Genetically enhanced cellulase production in Pseudomonas cellulosa using recombinant DNA technology
Abstract
An enhanced strain of Pseudomonas celllulosa was obtained by introducing a recombinant genetic construct comprising a heterologous cellulase gene operably connected to a promoter into ATCC 55702, mutagenizing the transformants by treatment with MNNG, and selecting a high cellulase producing transformant. The transformant, designated Pseudomonas cellulosa ATCC XXXX, exhibits enhanced levels of cellulase production relative to the untransformed Pseudomonas cellulosa strain #142 ATCC 55702.
- Inventors:
-
- Knoxville, TN
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- OSTI Identifier:
- 872543
- Patent Number(s):
- 5958740
- Assignee:
- Lockheed Martin Energy Research Corp. (Oak Ridge, TN)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- AC05-96OR22464
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- genetically; enhanced; cellulase; production; pseudomonas; cellulosa; recombinant; dna; technology; strain; celllulosa; obtained; introducing; genetic; construct; comprising; heterologous; operably; connected; promoter; atcc; 55702; mutagenizing; transformants; treatment; mnng; selecting; producing; transformant; designated; xxxx; exhibits; levels; relative; untransformed; 142; cellulase producing; enhanced cellulase; recombinant dna; operably connected; exhibits enhanced; construct comprising; /435/424/
Citation Formats
Dees, H Craig. Genetically enhanced cellulase production in Pseudomonas cellulosa using recombinant DNA technology. United States: N. p., 1999.
Web.
Dees, H Craig. Genetically enhanced cellulase production in Pseudomonas cellulosa using recombinant DNA technology. United States.
Dees, H Craig. Fri .
"Genetically enhanced cellulase production in Pseudomonas cellulosa using recombinant DNA technology". United States. https://www.osti.gov/servlets/purl/872543.
@article{osti_872543,
title = {Genetically enhanced cellulase production in Pseudomonas cellulosa using recombinant DNA technology},
author = {Dees, H Craig},
abstractNote = {An enhanced strain of Pseudomonas celllulosa was obtained by introducing a recombinant genetic construct comprising a heterologous cellulase gene operably connected to a promoter into ATCC 55702, mutagenizing the transformants by treatment with MNNG, and selecting a high cellulase producing transformant. The transformant, designated Pseudomonas cellulosa ATCC XXXX, exhibits enhanced levels of cellulase production relative to the untransformed Pseudomonas cellulosa strain #142 ATCC 55702.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}
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