Microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same
Abstract
A microfabricated device and method for electrokinetic transport of a liquid phase biological or chemical material is described. In accordance with one aspect of the present invention there is provided a microchip that is adapted for the simultaneous spatial confinement of electrokinetically driven fluidic material streams on a substrate. The apparatus includes a focusing chamber formed in a surface of the substrate and in fluid communication with two sample fluid channels and three focusing fluid channels. The device further includes electromotive means operatively connected to the sources of the sample fluid and the source of focusing fluid for electrokinetically driving the respective streams of the sample and focusing fluids through the respective channels into the focusing chamber such that the focusing fluid streams spatially confine the first and second sample fluid streams within the focusing chamber. In accordance with another aspect of this invention, there is provided a cytometry method for analyzing microscopic particles in a fluid medium on a microchip by utilizing the focusing function of the microchip. In the disclosed cytometry process the width of the fluid stream is narrowed in the focusing chamber. The microscopic particles in the focused sample fluid are then detected and/or measured usingmore »
- Inventors:
-
- Knoxville, TN
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- OSTI Identifier:
- 873247
- Patent Number(s):
- 6120666
- Assignee:
- UT-Battelle, LLC (Oak Ridge, TN)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- AC05-96OR22464
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- microfabricated; device; method; multiplexed; electrokinetic; focusing; fluid; streams; transport; cytometry; liquid; phase; biological; chemical; material; described; accordance; aspect; provided; microchip; adapted; simultaneous; spatial; confinement; electrokinetically; driven; fluidic; substrate; apparatus; chamber; formed; surface; communication; sample; channels; electromotive; means; operatively; connected; sources; source; driving; respective; fluids; spatially; confine; analyzing; microscopic; particles; medium; utilizing; function; disclosed; process; width; stream; narrowed; focused; detected; measured; light; scattering; techniques; microfabricated device; fluid streams; microscopic particles; light scattering; fluid stream; liquid phase; fluid communication; sample fluid; operatively connected; fluid medium; chamber formed; fluid channels; chemical material; electrokinetic transport; means operative; analyzing micro; /204/137/422/435/
Citation Formats
Jacobson, Stephen C, and Ramsey, J Michael. Microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same. United States: N. p., 2000.
Web.
Jacobson, Stephen C, & Ramsey, J Michael. Microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same. United States.
Jacobson, Stephen C, and Ramsey, J Michael. Sat .
"Microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same". United States. https://www.osti.gov/servlets/purl/873247.
@article{osti_873247,
title = {Microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same},
author = {Jacobson, Stephen C and Ramsey, J Michael},
abstractNote = {A microfabricated device and method for electrokinetic transport of a liquid phase biological or chemical material is described. In accordance with one aspect of the present invention there is provided a microchip that is adapted for the simultaneous spatial confinement of electrokinetically driven fluidic material streams on a substrate. The apparatus includes a focusing chamber formed in a surface of the substrate and in fluid communication with two sample fluid channels and three focusing fluid channels. The device further includes electromotive means operatively connected to the sources of the sample fluid and the source of focusing fluid for electrokinetically driving the respective streams of the sample and focusing fluids through the respective channels into the focusing chamber such that the focusing fluid streams spatially confine the first and second sample fluid streams within the focusing chamber. In accordance with another aspect of this invention, there is provided a cytometry method for analyzing microscopic particles in a fluid medium on a microchip by utilizing the focusing function of the microchip. In the disclosed cytometry process the width of the fluid stream is narrowed in the focusing chamber. The microscopic particles in the focused sample fluid are then detected and/or measured using light scattering or other techniques.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2000},
month = {Sat Jan 01 00:00:00 EST 2000}
}