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Title: Isolation and bacterial expression of a sesquiterpene synthase CDNA clone from peppermint(mentha .chi. piperita, L.) that produces the aphid alarm pheromone (E)-.beta.-farnesene

Abstract

A cDNA encoding (E)-.beta.-farnesene synthase from peppermint (Mentha piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of (E)-.beta.-farnesene synthase (SEQ ID NO:2), from peppermint (Mentha piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for (E)-.beta.-farnesene synthase, or for a base sequence sufficiently complementary to at least a portion of (E)-.beta.-farnesene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (E)-.beta.-farnesene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant (E)-.beta.-farnesene synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant (E)-.beta.-farnesene synthase may be used to obtain expression or enhanced expression of (E)-.beta.-farnesene synthase in plants in order to enhance the production of (E)-.beta.-farnesene, or may be otherwise employed for the regulation or expression of (E)-.beta.-farnesene synthase, or the production of its product.

Inventors:
 [1];  [2];  [3]
  1. (Pullman, WA)
  2. (Colfax, WA)
  3. (Moscow, ID)
Issue Date:
Research Org.:
Washington State University Research Foundation (Pullman, WA)
OSTI Identifier:
872786
Patent Number(s):
6008043
Assignee:
Washington State University Research Foundation (Pullman, WA) OAK
DOE Contract Number:  
FG03-96ER20212
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
isolation; bacterial; expression; sesquiterpene; synthase; cdna; clone; peppermint; mentha; chi; piperita; produces; aphid; alarm; pheromone; beta; -farnesene; encoding; isolated; sequenced; corresponding; amino; acid; sequence; determined; accordingly; dna; seq; provided; codes; aspects; replicable; recombinant; cloning; vehicles; code; base; sufficiently; complementary; portion; rna; enable; hybridization; therewith; modified; host; cells; transformed; transfected; infected; injected; vehicle; systems; methods; aforementioned; facilitate; production; purification; significant; amounts; obtain; enhanced; plants; enhance; otherwise; employed; regulation; product; sequence sufficiently; modified host; cloning vehicles; cdna clone; recombinant expression; replicable recombinant; obtain expression; otherwise employed; enable hybridization; hybridization therewith; synthase dna; recombinant cloning; dna sequence; isolated dna; significant amounts; acid sequence; amino acid; host cells; sequence encoding; base sequence; cloning vehicle; dna encoding; host cell; sufficiently complementary; cdna encoding; significant amount; mentha piperita; corresponding amino; enhanced expression; aforementioned recombinant; /435/999/

Citation Formats

Croteau, Rodney Bruce, Wildung, Mark Raymond, and Crock, John E. Isolation and bacterial expression of a sesquiterpene synthase CDNA clone from peppermint(mentha .chi. piperita, L.) that produces the aphid alarm pheromone (E)-.beta.-farnesene. United States: N. p., 1999. Web.
Croteau, Rodney Bruce, Wildung, Mark Raymond, & Crock, John E. Isolation and bacterial expression of a sesquiterpene synthase CDNA clone from peppermint(mentha .chi. piperita, L.) that produces the aphid alarm pheromone (E)-.beta.-farnesene. United States.
Croteau, Rodney Bruce, Wildung, Mark Raymond, and Crock, John E. Fri . "Isolation and bacterial expression of a sesquiterpene synthase CDNA clone from peppermint(mentha .chi. piperita, L.) that produces the aphid alarm pheromone (E)-.beta.-farnesene". United States. https://www.osti.gov/servlets/purl/872786.
@article{osti_872786,
title = {Isolation and bacterial expression of a sesquiterpene synthase CDNA clone from peppermint(mentha .chi. piperita, L.) that produces the aphid alarm pheromone (E)-.beta.-farnesene},
author = {Croteau, Rodney Bruce and Wildung, Mark Raymond and Crock, John E.},
abstractNote = {A cDNA encoding (E)-.beta.-farnesene synthase from peppermint (Mentha piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of (E)-.beta.-farnesene synthase (SEQ ID NO:2), from peppermint (Mentha piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for (E)-.beta.-farnesene synthase, or for a base sequence sufficiently complementary to at least a portion of (E)-.beta.-farnesene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (E)-.beta.-farnesene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant (E)-.beta.-farnesene synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant (E)-.beta.-farnesene synthase may be used to obtain expression or enhanced expression of (E)-.beta.-farnesene synthase in plants in order to enhance the production of (E)-.beta.-farnesene, or may be otherwise employed for the regulation or expression of (E)-.beta.-farnesene synthase, or the production of its product.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}

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