In vitro gastrointestinal mimetic protocol for measuring bioavailable contaminants
Abstract
The present invention relates to measurements of contaminants in the soil and other organic or environmental materials, using a biologically relevant chemical analysis that will measure the amount of contaminants in a given sample that may be expected to be absorbed by a human being ingesting the contaminated soil. According to the present invention, environmental samples to be tested are added to a pre-prepared physiological composition of bile salts and lipids. They are thoroughly mixed and then the resulting mixture is separated e.g. by centrifugation. The supernatant is then analyzed for the presence of contaminants and these concentrations are compared to the level of contaminants in the untreated samples. It is important that the bile salts and lipids be thoroughly pre-mixed to form micelles.
- Inventors:
-
- Berkeley, CA
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- OSTI Identifier:
- 872923
- Patent Number(s):
- 6040188
- Assignee:
- Regents of University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- DOE Contract Number:
- AC03-76SF00098
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- vitro; gastrointestinal; mimetic; protocol; measuring; bioavailable; contaminants; relates; measurements; soil; organic; environmental; materials; biologically; relevant; chemical; analysis; measure; amount; sample; expected; absorbed; human; ingesting; contaminated; according; samples; tested; added; pre-prepared; physiological; composition; bile; salts; lipids; thoroughly; mixed; resulting; mixture; separated; centrifugation; supernatant; analyzed; presence; concentrations; compared; level; untreated; pre-mixed; form; micelles; environmental sample; contaminated soil; chemical analysis; resulting mixture; environmental samples; treated sample; thoroughly mix; /436/
Citation Formats
Holman, Hoi-Ying N. In vitro gastrointestinal mimetic protocol for measuring bioavailable contaminants. United States: N. p., 2000.
Web.
Holman, Hoi-Ying N. In vitro gastrointestinal mimetic protocol for measuring bioavailable contaminants. United States.
Holman, Hoi-Ying N. Sat .
"In vitro gastrointestinal mimetic protocol for measuring bioavailable contaminants". United States. https://www.osti.gov/servlets/purl/872923.
@article{osti_872923,
title = {In vitro gastrointestinal mimetic protocol for measuring bioavailable contaminants},
author = {Holman, Hoi-Ying N},
abstractNote = {The present invention relates to measurements of contaminants in the soil and other organic or environmental materials, using a biologically relevant chemical analysis that will measure the amount of contaminants in a given sample that may be expected to be absorbed by a human being ingesting the contaminated soil. According to the present invention, environmental samples to be tested are added to a pre-prepared physiological composition of bile salts and lipids. They are thoroughly mixed and then the resulting mixture is separated e.g. by centrifugation. The supernatant is then analyzed for the presence of contaminants and these concentrations are compared to the level of contaminants in the untreated samples. It is important that the bile salts and lipids be thoroughly pre-mixed to form micelles.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {1}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Soil adsorption alters kinetics and bioavailability of benzene in orally exposed male rats
journal, March 1988
- Turkall, Rita M.; Skowronski, Gloria; Gerges, Samy
- Archives of Environmental Contamination and Toxicology, Vol. 17, Issue 2
A Multicompartmental Dynamic Computer-controlled Model Simulating the Stomach and Small Intestine
journal, March 1995
- Minekus, Mans; Marteau, Phillipe; Havenaar, Robert
- Alternatives to Laboratory Animals, Vol. 23, Issue 2
Modulating effects in human diets of dietary fibre and beef, and of time and dose on the reactive microcapsule trapping of benzo[ a ]pyrene matabolites in the rat gastrointestinal tract
journal, January 1990
- O'Neill, I. K.; Bingham, S.; Povey, A. C.
- Carcinogenesis, Vol. 11, Issue 4
Development of an in vitro screening test to evaluate the in vivo bioaccessibility of ingested mine-waste lead
journal, December 1993
- Ruby, Michael V.; Davis, Andy; Link, Timothy E.
- Environmental Science & Technology, Vol. 27, Issue 13
Estimation of Lead and Arsenic Bioavailability Using a Physiologically Based Extraction Test
journal, January 1996
- Ruby, Michael V.; Davis, Andy; Schoof, Rosalind
- Environmental Science & Technology, Vol. 30, Issue 2
In vitro simulation of gastric digestion of milk proteins: comparison between in vitro and in vivo data
journal, February 1992
- Yvon, Mireille.; Beucher, Sylvie.; Scanff, Pascale.
- Journal of Agricultural and Food Chemistry, Vol. 40, Issue 2
Physical-chemical behavior of dietary and biliary lipids during intestinal digestion and absorption. 2. Phase analysis and aggregation states of luminal lipids during duodenal fat digestion in healthy adult human beings
journal, February 1990
- Hernell, Olle; Staggers, Joan E.; Carey, Martin C.
- Biochemistry, Vol. 29, Issue 8
Micromineralogy of mine wastes in relation to lead bioavailability, Butte, Montana
journal, July 1993
- Davis, Andy; Drexler, John W.; Ruby, Michael V.
- Environmental Science & Technology, Vol. 27, Issue 7
Estimation of Agitation Intensity in the GI Tract in Humans and Dogs Based on in Vitro/in Vivo Correlation
journal, January 1995
- Katori, Noriko; Aoyagi, Nobuo; Terao, Tadao
- Pharmaceutical Research, Vol. 12, Issue 2, p. 237-243
Physical-chemical behavior of dietary and biliary lipids during intestinal digestion and absorption. 1. Phase behavior and aggregation states of model lipid systems patterned after aqueous duodenal contents of healthy adult human beings
journal, February 1990
- Staggers, Joan E.; Hernell, Olle; Stafford, Richard J.
- Biochemistry, Vol. 29, Issue 8