Method for detecting and diagnosing disease caused by pathological protein aggregation
Abstract
A method is provided for detecting pathological macromolecules in a patient, comprising obtaining body fluid from the patient, pretreating the body fluid, subjecting the pretreated body fluid to size-exclusion chromatography to create an excluded fluid, and analyzing the excluded fluid to detect macromolecules having a predetermined molecular weight. The method also allows for comparing elution spectra with reference spectra of suspect pathologic proteins.
- Inventors:
-
- Naperville, IL
- Riverside, IL
- Knoxville, TN
- Issue Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- OSTI Identifier:
- 872999
- Patent Number(s):
- 6063636
- Assignee:
- United States of America as represented by United States (Washington, DC); University of Tennessee (Knoxville, TN)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01D - SEPARATION
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- W-31109-ENG-38
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- method; detecting; diagnosing; disease; caused; pathological; protein; aggregation; provided; macromolecules; patient; comprising; obtaining; fluid; pretreating; subjecting; pretreated; size-exclusion; chromatography; create; excluded; analyzing; detect; predetermined; molecular; weight; allows; comparing; elution; spectra; reference; suspect; pathologic; proteins; molecular weight; size-exclusion chromatography; disease caused; /436/530/
Citation Formats
Stevens, Fred J, Myatt, Elizabeth A, and Solomon, Alan. Method for detecting and diagnosing disease caused by pathological protein aggregation. United States: N. p., 2000.
Web.
Stevens, Fred J, Myatt, Elizabeth A, & Solomon, Alan. Method for detecting and diagnosing disease caused by pathological protein aggregation. United States.
Stevens, Fred J, Myatt, Elizabeth A, and Solomon, Alan. Sat .
"Method for detecting and diagnosing disease caused by pathological protein aggregation". United States. https://www.osti.gov/servlets/purl/872999.
@article{osti_872999,
title = {Method for detecting and diagnosing disease caused by pathological protein aggregation},
author = {Stevens, Fred J and Myatt, Elizabeth A and Solomon, Alan},
abstractNote = {A method is provided for detecting pathological macromolecules in a patient, comprising obtaining body fluid from the patient, pretreating the body fluid, subjecting the pretreated body fluid to size-exclusion chromatography to create an excluded fluid, and analyzing the excluded fluid to detect macromolecules having a predetermined molecular weight. The method also allows for comparing elution spectra with reference spectra of suspect pathologic proteins.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {1}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Polymeric Forms of Free Normal κ and λ Chains of Human Immunoglobulin
journal, August 1969
- Berggard, I.; Peterson, P. A.
- Journal of Biological Chemistry, Vol. 244, Issue 16
Analysis of protein-protein interaction by simulation of small-zone size exclusion chromatography. Stochastic formulation of kinetic rate contributions to observed high-performance liquid chromatography elution characteristics
journal, June 1989
- Stevens, F. J.
- Biophysical Journal, Vol. 55, Issue 6