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Title: Reversible gelling culture media for in-vitro cell culture in three-dimensional matrices

Abstract

A gelling cell culture medium useful for forming a three dimensional matrix for cell culture in vitro is prepared by copolymerizing an acrylamide derivative with a hydrophilic comonomer to form a reversible (preferably thermally reversible) gelling linear random copolymer in the form of a plurality of linear chains having a plurality of molecular weights greater than or equal to a minimum gelling molecular weight cutoff, mixing the copolymer with an aqueous solvent to form a reversible gelling solution and adding a cell culture medium to the gelling solution to form the gelling cell culture medium. Cells such as chondrocytes or hepatocytes are added to the culture medium to form a seeded culture medium, and temperature of the medium is raised to gel the seeded culture medium and form a three dimensional matrix containing the cells. After propagating the cells in the matrix, the cells may be recovered by lowering the temperature to dissolve the matrix and centrifuging.

Inventors:
 [1];  [2];  [3]
  1. Charleston, SC
  2. Mt. Pleasant, SC
  3. Richland, WA
Issue Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
OSTI Identifier:
873161
Patent Number(s):
6103528
Assignee:
Battelle Memorial Institute (Richland, WA); Medical University of South Carolina (Charleston, SC)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
reversible; gelling; culture; media; in-vitro; cell; three-dimensional; matrices; medium; useful; forming; dimensional; matrix; vitro; prepared; copolymerizing; acrylamide; derivative; hydrophilic; comonomer; form; preferably; thermally; linear; random; copolymer; plurality; chains; molecular; weights; equal; minimum; weight; cutoff; mixing; aqueous; solvent; solution; adding; cells; chondrocytes; hepatocytes; added; seeded; temperature; raised; containing; propagating; recovered; lowering; dissolve; centrifuging; reversible gelling; random copolymer; aqueous solvent; culture medium; molecular weight; cell culture; molecular weights; thermally reversible; dimensional matrix; matrix containing; linear random; vitro cell; weight cu; acrylamide derivative; /435/424/

Citation Formats

An, Yuehuei H, Mironov, Vladimir A, and Gutowska, Anna. Reversible gelling culture media for in-vitro cell culture in three-dimensional matrices. United States: N. p., 2000. Web.
An, Yuehuei H, Mironov, Vladimir A, & Gutowska, Anna. Reversible gelling culture media for in-vitro cell culture in three-dimensional matrices. United States.
An, Yuehuei H, Mironov, Vladimir A, and Gutowska, Anna. Sat . "Reversible gelling culture media for in-vitro cell culture in three-dimensional matrices". United States. https://www.osti.gov/servlets/purl/873161.
@article{osti_873161,
title = {Reversible gelling culture media for in-vitro cell culture in three-dimensional matrices},
author = {An, Yuehuei H and Mironov, Vladimir A and Gutowska, Anna},
abstractNote = {A gelling cell culture medium useful for forming a three dimensional matrix for cell culture in vitro is prepared by copolymerizing an acrylamide derivative with a hydrophilic comonomer to form a reversible (preferably thermally reversible) gelling linear random copolymer in the form of a plurality of linear chains having a plurality of molecular weights greater than or equal to a minimum gelling molecular weight cutoff, mixing the copolymer with an aqueous solvent to form a reversible gelling solution and adding a cell culture medium to the gelling solution to form the gelling cell culture medium. Cells such as chondrocytes or hepatocytes are added to the culture medium to form a seeded culture medium, and temperature of the medium is raised to gel the seeded culture medium and form a three dimensional matrix containing the cells. After propagating the cells in the matrix, the cells may be recovered by lowering the temperature to dissolve the matrix and centrifuging.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {1}
}

Works referenced in this record:

Thermally reversible polymer gels for biohybrid artificial pancreas
journal, May 1996


Fibroblast growth factor stimulates colony formation of differentiated chondrocytes in soft agar
journal, December 1987


Treatment of Deep Cartilage Defects in the Knee with Autologous Chondrocyte Transplantation
journal, October 1994


Develoapmet of a Tissue Analog for Cartilage Repair
journal, January 1991


Enhanced Tumor Growth of Both Primary and Established Human and Murine Tumor Cells in Athymic Mice After Coinjection With Matrigel
journal, June 1991