Method for the enzymatic production of hydrogen
Abstract
The present invention is an enzymatic method for producing hydrogen comprising the steps of: a) forming a reaction mixture within a reaction vessel comprising a substrate capable of undergoing oxidation within a catabolic reaction, such as glucose, galactose, xylose, mannose, sucrose, lactose, cellulose, xylan and starch. The reaction mixture further comprises an amount of glucose dehydrogenase in an amount sufficient to catalyze the oxidation of the substrate, an amount of hydrogenase sufficient to catalyze an electron-requiring reaction wherein a stoichiometric yield of hydrogen is produced, an amount of pH buffer in an amount sufficient to provide an environment that allows the hydrogenase and the glucose dehydrogenase to retain sufficient activity for the production of hydrogen to occur and also comprising an amount of nicotinamide adenine dinucleotide phosphate sufficient to transfer electrons from the catabolic reaction to the electron-requiring reaction; b) heating the reaction mixture at a temperature sufficient for glucose dehydrogenase and the hydrogenase to retain sufficient activity and sufficient for the production of hydrogen to occur, and heating for a period of time that continues until the hydrogen is no longer produced by the reaction mixture, wherein the catabolic reaction and the electron-requiring reactions have rates of reaction dependentmore »
- Inventors:
-
- Kingston, TN
- State College, PA
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- OSTI Identifier:
- 872469
- Patent Number(s):
- 5942424
- Assignee:
- Lockheed Martin Energy Research Corporation (Oak Ridge, TN)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- AC05-96OR22464
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- method; enzymatic; production; hydrogen; producing; comprising; steps; forming; reaction; mixture; vessel; substrate; capable; undergoing; oxidation; catabolic; glucose; galactose; xylose; mannose; sucrose; lactose; cellulose; xylan; starch; comprises; amount; dehydrogenase; sufficient; catalyze; hydrogenase; electron-requiring; stoichiometric; yield; produced; ph; buffer; provide; environment; allows; retain; activity; occur; nicotinamide; adenine; dinucleotide; phosphate; transfer; electrons; heating; temperature; period; time; continues; reactions; rates; dependent; detecting; reaction mixture; temperature sufficient; reaction vessel; amount sufficient; producing hydrogen; hydrogen produced; enzymatic method; undergoing oxidation; vessel comprising; substrate capable; hydrogen comprising; /435/
Citation Formats
Woodward, Jonathan, and Mattingly, Susan M. Method for the enzymatic production of hydrogen. United States: N. p., 1999.
Web.
Woodward, Jonathan, & Mattingly, Susan M. Method for the enzymatic production of hydrogen. United States.
Woodward, Jonathan, and Mattingly, Susan M. Fri .
"Method for the enzymatic production of hydrogen". United States. https://www.osti.gov/servlets/purl/872469.
@article{osti_872469,
title = {Method for the enzymatic production of hydrogen},
author = {Woodward, Jonathan and Mattingly, Susan M},
abstractNote = {The present invention is an enzymatic method for producing hydrogen comprising the steps of: a) forming a reaction mixture within a reaction vessel comprising a substrate capable of undergoing oxidation within a catabolic reaction, such as glucose, galactose, xylose, mannose, sucrose, lactose, cellulose, xylan and starch. The reaction mixture further comprises an amount of glucose dehydrogenase in an amount sufficient to catalyze the oxidation of the substrate, an amount of hydrogenase sufficient to catalyze an electron-requiring reaction wherein a stoichiometric yield of hydrogen is produced, an amount of pH buffer in an amount sufficient to provide an environment that allows the hydrogenase and the glucose dehydrogenase to retain sufficient activity for the production of hydrogen to occur and also comprising an amount of nicotinamide adenine dinucleotide phosphate sufficient to transfer electrons from the catabolic reaction to the electron-requiring reaction; b) heating the reaction mixture at a temperature sufficient for glucose dehydrogenase and the hydrogenase to retain sufficient activity and sufficient for the production of hydrogen to occur, and heating for a period of time that continues until the hydrogen is no longer produced by the reaction mixture, wherein the catabolic reaction and the electron-requiring reactions have rates of reaction dependent upon the temperature; and c) detecting the hydrogen produced from the reaction mixture.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1999},
month = {Fri Jan 01 00:00:00 EST 1999}
}
Works referenced in this record:
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