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Title: Genetically enhanced cellulase production in Pseudomonas cellulosa using recombinant DNA technology

Abstract

An enhanced strain of Pseudomonas celllulosa was obtained by introducing a recombinant genetic construct comprising a heterologous cellulase gene operably connected to a promoter into ATCC 55702, mutagenizing the transformants by treatment with MNNG, and selecting a high cellulase producing transformant. The transformant, designated Pseudomonas cellulosa ATCC XXXX, exhibits enhanced levels of cellulase production relative to the untransformed Pseudomonas cellulosa strain #142 ATCC 55702.

Inventors:
 [1]
  1. (Knoxville, TN)
Issue Date:
Research Org.:
LOCKHEED MARTIN ENERGY RES COR
OSTI Identifier:
872543
Patent Number(s):
5958740
Assignee:
Lockheed Martin Energy Research Corp. (Oak Ridge, TN) ORNL
DOE Contract Number:  
AC05-96OR22464
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
genetically; enhanced; cellulase; production; pseudomonas; cellulosa; recombinant; dna; technology; strain; celllulosa; obtained; introducing; genetic; construct; comprising; heterologous; operably; connected; promoter; atcc; 55702; mutagenizing; transformants; treatment; mnng; selecting; producing; transformant; designated; xxxx; exhibits; levels; relative; untransformed; 142; cellulase producing; enhanced cellulase; recombinant dna; operably connected; exhibits enhanced; construct comprising; /435/424/

Citation Formats

Dees, H. Craig. Genetically enhanced cellulase production in Pseudomonas cellulosa using recombinant DNA technology. United States: N. p., 1999. Web.
Dees, H. Craig. Genetically enhanced cellulase production in Pseudomonas cellulosa using recombinant DNA technology. United States.
Dees, H. Craig. Fri . "Genetically enhanced cellulase production in Pseudomonas cellulosa using recombinant DNA technology". United States. https://www.osti.gov/servlets/purl/872543.
@article{osti_872543,
title = {Genetically enhanced cellulase production in Pseudomonas cellulosa using recombinant DNA technology},
author = {Dees, H. Craig},
abstractNote = {An enhanced strain of Pseudomonas celllulosa was obtained by introducing a recombinant genetic construct comprising a heterologous cellulase gene operably connected to a promoter into ATCC 55702, mutagenizing the transformants by treatment with MNNG, and selecting a high cellulase producing transformant. The transformant, designated Pseudomonas cellulosa ATCC XXXX, exhibits enhanced levels of cellulase production relative to the untransformed Pseudomonas cellulosa strain #142 ATCC 55702.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}

Patent:

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