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Title: Detection of nucleic acid sequences by invader-directed cleavage

Abstract

The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The 5' nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based by charge.

Inventors:
 [1];  [1];  [1];  [1];  [1]
  1. (Madison, WI)
Issue Date:
Research Org.:
Third Wave Technologies, Inc., Madison, WI (United States)
OSTI Identifier:
872754
Patent Number(s):
6001567
Assignee:
Third Wave Technologies, Inc. (CA) CHO
DOE Contract Number:  
FG02-94ER81891
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
detection; nucleic; acid; sequences; invader-directed; cleavage; relates; means; characterization; variations; methods; forming; structure; target; sequence; cleaving; site-specific; manner; nuclease; activity; variety; enzymes; cleave; target-dependent; indicating; presence; specific; devices; separation; molecules; based; charge; acid molecule; cleavage structure; acid molecules; acid sequence; nucleic acid; acid sequences; target sequence; acid cleavage; specific nucleic; molecules based; specific variations; site-specific manner; nuclease activity; target-dependent cleavage; specific manner; /435/999/

Citation Formats

Brow, Mary Ann D., Hall, Jeff Steven Grotelueschen, Lyamichev, Victor, Olive, David Michael, and Prudent, James Robert. Detection of nucleic acid sequences by invader-directed cleavage. United States: N. p., 1999. Web.
Brow, Mary Ann D., Hall, Jeff Steven Grotelueschen, Lyamichev, Victor, Olive, David Michael, & Prudent, James Robert. Detection of nucleic acid sequences by invader-directed cleavage. United States.
Brow, Mary Ann D., Hall, Jeff Steven Grotelueschen, Lyamichev, Victor, Olive, David Michael, and Prudent, James Robert. Fri . "Detection of nucleic acid sequences by invader-directed cleavage". United States. https://www.osti.gov/servlets/purl/872754.
@article{osti_872754,
title = {Detection of nucleic acid sequences by invader-directed cleavage},
author = {Brow, Mary Ann D. and Hall, Jeff Steven Grotelueschen and Lyamichev, Victor and Olive, David Michael and Prudent, James Robert},
abstractNote = {The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The 5' nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based by charge.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}

Patent:

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