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Title: Methods for immobilizing nucleic acids on a gel substrate

Abstract

A method for labeling oligonucleotide molecules, and for immobilizing oligonucleotide and DNA molecules is provided comprising modifying the molecules to create a chemically active group, and contacting activated fluorescent dyes to the region. A method for preparing an immobilization substrate is also provided comprising modifying a gel to contain desired functional groups which covalently interact with certain moieties of the oligonucleotide molecules. A method for immobilizing biomolecules and other molecules within a gel by copolymerization of allyl-substituted oligonucleotides, DNA and proteins with acrylamide is also provided.

Inventors:
 [1];  [2];  [1];  [1];  [1];  [1]
  1. Moscow, RU
  2. Samara, RU
Issue Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
OSTI Identifier:
872654
Patent Number(s):
5981734
Assignee:
University of Chicago (Chicago, IL)
Patent Classifications (CPCs):
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS
DOE Contract Number:  
W-31109-ENG-38
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
methods; immobilizing; nucleic; acids; substrate; method; labeling; oligonucleotide; molecules; dna; provided; comprising; modifying; create; chemically; active; contacting; activated; fluorescent; dyes; region; preparing; immobilization; contain; desired; functional; covalently; interact; moieties; biomolecules; copolymerization; allyl-substituted; oligonucleotides; proteins; acrylamide; nucleic acid; provided comprising; nucleic acids; oligonucleotide molecules; fluorescent dyes; dna molecules; dna molecule; fluorescent dye; chemically active; immobilizing biomolecules; /999/428/

Citation Formats

Mirzabekov, Andrei Darievich, Proudnikov, Dimitri Y, Timofeev, Edward N, Kochetkova, Svetlana V, Florentiev, Vladimir L, and Shick, Valentine V. Methods for immobilizing nucleic acids on a gel substrate. United States: N. p., 1999. Web.
Mirzabekov, Andrei Darievich, Proudnikov, Dimitri Y, Timofeev, Edward N, Kochetkova, Svetlana V, Florentiev, Vladimir L, & Shick, Valentine V. Methods for immobilizing nucleic acids on a gel substrate. United States.
Mirzabekov, Andrei Darievich, Proudnikov, Dimitri Y, Timofeev, Edward N, Kochetkova, Svetlana V, Florentiev, Vladimir L, and Shick, Valentine V. Fri . "Methods for immobilizing nucleic acids on a gel substrate". United States. https://www.osti.gov/servlets/purl/872654.
@article{osti_872654,
title = {Methods for immobilizing nucleic acids on a gel substrate},
author = {Mirzabekov, Andrei Darievich and Proudnikov, Dimitri Y and Timofeev, Edward N and Kochetkova, Svetlana V and Florentiev, Vladimir L and Shick, Valentine V},
abstractNote = {A method for labeling oligonucleotide molecules, and for immobilizing oligonucleotide and DNA molecules is provided comprising modifying the molecules to create a chemically active group, and contacting activated fluorescent dyes to the region. A method for preparing an immobilization substrate is also provided comprising modifying a gel to contain desired functional groups which covalently interact with certain moieties of the oligonucleotide molecules. A method for immobilizing biomolecules and other molecules within a gel by copolymerization of allyl-substituted oligonucleotides, DNA and proteins with acrylamide is also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}

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Works referenced in this record:

Regioselective immobilization of short oligonucleotides to acrylic copolymer gels
journal, August 1996


Chemical Methods of DNA and RNA Fluorescent Labeling
journal, November 1996