Multiple protocol fluorometer and method
Abstract
A multiple protocol fluorometer measures photosynthetic parameters of phytoplankton and higher plants using actively stimulated fluorescence protocols. The measured parameters include spectrally-resolved functional and optical absorption cross sections of PSII, extent of energy transfer between reaction centers of PSII, F.sub.0 (minimal), F.sub.m (maximal) and F.sub.v (variable) components of PSII fluorescence, photochemical and non-photochemical quenching, size of the plastoquinone (PQ) pool, and the kinetics of electron transport between Q.sub.a and PQ pool and between PQ pool and PSI. The multiple protocol fluorometer, in one embodiment, is equipped with an excitation source having a controlled spectral output range between 420 nm and 555 nm and capable of generating flashlets having a duration of 0.125-32 .mu.s, an interval between 0.5 .mu.s and 2 seconds, and peak optical power of up to 2 W/cm.sup.2. The excitation source is also capable of generating, simultaneous with the flashlets, a controlled continuous, background illumination.
- Inventors:
-
- Shoreham, NY
- Stony Brook, NY
- Issue Date:
- Research Org.:
- Associated Universities, Inc., Upton, NY (United States)
- OSTI Identifier:
- 873253
- Patent Number(s):
- 6121053
- Application Number:
- 08/988,269
- Assignee:
- Brookhaven Science Associates (Upton, NY)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- AC02-76CH00016
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- multiple; protocol; fluorometer; method; measures; photosynthetic; parameters; phytoplankton; plants; actively; stimulated; fluorescence; protocols; measured; spectrally-resolved; functional; optical; absorption; sections; psii; extent; energy; transfer; reaction; centers; minimal; maximal; variable; components; photochemical; non-photochemical; quenching; size; plastoquinone; pq; pool; kinetics; electron; transport; psi; embodiment; equipped; excitation; source; controlled; spectral; output; range; 420; nm; 555; capable; generating; flashlets; duration; 125-32; interval; peak; power; cm; simultaneous; continuous; background; illumination; excitation source; optical absorption; energy transfer; electron transport; optical power; protocol fluorometer; photosynthetic parameters; multiple protocol; measured parameters; meter measures; measured parameter; controlled spectral; /436/250/422/
Citation Formats
Kolber, Zbigniew S, and Falkowski, Paul G. Multiple protocol fluorometer and method. United States: N. p., 2000.
Web.
Kolber, Zbigniew S, & Falkowski, Paul G. Multiple protocol fluorometer and method. United States.
Kolber, Zbigniew S, and Falkowski, Paul G. Tue .
"Multiple protocol fluorometer and method". United States. https://www.osti.gov/servlets/purl/873253.
@article{osti_873253,
title = {Multiple protocol fluorometer and method},
author = {Kolber, Zbigniew S and Falkowski, Paul G},
abstractNote = {A multiple protocol fluorometer measures photosynthetic parameters of phytoplankton and higher plants using actively stimulated fluorescence protocols. The measured parameters include spectrally-resolved functional and optical absorption cross sections of PSII, extent of energy transfer between reaction centers of PSII, F.sub.0 (minimal), F.sub.m (maximal) and F.sub.v (variable) components of PSII fluorescence, photochemical and non-photochemical quenching, size of the plastoquinone (PQ) pool, and the kinetics of electron transport between Q.sub.a and PQ pool and between PQ pool and PSI. The multiple protocol fluorometer, in one embodiment, is equipped with an excitation source having a controlled spectral output range between 420 nm and 555 nm and capable of generating flashlets having a duration of 0.125-32 .mu.s, an interval between 0.5 .mu.s and 2 seconds, and peak optical power of up to 2 W/cm.sup.2. The excitation source is also capable of generating, simultaneous with the flashlets, a controlled continuous, background illumination.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {9}
}