Integrated polymerase chain reaction/electrophoresis instrument
Abstract
A new approach and instrument for field identification of micro-organisms and DNA fragments using a small and disposable device containing integrated polymerase chain reaction (PCR) enzymatic reaction wells, attached capillary electrophoresis (CE) channels, detectors, and read-out all on/in a small hand-held package. The analysis instrument may be made inexpensively, for example, of plastic, and thus is disposable, which minimizes cross contamination and the potential for false positive identification between samples. In addition, it is designed for multiple users with individual applications. The integrated PCR/CE is manufactured by the PCR well and CE channels are "stamped" into plastic depressions where conductive coatings are made in the wells and ends of the CE microchannels to carry voltage and current to heat the PCR reaction mixtures and simultaneously draw DNA bands up the CE channels. Light is transmitted through the instrument at appropriate points and detects PCR bands and identifies DNA fragments by size (retention time) and quantifies each by the amount of light generated as each phototransistor positioned below each CE channel detects a passing band. The instrument is so compact that at least 100 PCR/CE reactions/analyses can be performed easily on one detection device.
- Inventors:
-
- Livermore, CA
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- OSTI Identifier:
- 873279
- Patent Number(s):
- 6126804
- Assignee:
- Regents of University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- integrated; polymerase; chain; reaction; electrophoresis; instrument; approach; field; identification; micro-organisms; dna; fragments; disposable; device; containing; pcr; enzymatic; attached; capillary; channels; detectors; read-out; hand-held; package; analysis; inexpensively; example; plastic; minimizes; contamination; potential; false; positive; samples; addition; designed; multiple; users; individual; applications; manufactured; stamped; depressions; conductive; coatings; microchannels; carry; voltage; current; heat; mixtures; simultaneously; draw; bands; light; transmitted; appropriate; detects; identifies; size; retention; time; quantifies; amount; generated; phototransistor; positioned; below; channel; passing; band; compact; 100; reactions; analyses; performed; easily; detection; enzymatic reaction; positioned below; device containing; detection device; chain reaction; capillary electrophoresis; reaction mixture; dna fragments; polymerase chain; light generated; dna fragment; positive identification; retention time; multiple users; conductive coatings; integrated polymerase; analysis instrument; conductive coating; /204/435/
Citation Formats
Andresen, Brian D. Integrated polymerase chain reaction/electrophoresis instrument. United States: N. p., 2000.
Web.
Andresen, Brian D. Integrated polymerase chain reaction/electrophoresis instrument. United States.
Andresen, Brian D. Sat .
"Integrated polymerase chain reaction/electrophoresis instrument". United States. https://www.osti.gov/servlets/purl/873279.
@article{osti_873279,
title = {Integrated polymerase chain reaction/electrophoresis instrument},
author = {Andresen, Brian D},
abstractNote = {A new approach and instrument for field identification of micro-organisms and DNA fragments using a small and disposable device containing integrated polymerase chain reaction (PCR) enzymatic reaction wells, attached capillary electrophoresis (CE) channels, detectors, and read-out all on/in a small hand-held package. The analysis instrument may be made inexpensively, for example, of plastic, and thus is disposable, which minimizes cross contamination and the potential for false positive identification between samples. In addition, it is designed for multiple users with individual applications. The integrated PCR/CE is manufactured by the PCR well and CE channels are "stamped" into plastic depressions where conductive coatings are made in the wells and ends of the CE microchannels to carry voltage and current to heat the PCR reaction mixtures and simultaneously draw DNA bands up the CE channels. Light is transmitted through the instrument at appropriate points and detects PCR bands and identifies DNA fragments by size (retention time) and quantifies each by the amount of light generated as each phototransistor positioned below each CE channel detects a passing band. The instrument is so compact that at least 100 PCR/CE reactions/analyses can be performed easily on one detection device.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2000},
month = {Sat Jan 01 00:00:00 EST 2000}
}
Works referenced in this record:
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