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Title: Multiple protocol fluorometer and method

Abstract

A multiple protocol fluorometer measures photosynthetic parameters of phytoplankton and higher plants using actively stimulated fluorescence protocols. The measured parameters include spectrally-resolved functional and optical absorption cross sections of PSII, extent of energy transfer between reaction centers of PSII, F.sub.0 (minimal), F.sub.m (maximal) and F.sub.v (variable) components of PSII fluorescence, photochemical and non-photochemical quenching, size of the plastoquinone (PQ) pool, and the kinetics of electron transport between Q.sub.a and PQ pool and between PQ pool and PSI. The multiple protocol fluorometer, in one embodiment, is equipped with an excitation source having a controlled spectral output range between 420 nm and 555 nm and capable of generating flashlets having a duration of 0.125-32 .mu.s, an interval between 0.5 .mu.s and 2 seconds, and peak optical power of up to 2 W/cm.sup.2. The excitation source is also capable of generating, simultaneous with the flashlets, a controlled continuous, background illumination.

Inventors:
 [1];  [2]
  1. (Shoreham, NY)
  2. (Stony Brook, NY)
Issue Date:
Research Org.:
ASSOC UNIVERSITIES INC
OSTI Identifier:
873253
Patent Number(s):
6121053
Application Number:
08/988,269
Assignee:
Brookhaven Science Associates (Upton, NY) BNL
DOE Contract Number:  
AC02-76CH00016
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
multiple; protocol; fluorometer; method; measures; photosynthetic; parameters; phytoplankton; plants; actively; stimulated; fluorescence; protocols; measured; spectrally-resolved; functional; optical; absorption; sections; psii; extent; energy; transfer; reaction; centers; minimal; maximal; variable; components; photochemical; non-photochemical; quenching; size; plastoquinone; pq; pool; kinetics; electron; transport; psi; embodiment; equipped; excitation; source; controlled; spectral; output; range; 420; nm; 555; capable; generating; flashlets; duration; 125-32; interval; peak; power; cm; simultaneous; continuous; background; illumination; excitation source; optical absorption; energy transfer; electron transport; optical power; protocol fluorometer; photosynthetic parameters; multiple protocol; measured parameters; meter measures; measured parameter; controlled spectral; /436/250/422/

Citation Formats

Kolber, Zbigniew S., and Falkowski, Paul G. Multiple protocol fluorometer and method. United States: N. p., 2000. Web.
Kolber, Zbigniew S., & Falkowski, Paul G. Multiple protocol fluorometer and method. United States.
Kolber, Zbigniew S., and Falkowski, Paul G. Tue . "Multiple protocol fluorometer and method". United States. https://www.osti.gov/servlets/purl/873253.
@article{osti_873253,
title = {Multiple protocol fluorometer and method},
author = {Kolber, Zbigniew S. and Falkowski, Paul G.},
abstractNote = {A multiple protocol fluorometer measures photosynthetic parameters of phytoplankton and higher plants using actively stimulated fluorescence protocols. The measured parameters include spectrally-resolved functional and optical absorption cross sections of PSII, extent of energy transfer between reaction centers of PSII, F.sub.0 (minimal), F.sub.m (maximal) and F.sub.v (variable) components of PSII fluorescence, photochemical and non-photochemical quenching, size of the plastoquinone (PQ) pool, and the kinetics of electron transport between Q.sub.a and PQ pool and between PQ pool and PSI. The multiple protocol fluorometer, in one embodiment, is equipped with an excitation source having a controlled spectral output range between 420 nm and 555 nm and capable of generating flashlets having a duration of 0.125-32 .mu.s, an interval between 0.5 .mu.s and 2 seconds, and peak optical power of up to 2 W/cm.sup.2. The excitation source is also capable of generating, simultaneous with the flashlets, a controlled continuous, background illumination.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {9}
}

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