DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Detection of nucleic acids by multiple sequential invasive cleavages

Abstract

The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.

Inventors:
 [1];  [1];  [1];  [1]
  1. Madison, WI
Issue Date:
Research Org.:
Third Wave Technologies, Inc., Madison, WI (United States)
OSTI Identifier:
872708
Patent Number(s):
5994069
Assignee:
Third Wave Technologies, Inc. (Madison, WI)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG02-94ER81891
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
detection; nucleic; acids; multiple; sequential; invasive; cleavages; relates; means; characterization; acid; sequences; variations; methods; forming; cleavage; structure; target; sequence; cleaving; site-specific; manner; structure-specific; nuclease; activity; variety; enzymes; cleave; target-dependent; indicating; presence; specific; devices; separation; molecules; based; charge; provides; non-target; products; via; formation; complete; activated; protein; binding; region; sensitive; human; cytomegalovirus; sample; acid molecule; provides methods; cleavage structure; acid molecules; acid sequence; nucleic acid; acid sequences; nucleic acids; target sequence; acid cleavage; specific nucleic; structure-specific nuclease; molecules based; specific variations; site-specific manner; nuclease activity; target-dependent cleavage; specific manner; /435/

Citation Formats

Hall, Jeff G, Lyamichev, Victor I, Mast, Andrea L, and Brow, Mary Ann D. Detection of nucleic acids by multiple sequential invasive cleavages. United States: N. p., 1999. Web.
Hall, Jeff G, Lyamichev, Victor I, Mast, Andrea L, & Brow, Mary Ann D. Detection of nucleic acids by multiple sequential invasive cleavages. United States.
Hall, Jeff G, Lyamichev, Victor I, Mast, Andrea L, and Brow, Mary Ann D. Fri . "Detection of nucleic acids by multiple sequential invasive cleavages". United States. https://www.osti.gov/servlets/purl/872708.
@article{osti_872708,
title = {Detection of nucleic acids by multiple sequential invasive cleavages},
author = {Hall, Jeff G and Lyamichev, Victor I and Mast, Andrea L and Brow, Mary Ann D},
abstractNote = {The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}

Works referenced in this record:

Recent advances in the polymerase chain reaction
journal, June 1991


Detection of human cytomegalovirus in peripheral mononuclear cells and urine samples using PCR
journal, February 1992


RNA Molecules That Specifically and Stoichiometrically Bind Aminoglycoside Antibiotics with High Affinities
journal, January 1996


Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
journal, August 1970


Early diagnosis and successful treatment of acute cytomegalovirus encephalitis in a renal transplant recipient
journal, January 1992


Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase
journal, August 1988


Identification of Hepatitis C Virus Genotypes among Hospitalized Patients in British Columbia, Canada
journal, April 1995


Characterization of the 5′ to 3′ exonuclease associated with Thermus aquaticus DNA polymerase
journal, January 1990


Isolation, Characterization, and Expression in Escherichia coli of the DNA Polymerase Gene from Thermus aquaticus
journal, April 1989


DNA sequencing: modular primers assembled from a library of hexamers or pentamers.
journal, May 1993


Molecular cloning and nucleotide sequence of the DNA polymerase gene from Thermus flavus
journal, January 1992


Strand Separation and Specific Recombination in Deoxyribonucleic Acids: Physical Chemical Studies
journal, April 1960


Deoxyribonucleic Acid Polymerase: Two Distinct Enzymes in One Polypeptide
journal, January 1972


Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
journal, May 1986


Hepatitis C: progress and problems.
journal, October 1994


Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase.
journal, August 1991


On the fidelity of mRNA translation in the nuclease-treated rabbit reticulocyte lysate system
journal, January 1989


Monitoring of patients for cytomegalovirus after organ transplantation by centrifugation culture and PCR
journal, December 1992


An active fragment of DNA polymerase produced by proteolytic cleavage
journal, December 1969


Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication.
journal, March 1990


Instability and decay of the primary structure of DNA
journal, April 1993


Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
journal, January 1988


Replacement of potassium chloride by potassium glutamate dramatically enhances protein-DNA interactions in vitro
journal, April 1987


On the Regulation of Gene Activity
journal, January 1961


Genetic disease detection and DNA amplification using cloned thermostable ligase.
journal, January 1991


Major Groove Recognition Elements in the Middle of the T7 RNA Polymerase Promoter
journal, January 1996


Structure-specific endonucleolytic cleavage of nucleic acids by eubacterial DNA polymerases
journal, May 1993


Intervening sequences in an Archaea DNA polymerase gene.
journal, June 1992


Compilation and alignment of DNA polymerase sequences
journal, January 1991


Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements
journal, June 1983


Effects of primer-template mismatches on the polymerase chain reaction: Human immunodeficiency virus type 1 model studies
journal, January 1990


Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format.
journal, February 1989


Crystal structure of Thermus aquaticus DNA polymerase
journal, August 1995


Molecular mechanics of nucleic acid sequence amplification
journal, June 1993


A helical arch allowing single-stranded DNA to thread through T5 5'-exonuclease
journal, July 1996


Detection of human cytomegalovirus in plasma of AIDS patients during acute visceral disease by DNA amplification.
journal, January 1992


Heat-induced depyrimidination of deoxyribonucleic acid in neutral solution
journal, December 1973


Rapid Detection of Cytomegalovirus dna and rna in Blood of Renal Transplant Patients by in Vitro Enzymatic Amplification
journal, January 1991


Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase
journal, August 1991


Polymerase chain reaction in detection of CMV DNA in renal allograft recipients
journal, June 1992


The ligase chain reaction in a PCR world.
journal, August 1991


The DNA polymerase gene from the hyperthermophilic marine archaebacterium, pyrococcus furiosus , shows sequence homology with α-like DNA polymerases
journal, January 1991


RNA Libraries and RNA Recognitiona
journal, May 1996


A small catalytic oligoribonucleotide
journal, August 1987


Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli
journal, December 1990


A novel transcription property of SP6 and T7 RNA polymerases: dependence on template structure
journal, January 1985


A thermodynamic study of unusually stable RNA and DNA hairpins
journal, January 1991


Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates
journal, January 1987


Strand Separation and Specific Recombination in Deoxyribonucleic Acids: Biological Studies
journal, April 1960