Mutation assays involving blood cells that metabolize toxic substances
Abstract
The present invention pertains to a line of human blood cells which have high levels of oxidative activity (such as oxygenase, oxidase, peroxidase, and hydroxylase activity). Such cells grow in suspension culture, and are useful to determine the mutagenicity of xenobiotic substances that are metabolized into toxic or mutagenic substances. The invention also includes mutation assays using these cells, and other cells with similar characteristics.
- Inventors:
-
- Marblehead, MA
- Winchester, MA
- Issue Date:
- Research Org.:
- Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States)
- OSTI Identifier:
- 872440
- Patent Number(s):
- 5935829
- Assignee:
- Massachusetts Institute of Technology (Cambridge, MA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- AC04-76
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- mutation; assays; involving; blood; cells; metabolize; toxic; substances; pertains; line; human; levels; oxidative; activity; oxygenase; oxidase; peroxidase; hydroxylase; grow; suspension; culture; useful; determine; mutagenicity; xenobiotic; metabolized; mutagenic; similar; characteristics; blood cells; blood cell; mutation assays; human blood; metabolize toxic; oxidative activity; suspension culture; toxic substances; hydroxylase activity; cells grow; assays involving; involving blood; /435/
Citation Formats
Crespi, Charles L, and Thilly, William G. Mutation assays involving blood cells that metabolize toxic substances. United States: N. p., 1999.
Web.
Crespi, Charles L, & Thilly, William G. Mutation assays involving blood cells that metabolize toxic substances. United States.
Crespi, Charles L, and Thilly, William G. Fri .
"Mutation assays involving blood cells that metabolize toxic substances". United States. https://www.osti.gov/servlets/purl/872440.
@article{osti_872440,
title = {Mutation assays involving blood cells that metabolize toxic substances},
author = {Crespi, Charles L and Thilly, William G},
abstractNote = {The present invention pertains to a line of human blood cells which have high levels of oxidative activity (such as oxygenase, oxidase, peroxidase, and hydroxylase activity). Such cells grow in suspension culture, and are useful to determine the mutagenicity of xenobiotic substances that are metabolized into toxic or mutagenic substances. The invention also includes mutation assays using these cells, and other cells with similar characteristics.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}
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Works referenced in this record:
Definition of conditions for the detection of genotoxic chemicals in the adult rat-liver epithelial cell/hypoxanthine—guanine phosphoribosyl transferase (ARL/HGRPT) mutagenesis assay
journal, February 1980
- Tong, Charles; Williams, Gary M.
- Mutation Research/Environmental Mutagenesis and Related Subjects, Vol. 74, Issue 1
Differences in responses of 4 adult rat-liver epithelial cell lines to a spectrum of chemical mutagens
journal, February 1984
- Tong, Charles; Telang, Shyla; Williams, Gary M.
- Mutation Research/Environmental Mutagenesis and Related Subjects, Vol. 130, Issue 1
Induction of purine analog-resistant mutants in adult rat liver epithelial lines by metabolic activation-dependent and -independent carcinogens
journal, November 1978
- Tong, Charles; Williams, Gary M.
- Mutation Research/Genetic Toxicology, Vol. 58, Issue 2-3