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Title: Isolation and bacterial expression of a sesquiterpene synthase CDNA clone from peppermint(mentha .chi. piperita, L.) that produces the aphid alarm pheromone (E)-.beta.-farnesene

Abstract

A cDNA encoding (E)-.beta.-farnesene synthase from peppermint (Mentha piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of (E)-.beta.-farnesene synthase (SEQ ID NO:2), from peppermint (Mentha piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for (E)-.beta.-farnesene synthase, or for a base sequence sufficiently complementary to at least a portion of (E)-.beta.-farnesene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (E)-.beta.-farnesene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant (E)-.beta.-farnesene synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant (E)-.beta.-farnesene synthase may be used to obtain expression or enhanced expression of (E)-.beta.-farnesene synthase in plants in order to enhance the production of (E)-.beta.-farnesene, or may be otherwise employed for the regulation or expression of (E)-.beta.-farnesene synthase, or the production of its product.

Inventors:
 [1];  [2];  [3]
  1. Pullman, WA
  2. Colfax, WA
  3. Moscow, ID
Issue Date:
Research Org.:
Washington State University Research Foundation (Pullman, WA)
OSTI Identifier:
872786
Patent Number(s):
6008043
Assignee:
Washington State University Research Foundation (Pullman, WA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG03-96ER20212
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
isolation; bacterial; expression; sesquiterpene; synthase; cdna; clone; peppermint; mentha; chi; piperita; produces; aphid; alarm; pheromone; beta; -farnesene; encoding; isolated; sequenced; corresponding; amino; acid; sequence; determined; accordingly; dna; seq; provided; codes; aspects; replicable; recombinant; cloning; vehicles; code; base; sufficiently; complementary; portion; rna; enable; hybridization; therewith; modified; host; cells; transformed; transfected; infected; injected; vehicle; systems; methods; aforementioned; facilitate; production; purification; significant; amounts; obtain; enhanced; plants; enhance; otherwise; employed; regulation; product; sequence sufficiently; modified host; cloning vehicles; cdna clone; recombinant expression; replicable recombinant; obtain expression; otherwise employed; enable hybridization; hybridization therewith; synthase dna; recombinant cloning; dna sequence; isolated dna; significant amounts; acid sequence; amino acid; host cells; sequence encoding; base sequence; cloning vehicle; dna encoding; host cell; sufficiently complementary; cdna encoding; significant amount; mentha piperita; corresponding amino; enhanced expression; aforementioned recombinant; /435/999/

Citation Formats

Croteau, Rodney Bruce, Wildung, Mark Raymond, and Crock, John E. Isolation and bacterial expression of a sesquiterpene synthase CDNA clone from peppermint(mentha .chi. piperita, L.) that produces the aphid alarm pheromone (E)-.beta.-farnesene. United States: N. p., 1999. Web.
Croteau, Rodney Bruce, Wildung, Mark Raymond, & Crock, John E. Isolation and bacterial expression of a sesquiterpene synthase CDNA clone from peppermint(mentha .chi. piperita, L.) that produces the aphid alarm pheromone (E)-.beta.-farnesene. United States.
Croteau, Rodney Bruce, Wildung, Mark Raymond, and Crock, John E. Fri . "Isolation and bacterial expression of a sesquiterpene synthase CDNA clone from peppermint(mentha .chi. piperita, L.) that produces the aphid alarm pheromone (E)-.beta.-farnesene". United States. https://www.osti.gov/servlets/purl/872786.
@article{osti_872786,
title = {Isolation and bacterial expression of a sesquiterpene synthase CDNA clone from peppermint(mentha .chi. piperita, L.) that produces the aphid alarm pheromone (E)-.beta.-farnesene},
author = {Croteau, Rodney Bruce and Wildung, Mark Raymond and Crock, John E},
abstractNote = {A cDNA encoding (E)-.beta.-farnesene synthase from peppermint (Mentha piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of (E)-.beta.-farnesene synthase (SEQ ID NO:2), from peppermint (Mentha piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for (E)-.beta.-farnesene synthase, or for a base sequence sufficiently complementary to at least a portion of (E)-.beta.-farnesene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (E)-.beta.-farnesene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant (E)-.beta.-farnesene synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant (E)-.beta.-farnesene synthase may be used to obtain expression or enhanced expression of (E)-.beta.-farnesene synthase in plants in order to enhance the production of (E)-.beta.-farnesene, or may be otherwise employed for the regulation or expression of (E)-.beta.-farnesene synthase, or the production of its product.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}

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Works referenced in this record:

Purification and Characterization of Trans-β-farnesene Synthase from Maritime Pine (Pinus pinaster Ait.) Needles
journal, June 1995


Isolation of secretory cells from plant glandular trichomes and their use in biosynthetic studies of monoterpenes and other gland products
journal, January 1992


Improved method for the isolation of RNA from plant tissues
journal, May 1987