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Title: Microdialysis unit for molecular weight separation

Abstract

The present invention relates generally to an apparatus and method for separating high molecular weight molecules from low molecular weight molecules. More specifically, the invention relates to the use of microdialysis for removal of the salt (low molecular weight molecules) from a nucleotide sample (high molecular weight molecules) for ESI-MS analysis. The dialysis or separation performance of the present invention is improved by (1) increasing dialysis temperature thereby increasing desalting efficiency and improving spectrum quality; (2) adding piperidine and imidazole to the dialysis buffer solution and reducing charge states and further increasing detection sensitivity for DNA; (3) using low concentrations (0-2.5 mM NH4OAc) of dialysis buffer and shifting the DNA negative ions to higher charge states, producing a nearly 10-fold increase in detection sensitivity and a slightly decreased desalting efficiency, or (4) any combination of (1), (2), and (3).

Inventors:
 [1];  [1]
  1. Richland, WA
Issue Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
OSTI Identifier:
872524
Patent Number(s):
5954959
Assignee:
Battelle Memorial Institute (Richland, WA)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01D - SEPARATION
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
microdialysis; unit; molecular; weight; separation; relates; apparatus; method; separating; molecules; specifically; removal; salt; nucleotide; sample; esi-ms; analysis; dialysis; performance; improved; increasing; temperature; desalting; efficiency; improving; spectrum; quality; adding; piperidine; imidazole; buffer; solution; reducing; charge; detection; sensitivity; dna; concentrations; 0-2; nh4oac; shifting; negative; producing; nearly; 10-fold; increase; slightly; decreased; combination; detection sensitivity; molecular weight; buffer solution; weight molecules; separation performance; reducing charge; weight separation; /210/

Citation Formats

Smith, Richard D, and Liu, Chuanliang. Microdialysis unit for molecular weight separation. United States: N. p., 1999. Web.
Smith, Richard D, & Liu, Chuanliang. Microdialysis unit for molecular weight separation. United States.
Smith, Richard D, and Liu, Chuanliang. Fri . "Microdialysis unit for molecular weight separation". United States. https://www.osti.gov/servlets/purl/872524.
@article{osti_872524,
title = {Microdialysis unit for molecular weight separation},
author = {Smith, Richard D and Liu, Chuanliang},
abstractNote = {The present invention relates generally to an apparatus and method for separating high molecular weight molecules from low molecular weight molecules. More specifically, the invention relates to the use of microdialysis for removal of the salt (low molecular weight molecules) from a nucleotide sample (high molecular weight molecules) for ESI-MS analysis. The dialysis or separation performance of the present invention is improved by (1) increasing dialysis temperature thereby increasing desalting efficiency and improving spectrum quality; (2) adding piperidine and imidazole to the dialysis buffer solution and reducing charge states and further increasing detection sensitivity for DNA; (3) using low concentrations (0-2.5 mM NH4OAc) of dialysis buffer and shifting the DNA negative ions to higher charge states, producing a nearly 10-fold increase in detection sensitivity and a slightly decreased desalting efficiency, or (4) any combination of (1), (2), and (3).},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}

Patent:

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