High speed flow cytometer droplet formation system and method
Abstract
A droplet forming flow cytometer system allows high speed processing without the need for high oscillator drive powers through the inclusion of an oscillator or piezoelectric crystal such as within the nozzle volume or otherwise unidirectionally coupled to the sheath fluid. The nozzle container continuously converges so as to amplify unidirectional oscillations which are transmitted as pressure waves through the nozzle volume to the nozzle exit so as to form droplets from the fluid jet. The oscillator is directionally isolated so as to avoid moving the entire nozzle container so as to create only pressure waves within the sheath fluid. A variation in substance concentration is achieved through a movable substance introduction port which is positioned within a convergence zone to vary the relative concentration of substance to sheath fluid while still maintaining optimal laminar flow conditions. This variation may be automatically controlled through a sensor and controller configuration. A replaceable tip design is also provided whereby the ceramic nozzle tip is positioned within an edge insert in the nozzle body so as to smoothly transition from nozzle body to nozzle tip. The nozzle tip is sealed against its outer surface to the nozzle body so it may be removablemore »
- Inventors:
-
- Seattle, WA
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- OSTI Identifier:
- 873315
- Patent Number(s):
- 6133044
- Assignee:
- University of Washington (Seattle, WA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- DOE Contract Number:
- FG06-93ER61662
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- speed; flow; cytometer; droplet; formation; method; forming; allows; processing; oscillator; drive; powers; inclusion; piezoelectric; crystal; nozzle; volume; otherwise; unidirectionally; coupled; sheath; fluid; container; continuously; converges; amplify; unidirectional; oscillations; transmitted; pressure; waves; exit; form; droplets; jet; directionally; isolated; avoid; moving; entire; create; variation; substance; concentration; achieved; movable; introduction; positioned; convergence; zone; vary; relative; maintaining; optimal; laminar; conditions; automatically; controlled; sensor; controller; configuration; replaceable; tip; design; provided; whereby; ceramic; edge; insert; smoothly; transition; sealed; outer; surface; removable; cleaning; replacement; fluid jet; droplet formation; nozzle tip; nozzle exit; pressure waves; provided whereby; outer surface; flow cytometer; laminar flow; sheath fluid; piezoelectric crystal; flow conditions; pressure wave; automatically control; relative concentration; nozzle container; smoothly transition; ceramic nozzle; form droplets; speed flow; /436/209/324/356/422/
Citation Formats
Van den Engh, Ger. High speed flow cytometer droplet formation system and method. United States: N. p., 2000.
Web.
Van den Engh, Ger. High speed flow cytometer droplet formation system and method. United States.
Van den Engh, Ger. Sat .
"High speed flow cytometer droplet formation system and method". United States. https://www.osti.gov/servlets/purl/873315.
@article{osti_873315,
title = {High speed flow cytometer droplet formation system and method},
author = {Van den Engh, Ger},
abstractNote = {A droplet forming flow cytometer system allows high speed processing without the need for high oscillator drive powers through the inclusion of an oscillator or piezoelectric crystal such as within the nozzle volume or otherwise unidirectionally coupled to the sheath fluid. The nozzle container continuously converges so as to amplify unidirectional oscillations which are transmitted as pressure waves through the nozzle volume to the nozzle exit so as to form droplets from the fluid jet. The oscillator is directionally isolated so as to avoid moving the entire nozzle container so as to create only pressure waves within the sheath fluid. A variation in substance concentration is achieved through a movable substance introduction port which is positioned within a convergence zone to vary the relative concentration of substance to sheath fluid while still maintaining optimal laminar flow conditions. This variation may be automatically controlled through a sensor and controller configuration. A replaceable tip design is also provided whereby the ceramic nozzle tip is positioned within an edge insert in the nozzle body so as to smoothly transition from nozzle body to nozzle tip. The nozzle tip is sealed against its outer surface to the nozzle body so it may be removable for cleaning or replacement.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2000},
month = {Sat Jan 01 00:00:00 EST 2000}
}
Works referenced in this record:
Operation of a Flow Cytometer
book, January 1992
- Göttlinger, C.; Mechtold, B.; Radbruch, A.
- Flow Cytometry and Cell Sorting