Metal resistance sequences and transgenic plants

Meagher, Richard Brian; Summers, Anne O; Rugh, Clayton L

Title: Metal resistance sequences and transgenic plants

Abstract

The present invention provides nucleic acid sequences encoding a metal ion resistance protein, which are expressible in plant cells. The metal resistance protein provides for the enzymatic reduction of metal ions including but not limited to divalent Cu, divalent mercury, trivalent gold, divalent cadmium, lead ions and monovalent silver ions. Transgenic plants which express these coding sequences exhibit increased resistance to metal ions in the environment as compared with plants which have not been so genetically modified. Transgenic plants with improved resistance to organometals including alkylmercury compounds, among others, are provided by the further inclusion of plant-expressible organometal lyase coding sequences, as specifically exemplified by the plant-expressible merB coding sequence. Furthermore, these transgenic plants which have been genetically modified to express the metal resistance coding sequences of the present invention can participate in the bioremediation of metal contamination via the enzymatic reduction of metal ions. Transgenic plants resistant to organometals can further mediate remediation of organic metal compounds, for example, alkylmetal compounds including but not limited to methyl mercury, methyl lead compounds, methyl cadmium and methyl arsenic compounds, in the environment by causing the freeing of mercuric or other metal ions and the reduction of the ionic mercury or othermore » « less

Inventors:

Meagher, Richard Brian ^[1]; Summers, Anne O ^[1]; Rugh, Clayton L ^[1]

Athens, GA

Issue Date:: 1999-10-12

OSTI Identifier:: 872584

Patent Number(s):: 5965796

Application Number:: 08/878,957

Assignee:: University of Georgia Research Foundation Inc. (Athens, GA)

Patent Classifications (CPCs):: B - PERFORMING OPERATIONS B09 - DISPOSAL OF SOLID WASTE B09C - RECLAMATION OF CONTAMINATED SOIL

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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B - PERFORMING OPERATIONS B09 - DISPOSAL OF SOLID WASTE B09C - RECLAMATION OF CONTAMINATED SOIL
B09C1/105 - {using fungi or plants}

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/52 - Genes encoding for enzymes or proenzymes
C12N15/8259 - {Phytoremediation}
C12N15/8271 - {for stress resistance, e.g. heavy metal resistance}
C12N15/8274 - {for herbicide resistance}
C12N9/0091 - {oxidizing metal ions
C12N9/88 - Lyases

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Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: metal; resistance; sequences; transgenic; plants; provides; nucleic; acid; encoding; protein; expressible; plant; cells; enzymatic; reduction; including; limited; divalent; cu; mercury; trivalent; gold; cadmium; lead; monovalent; silver; express; coding; exhibit; increased; environment; compared; genetically; modified; improved; organometals; alkylmercury; compounds; provided; inclusion; plant-expressible; organometal; lyase; specifically; exemplified; merb; sequence; furthermore; participate; bioremediation; contamination; via; resistant; mediate; remediation; organic; example; alkylmetal; methyl; arsenic; causing; freeing; mercuric; ionic; toxic; elemental; metals; sequences encoding; compounds including; metal contamination; genetically modified; mercury compounds; coding sequences; metal compound; metal compounds; acid sequence; nucleic acid; acid sequences; transgenic plants; plant cell; plant cells; improved resistance; elemental mercury; metal resistance; specifically exemplified; arsenic compounds; coding sequence; exhibit increased; resistance protein; organic metal; metals including; transgenic plant; increased resistance; /800/435/999/

Citation Formats


                    Meagher, Richard Brian, Summers, Anne O, and Rugh, Clayton L. Metal resistance sequences and transgenic plants.  United States: N. p., 1999. 
        Web.

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                    Meagher, Richard Brian, Summers, Anne O, & Rugh, Clayton L. Metal resistance sequences and transgenic plants.  United States.

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                    Meagher, Richard Brian, Summers, Anne O, and Rugh, Clayton L. Tue .  
        "Metal resistance sequences and transgenic plants".  United States.  https://www.osti.gov/servlets/purl/872584.

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@article{osti_872584,

  title        = {Metal resistance sequences and transgenic plants},

  author       = {Meagher, Richard Brian and Summers, Anne O and Rugh, Clayton L},

  abstractNote = {The present invention provides nucleic acid sequences encoding a metal ion resistance protein, which are expressible in plant cells. The metal resistance protein provides for the enzymatic reduction of metal ions including but not limited to divalent Cu, divalent mercury, trivalent gold, divalent cadmium, lead ions and monovalent silver ions. Transgenic plants which express these coding sequences exhibit increased resistance to metal ions in the environment as compared with plants which have not been so genetically modified. Transgenic plants with improved resistance to organometals including alkylmercury compounds, among others, are provided by the further inclusion of plant-expressible organometal lyase coding sequences, as specifically exemplified by the plant-expressible merB coding sequence. Furthermore, these transgenic plants which have been genetically modified to express the metal resistance coding sequences of the present invention can participate in the bioremediation of metal contamination via the enzymatic reduction of metal ions. Transgenic plants resistant to organometals can further mediate remediation of organic metal compounds, for example, alkylmetal compounds including but not limited to methyl mercury, methyl lead compounds, methyl cadmium and methyl arsenic compounds, in the environment by causing the freeing of mercuric or other metal ions and the reduction of the ionic mercury or other metal ions to the less toxic elemental mercury or other metals.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1999},

  month        = {10}

}

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Title: Metal resistance sequences and transgenic plants

