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Title: Inhibition of selectin binding

Abstract

This invention provides compositions for inhibiting the binding between two cells, one expressing P- or L-selectin on the surface and the other expressing the corresponding ligand. A covalently crosslinked lipid composition is prepared having saccharides and acidic group on separate lipids. The composition is then interposed between the cells so as to inhibit binding. Inhibition can be achieved at an effective oligosaccharide concentration as low as 10.sup.6 fold below that of the free saccharide. Since selectins are involved in recruiting cells to sites of injury, these composition scan be used to palliate certain inflammatory and immunological conditions.

Inventors:
 [1];  [2];  [3];  [2]
  1. Rodeo, CA
  2. Albany, CA
  3. New Delhi, IN
Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
OSTI Identifier:
872672
Patent Number(s):
5985852
Assignee:
Regents of University of California ()
Patent Classifications (CPCs):
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
inhibition; selectin; binding; provides; compositions; inhibiting; cells; expressing; p-; l-selectin; surface; corresponding; ligand; covalently; crosslinked; lipid; composition; prepared; saccharides; acidic; separate; lipids; interposed; inhibit; achieved; effective; oligosaccharide; concentration; 10; fold; below; free; saccharide; selectins; involved; recruiting; sites; injury; scan; palliate; inflammatory; immunological; conditions; recruiting cells; provides compositions; selectin binding; separate lipids; oligosaccharide concentration; expressing p-; fold below; free saccharide; lipid composition; effective oligosaccharide; covalently crosslinked; crosslinked lipid; corresponding ligand; immunological conditions; inhibit binding; /514/424/999/

Citation Formats

Nagy, Jon O, Spevak, Wayne R, Dasgupta, Falguni, and Bertozzi, Caroline. Inhibition of selectin binding. United States: N. p., 1999. Web.
Nagy, Jon O, Spevak, Wayne R, Dasgupta, Falguni, & Bertozzi, Caroline. Inhibition of selectin binding. United States.
Nagy, Jon O, Spevak, Wayne R, Dasgupta, Falguni, and Bertozzi, Caroline. Fri . "Inhibition of selectin binding". United States. https://www.osti.gov/servlets/purl/872672.
@article{osti_872672,
title = {Inhibition of selectin binding},
author = {Nagy, Jon O and Spevak, Wayne R and Dasgupta, Falguni and Bertozzi, Caroline},
abstractNote = {This invention provides compositions for inhibiting the binding between two cells, one expressing P- or L-selectin on the surface and the other expressing the corresponding ligand. A covalently crosslinked lipid composition is prepared having saccharides and acidic group on separate lipids. The composition is then interposed between the cells so as to inhibit binding. Inhibition can be achieved at an effective oligosaccharide concentration as low as 10.sup.6 fold below that of the free saccharide. Since selectins are involved in recruiting cells to sites of injury, these composition scan be used to palliate certain inflammatory and immunological conditions.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}

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Works referenced in this record:

Molecular Architecture and Function of Polymeric Oriented Systems: Models for the Study of Organization, Surface Recognition, and Dynamics of Biomembranes
journal, January 1988


Glycolipids as Immunomodulators: Syntheses and Properties
journal, December 1991


Peptides Which Bind to E-selectin and Block Neutrophil Adhesion
journal, September 1995


Design and Synthesis of Glycomimetic Prototypes - A Model Sialyl Lewis x Ligand for E-Selectin
journal, January 1994


Ligand Recognition by E-Selectin: Synthesis, Inhibitory Activity and Conformational Analysis of Bivalent Sialyl Lewis x Analogs
journal, January 1995


ELAM-1 mediates cell adhesion by recognition of a carbohydrate ligand, sialyl-Lex
journal, November 1990


Intervention of carbohydrate recognition by proteins and nucleic acids.
journal, October 1996


Lymphocyte homing and leukocyte rolling and migration are impaired in L-selectin-deficient mice
journal, July 1994


Synthesis of phospholipid-inhibitor conjugates by enzymic transphosphatidylation with phospholipase D
journal, November 1993


Supramolecular assemblies of diacetylenic aldonamides
journal, September 1991


Synthesis of antigenic carbohydrate polymers recognized by lectins and antibodies
journal, January 1988


P-selectin glycoprotein ligand-1 mediates rolling of human neutrophils on P-selectin.
journal, February 1995


