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Title: Nucleic acid indexing

Abstract

A restriction site indexing method for selectively amplifying any fragment generated by a Class II restriction enzyme includes adaptors specific to fragment ends containing adaptor indexing sequences complementary to fragment indexing sequences near the termini of fragments generated by Class II enzyme cleavage. A method for combinatorial indexing facilitates amplification of restriction fragments whose sequence is not known.

Inventors:
 [1];  [2]
  1. Madison, WI
  2. Bellevue, WA
Issue Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States)
OSTI Identifier:
872707
Patent Number(s):
5994068
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG02-91ER61122
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
nucleic; acid; indexing; restriction; site; method; selectively; amplifying; fragment; generated; ii; enzyme; adaptors; specific; containing; adaptor; sequences; complementary; near; termini; fragments; cleavage; combinatorial; facilitates; amplification; sequence; nucleic acid; restriction enzyme; restriction fragments; restriction site; indexing sequences; selectively amplifying; sequences complementary; site indexing; fragments generated; fragment generated; fragment indexing; indexing method; ii restriction; restriction fragment; acid indexing; adaptors specific; adaptor indexing; containing adaptor; /435/999/

Citation Formats

Guilfoyle, Richard A, and Guo, Zhen. Nucleic acid indexing. United States: N. p., 1999. Web.
Guilfoyle, Richard A, & Guo, Zhen. Nucleic acid indexing. United States.
Guilfoyle, Richard A, and Guo, Zhen. Fri . "Nucleic acid indexing". United States. https://www.osti.gov/servlets/purl/872707.
@article{osti_872707,
title = {Nucleic acid indexing},
author = {Guilfoyle, Richard A and Guo, Zhen},
abstractNote = {A restriction site indexing method for selectively amplifying any fragment generated by a Class II restriction enzyme includes adaptors specific to fragment ends containing adaptor indexing sequences complementary to fragment indexing sequences near the termini of fragments generated by Class II enzyme cleavage. A method for combinatorial indexing facilitates amplification of restriction fragments whose sequence is not known.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}

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Works referenced in this record:

Branch capture reactions: effect of recipient structure
journal, January 1990


Covalently linked sequencing primer (splinkers) for sequence analysis of restriction fragments
journal, January 1986


"RFLP subtraction": a method for making libraries of polymorphic markers.
journal, June 1994


Branch capture reactions: displacers derived from asymmetric PCR
journal, January 1991


Ligation-mediated PCR of restriction fragments from large DNA molecules.
journal, August 1992


Cloning the differences between two complex genomes
journal, February 1993


AFLP: a new technique for DNA fingerprinting
journal, January 1995


Branch migration mediated DNA labeling and cloning
journal, October 1989


Ligation-mediated PCR amplification of specific fragments from a Class-II restriction endonuclease total digest
journal, May 1997