Dyes designed for high sensitivity detection of double-stranded DNA
Abstract
Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a cationic chain. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.
- Inventors:
-
- Orinda, CA
- Oakland, CA
- Issue Date:
- Research Org.:
- Univ. of California (United States)
- OSTI Identifier:
- 872967
- Patent Number(s):
- 6054272
- Assignee:
- Regents of University of California (Berkeley, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07D - HETEROCYCLIC COMPOUNDS
C - CHEMISTRY C09 - DYES C09B - ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES {, e.g. PIGMENTS},
- DOE Contract Number:
- FG03-91ER61125
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- dyes; designed; sensitivity; detection; double-stranded; dna; novel; fluorescent; provided; characterized; fluorophore; joined; cationic; chain; found; provide; enhancement; binding; nucleic; acid; strong; affinities; compared; polycationic; dsdna; electrophoresis; solution; substantially; sensitivities; dye; novel fluorescent; stranded dna; dyes designed; double-stranded dna; nucleic acid; fluorescent dyes; fluorescent dye; sensitivity detection; strong binding; binding affinities; polycationic chain; cationic chain; fluorophore joined; /435/999/
Citation Formats
Glazer, Alexander N, and Benson, Scott C. Dyes designed for high sensitivity detection of double-stranded DNA. United States: N. p., 2000.
Web.
Glazer, Alexander N, & Benson, Scott C. Dyes designed for high sensitivity detection of double-stranded DNA. United States.
Glazer, Alexander N, and Benson, Scott C. Sat .
"Dyes designed for high sensitivity detection of double-stranded DNA". United States. https://www.osti.gov/servlets/purl/872967.
@article{osti_872967,
title = {Dyes designed for high sensitivity detection of double-stranded DNA},
author = {Glazer, Alexander N and Benson, Scott C},
abstractNote = {Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a cationic chain. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2000},
month = {Sat Jan 01 00:00:00 EST 2000}
}
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