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Title: Methods of biological dosimetry employing chromosome-specific staining

Abstract

Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods are provided to disable the hybridization capacity of shared, high copy repetitive sequences and/or remove such sequences to provide for useful contrast. Still further methods are provided to produce chromosome-specific staining reagents which are made specific to the targeted chromosomal material, which can be one or more whole chromosomes, one or more regions on one or more chromosomes, subsets of chromosomes and/or the entire genome. Probes and test kits are provided for use in tumor cytogenetics, in the detection of disease related loci, in analysis of structural abnormalities, such as translocations, and for biological dosimetry. Further, methods and prenatal test kits are provided to stain targeted chromosomal material of fetal cells, including fetal cells obtained from maternal blood. Still further, the invention provides for automated means to detect and analysemore » chromosomal abnormalities.« less

Inventors:
 [1];  [2]
  1. (Livermore, CA)
  2. (Walnut Creek, CA)
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
873313
Patent Number(s):
6132961
Assignee:
Regents of University of California (Oakland, CA) LLNL
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
methods; biological; dosimetry; employing; chromosome-specific; staining; compositions; based; nucleic; acid; sequence; employ; probes; provided; produce; patterns; tailored; specific; cytogenetic; analyses; appropriate; situ; hybridization; stain; interphase; metaphase; chromosomal; material; reliable; signals; typically; complexity; 50; kb; depending; application; disable; capacity; shared; copy; repetitive; sequences; remove; provide; useful; contrast; reagents; targeted; chromosomes; regions; subsets; entire; genome; kits; tumor; cytogenetics; detection; disease; related; loci; analysis; structural; abnormalities; translocations; prenatal; fetal; cells; including; obtained; maternal; blood; provides; automated; means; detect; analyse; chromosomal abnormalities; acid probes; acid sequence; nucleic acid; chromosomal material; acid probe; chromosome-specific staining; situ hybridization; reliable signals; repetitive sequences; specific cytogenetic; staining based; staining patterns; related loci; methods produce; tumor cytogenetics; produce staining; metaphase chromosomal; complexity depending; biological dosimetry; staining reagent; employ nucleic; cytogenetic analyses; cytogenetic application; disease related; /435/422/

Citation Formats

Gray, Joe W., and Pinkel, Daniel. Methods of biological dosimetry employing chromosome-specific staining. United States: N. p., 2000. Web.
Gray, Joe W., & Pinkel, Daniel. Methods of biological dosimetry employing chromosome-specific staining. United States.
Gray, Joe W., and Pinkel, Daniel. Sat . "Methods of biological dosimetry employing chromosome-specific staining". United States. https://www.osti.gov/servlets/purl/873313.
@article{osti_873313,
title = {Methods of biological dosimetry employing chromosome-specific staining},
author = {Gray, Joe W. and Pinkel, Daniel},
abstractNote = {Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods are provided to disable the hybridization capacity of shared, high copy repetitive sequences and/or remove such sequences to provide for useful contrast. Still further methods are provided to produce chromosome-specific staining reagents which are made specific to the targeted chromosomal material, which can be one or more whole chromosomes, one or more regions on one or more chromosomes, subsets of chromosomes and/or the entire genome. Probes and test kits are provided for use in tumor cytogenetics, in the detection of disease related loci, in analysis of structural abnormalities, such as translocations, and for biological dosimetry. Further, methods and prenatal test kits are provided to stain targeted chromosomal material of fetal cells, including fetal cells obtained from maternal blood. Still further, the invention provides for automated means to detect and analyse chromosomal abnormalities.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {1}
}

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