Abstract

Citation Formats

Expression of bacterial mercuric ion reductase in Saccharomyces cerevisiae. journal, January 1992

Phytochelatins, a class of heavy-metal-binding peptides from plants, are functionally analogous to metallothioneins journal, January 1987

Organization, Expression, and Evolution of Genes for Mercury Resistance journal, October 1986

Asymmetrical distribution of CpG in an ‘average’ mammalian gene journal, January 1982

The distribution and divergence of DNA sequences related to the Tn21 and Tn501 mer operons journal, September 1988

Mercury and Organomercurial Resistances Determined by Plasmids in Pseudomonas journal, January 1977

Structural Analysis of Plant Genes journal, June 1986

Characterization of translational initiation sites in E. coli journal, January 1982

Transcriptional and post-transcriptional processes regulate expression of RNA encoding the small subunit of ribulose-1, 5-biphosphate carboxylase differently in petunia and in soybean journal, January 1990

Codon usage in plant genes journal, January 1989

Deoxyribonucleic acid sequence of a gene from the Pseudomonas transposon TN501 encoding mercuric reductase journal, August 1983

Physical and genetic map of the organomercury resistance (Omr) and inorganic mercury resistance (Hgr) loci of the IncM plasmid R831b journal, December 1984

Mercury biotransformations and their potential for remediation of mercury contamination journal, January 1992

Mechanisms of microbial resistance and detoxification of mercury and organomercury compounds: physiological, biochemical, and genetic analyses. journal, January 1984

Compound surface modelling and machining journal, April 1988

Phytochelatin Synthesis and Glutathione Levels in Response to Heavy Metals in Tomato Cells journal, December 1987

Mercuric reductase structural genes from plasmid R100 and transposon Tn501: functional domains of the enzyme journal, January 1985

Bacterial organomercurial lyase: overproduction, isolation, and characterization journal, November 1986

Mammalian Metallothionein Functions in Plants journal, October 1987

Mechanistic studies of a protonolytic organomercurial cleaving enzyme: bacterial organomercurial lyase journal, November 1986

Plasmid-determined resistance to antimicrobial drugs and toxic metal ions in bacteria. journal, January 1983

Mercuric reductase from R-plasmid NR1: characterization and mechanistic study journal, February 1983

Pressurized Ventilation in the Yellow Waterlily journal, October 1981

Internal Winds in Water Lilies: An Adaptation for Life in Anaerobic Sediments journal, November 1980

Expression of bacterial mercuric ion reductase in Saccharomyces cerevisiae.
journal, January 1992

Phytochelatins, a class of heavy-metal-binding peptides from plants, are functionally analogous to metallothioneins
journal, January 1987

Organization, Expression, and Evolution of Genes for Mercury Resistance
journal, October 1986

Asymmetrical distribution of CpG in an ‘average’ mammalian gene
journal, January 1982

The distribution and divergence of DNA sequences related to the Tn21 and Tn501 mer operons
journal, September 1988

Mercury and Organomercurial Resistances Determined by Plasmids in Pseudomonas
journal, January 1977

Structural Analysis of Plant Genes
journal, June 1986

Characterization of translational initiation sites in E. coli
journal, January 1982

Transcriptional and post-transcriptional processes regulate expression of RNA encoding the small subunit of ribulose-1, 5-biphosphate carboxylase differently in petunia and in soybean
journal, January 1990

Codon usage in plant genes
journal, January 1989

Deoxyribonucleic acid sequence of a gene from the Pseudomonas transposon TN501 encoding mercuric reductase
journal, August 1983

Physical and genetic map of the organomercury resistance (Omr) and inorganic mercury resistance (Hgr) loci of the IncM plasmid R831b
journal, December 1984

Mercury biotransformations and their potential for remediation of mercury contamination
journal, January 1992

Mechanisms of microbial resistance and detoxification of mercury and organomercury compounds: physiological, biochemical, and genetic analyses.
journal, January 1984

Compound surface modelling and machining
journal, April 1988

Phytochelatin Synthesis and Glutathione Levels in Response to Heavy Metals in Tomato Cells
journal, December 1987

Mercuric reductase structural genes from plasmid R100 and transposon Tn501: functional domains of the enzyme
journal, January 1985

Bacterial organomercurial lyase: overproduction, isolation, and characterization
journal, November 1986

Mammalian Metallothionein Functions in Plants
journal, October 1987

Mechanistic studies of a protonolytic organomercurial cleaving enzyme: bacterial organomercurial lyase
journal, November 1986

Plasmid-determined resistance to antimicrobial drugs and toxic metal ions in bacteria.
journal, January 1983

Mercuric reductase from R-plasmid NR1: characterization and mechanistic study
journal, February 1983

Pressurized Ventilation in the Yellow Waterlily
journal, October 1981

Internal Winds in Water Lilies: An Adaptation for Life in Anaerobic Sediments
journal, November 1980