Granule membrane protein 140 (GMP140) binds to carcinomas and carcinoma-derived cell lines.
journal, March 1992


Selectin-Carbohydrate Interactions and the Initiation of the Inflammatory Response
journal, June 1995


Liposome-like fucopeptides as sialyl lewis X mimetics
journal, November 1996


Enzyme-catalyzed synthesis of carbohydrates
journal, January 1989


A ‘litmus test’ for molecular recognition using artificial membranes
journal, February 1996


Inositol polyanions. Noncarbohydrate inhibitors of L- and P-selectin that block inflammation.
journal, May 1994


Higher-affinity oligosaccharide ligands for E-selectin.
journal, March 1993


Tumor necrosis factor participates in the pathogenesis of acute immune complex alveolitis in the rat.
journal, December 1989


Molecular assemblies of functionalized polydiacetylenes
journal, January 1995


Sialyl Lewis x Liposomes as a Multivalent Ligand and Inhibitor of E-Selectin Mediated Cellular Adhesion
journal, January 1996


The chemical-enzymatic synthesis of a carbon glycoside of N-acetyl neuraminic acid
journal, August 1991


Polymerizable Phospholipid Analogues—New Stable Biomembrane and Cell Models
journal, November 1980


Shear-dependent inhibition of granulocyte adhesion to cultured endothelium by dextran sulfate
journal, April 1989


Polymerized liposomes containing C-glycosides of sialic acid: potent inhibitors of influenza virus in vitro infectivity
journal, February 1993


Synthesis of bioactive sialosides
journal, January 1993


Liposomes from polymerizable phospholipids
journal, November 1983


Leukocyte accumulation promoting fibrin deposition is mediated in vivo by P-selectin on adherent platelets
journal, October 1992


Tyrosine Sulfation of P-selectin Glycoprotein Ligand-1 Is Required for High Affinity Binding to P-selectin
journal, September 1995


Polydiacetylene Liposomes Functionalized with Sialic Acid Bind and Colorimetrically Detect Influenza Virus
journal, January 1995


The complement binding-like domains of the murine homing receptor facilitate lectin activity.
journal, October 1991


Myeloperoxidase activity as a quantitative assessment of neutrophil infiltration into ischemie myocardium
journal, November 1985


Leukocyte trafficking mediated by selectin-carbohydrate interactions
journal, May 1995


Supramolecular assemblies, a crystal structure, and a polymer of N-diacetylenic gluconamides
journal, September 1991


An Efficient Synthesis of N -Allylglycosylamides from Unprotected Carbohydrates
journal, January 1996


Sulphation requirement for GlyCAM-1, an endothelial ligand for L-selectin
journal, February 1993


A sulfated peptide segment at the amino terminus of PSGL-1 is critical for P-selectin binding
journal, October 1995


Synthetic Bilayer Membranes with Anionic Head Groups
journal, June 1978


Glycomimetic selectin inhibitors: (α-D-mannopyranosyloxy)methylbiphenyls
journal, March 1996


Rapid neutrophil adhesion to activated endothelium mediated by GMP-140
journal, February 1990


Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system.
journal, December 1987


Possible role for cell-surface carbohydrate-binding molecules in lymphocyte recirculation.
journal, March 1983


Rational Design and Synthesis of a 1,1-Linked Disaccharide That Is 5 Times as Active as Sialyl Lewis X in Binding to E-Selectin
journal, January 1996


CD62/P-selectin recognition of myeloid and tumor cell sulfatides
journal, October 1991


Sulfated disaccharide with greater inhibitory potency for L-selectin than sialyl Lewis x
journal, November 1995


Calcium-dependent heparin-like ligands for L-selectin in nonlymphoid endothelial cells
journal, July 1993


Highly stereoselective c-allylation of glycopyranosides with allylsilanes catalyzed by silyl triflate or iodosilane
journal, January 1984


The HNK-1 reactive sulfoglucuronyl glycolipids are ligands for L-selectin and P-selectin but not E-selectin.
journal, February 1993


GMP-140 binds to a glycoprotein receptor on human neutrophils: evidence for a lectin-like interaction.
journal, February 1991


Lifetime of the P-selectin-carbohydrate bond and its response to tensile force in hydrodynamic flow
journal, April 1995


Enzyme-catalyzed oligosaccharide synthesis
journal, May 1992