelectric crystal such as within the nozzle volume or otherwise unidirectionally coupled to the sheath fluid. The nozzle container continuously converges so as to amplify unidirectional oscillations which are transmitted as pressure waves through the nozzle volume to the nozzle exit so as to form droplets from the fluid jet. The oscillator is directionally isolated so as to avoid moving the entire nozzle container so as to create only pressure waves within the sheath fluid. A variation in substance concentration is achieved through a movable substance introduction port which is positioned within a convergence zone to vary the relative concentration of substance to sheath fluid while still maintaining optimal laminar flow conditions. This variation may be automatically controlled through a sensor and controller configuration. A replaceable tip design is also provided whereby the ceramic nozzle tip is positioned within an edge insert in the nozzle body so as to smoothly transition from nozzle body to nozzle tip. The nozzle tip is sealed against its outer surface to the nozzle body so it may be removablemore » for cleaning or replacement.« less

Inventors:
 [1]
  1. (Seattle, WA)
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
873315
Patent Number(s):
6133044
Assignee:
University of Washington (Seattle, WA) LLNL
DOE Contract Number:  
FG06-93ER61662
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
speed; flow; cytometer; droplet; formation; method; forming; allows; processing; oscillator; drive; powers; inclusion; piezoelectric; crystal; nozzle; volume; otherwise; unidirectionally; coupled; sheath; fluid; container; continuously; converges; amplify; unidirectional; oscillations; transmitted; pressure; waves; exit; form; droplets; jet; directionally; isolated; avoid; moving; entire; create; variation; substance; concentration; achieved; movable; introduction; positioned; convergence; zone; vary; relative; maintaining; optimal; laminar; conditions; automatically; controlled; sensor; controller; configuration; replaceable; tip; design; provided; whereby; ceramic; edge; insert; smoothly; transition; sealed; outer; surface; removable; cleaning; replacement; fluid jet; droplet formation; nozzle tip; nozzle exit; pressure waves; provided whereby; outer surface; flow cytometer; laminar flow; sheath fluid; piezoelectric crystal; flow conditions; pressure wave; automatically control; relative concentration; nozzle container; smoothly transition; ceramic nozzle; form droplets; speed flow; /436/209/324/356/422/

Citation Formats

Van den Engh, Ger. High speed flow cytometer droplet formation system and method. United States: N. p., 2000. Web.
Van den Engh, Ger. High speed flow cytometer droplet formation system and method. United States.
Van den Engh, Ger. Sat . "High speed flow cytometer droplet formation system and method". United States. https://www.osti.gov/servlets/purl/873315.
@article{osti_873315,
title = {High speed flow cytometer droplet formation system and method},
author = {Van den Engh, Ger},
abstractNote = {A droplet forming flow cytometer system allows high speed processing without the need for high oscillator drive powers through the inclusion of an oscillator or piezoelectric crystal such as within the nozzle volume or otherwise unidirectionally coupled to the sheath fluid. The nozzle container continuously converges so as to amplify unidirectional oscillations which are transmitted as pressure waves through the nozzle volume to the nozzle exit so as to form droplets from the fluid jet. The oscillator is directionally isolated so as to avoid moving the entire nozzle container so as to create only pressure waves within the sheath fluid. A variation in substance concentration is achieved through a movable substance introduction port which is positioned within a convergence zone to vary the relative concentration of substance to sheath fluid while still maintaining optimal laminar flow conditions. This variation may be automatically controlled through a sensor and controller configuration. A replaceable tip design is also provided whereby the ceramic nozzle tip is positioned within an edge insert in the nozzle body so as to smoothly transition from nozzle body to nozzle tip. The nozzle tip is sealed against its outer surface to the nozzle body so it may be removable for cleaning or replacement.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {1}
}

Patent:

Save / Share: