Detection of nucleic acids by multiple sequential invasive cleavages
Abstract
The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.
- Inventors:
-
- Madison, WI
- Issue Date:
- Research Org.:
- Third Wave Technologies, Inc., Madison, WI (United States)
- OSTI Identifier:
- 872708
- Patent Number(s):
- 5994069
- Assignee:
- Third Wave Technologies, Inc. (Madison, WI)
- DOE Contract Number:
- FG02-94ER81891
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- detection; nucleic; acids; multiple; sequential; invasive; cleavages; relates; means; characterization; acid; sequences; variations; methods; forming; cleavage; structure; target; sequence; cleaving; site-specific; manner; structure-specific; nuclease; activity; variety; enzymes; cleave; target-dependent; indicating; presence; specific; devices; separation; molecules; based; charge; provides; non-target; products; via; formation; complete; activated; protein; binding; region; sensitive; human; cytomegalovirus; sample; acid molecule; provides methods; cleavage structure; acid molecules; acid sequence; nucleic acid; acid sequences; nucleic acids; target sequence; acid cleavage; specific nucleic; structure-specific nuclease; molecules based; specific variations; site-specific manner; nuclease activity; target-dependent cleavage; specific manner; /435/
Citation Formats
Hall, Jeff G, Lyamichev, Victor I, Mast, Andrea L, and Brow, Mary Ann D. Detection of nucleic acids by multiple sequential invasive cleavages. United States: N. p., 1999.
Web.
Hall, Jeff G, Lyamichev, Victor I, Mast, Andrea L, & Brow, Mary Ann D. Detection of nucleic acids by multiple sequential invasive cleavages. United States.
Hall, Jeff G, Lyamichev, Victor I, Mast, Andrea L, and Brow, Mary Ann D. Fri .
"Detection of nucleic acids by multiple sequential invasive cleavages". United States. https://www.osti.gov/servlets/purl/872708.
@article{osti_872708,
title = {Detection of nucleic acids by multiple sequential invasive cleavages},
author = {Hall, Jeff G and Lyamichev, Victor I and Mast, Andrea L and Brow, Mary Ann D},
abstractNote = {The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}