Title: Isolation and bacterial expression of a sesquiterpene synthase CDNA clone from peppermint(mentha .chi. piperita, L.) that produces the aphid alarm pheromone (E)-.beta.-farnesene

Abstract

A cDNA encoding (E)-.beta.-farnesene synthase from peppermint (Mentha piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of (E)-.beta.-farnesene synthase (SEQ ID NO:2), from peppermint (Mentha piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for (E)-.beta.-farnesene synthase, or for a base sequence sufficiently complementary to at least a portion of (E)-.beta.-farnesene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (E)-.beta.-farnesene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant (E)-.beta.-farnesene synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant (E)-.beta.-farnesene synthase may be used to obtain expression or enhanced expression of (E)-.beta.-farnesene synthase in plants in order to enhance the production of (E)-.beta.-farnesene, or may be otherwise employed for the regulation or expression of (E)-.beta.-farnesene synthase, or the production of its product.

Inventors:

Croteau, Rodney Bruce ^[1]; Wildung, Mark Raymond ^[2]; Crock, John E ^[3]

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+ Show Inventor Affiliations

1. Pullman, WA
2. Colfax, WA
3. Moscow, ID

Issue Date:

1999-01-01

Research Org.:

Washington State University Research Foundation (Pullman, WA)

OSTI Identifier:

872786

Patent Number(s):

6008043

Assignee:

Washington State University Research Foundation (Pullman, WA)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N9/88 - Lyases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P5/007 - {containing one or more isoprene units, i.e. terpenes

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DOE Contract Number:  

FG03-96ER20212

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

isolation; bacterial; expression; sesquiterpene; synthase; cdna; clone; peppermint; mentha; chi; piperita; produces; aphid; alarm; pheromone; beta; -farnesene; encoding; isolated; sequenced; corresponding; amino; acid; sequence; determined; accordingly; dna; seq; provided; codes; aspects; replicable; recombinant; cloning; vehicles; code; base; sufficiently; complementary; portion; rna; enable; hybridization; therewith; modified; host; cells; transformed; transfected; infected; injected; vehicle; systems; methods; aforementioned; facilitate; production; purification; significant; amounts; obtain; enhanced; plants; enhance; otherwise; employed; regulation; product; sequence sufficiently; modified host; cloning vehicles; cdna clone; recombinant expression; replicable recombinant; obtain expression; otherwise employed; enable hybridization; hybridization therewith; synthase dna; recombinant cloning; dna sequence; isolated dna; significant amounts; acid sequence; amino acid; host cells; sequence encoding; base sequence; cloning vehicle; dna encoding; host cell; sufficiently complementary; cdna encoding; significant amount; mentha piperita; corresponding amino; enhanced expression; aforementioned recombinant; /435/999/