Title: Enhancing the sensitivity of immunoassay procedures by use of antibodies directed to the product of a reaction between probe labels and assay substrates

Abstract

The subject invention provides an antibody which specifically binds to the product of a reaction between a labeling substance and a substrate. The subject invention also provides a method of making an immunogen used to produce the antibody of the subject invention. The invention further provides methods of using the subject antibody for detecting an antigen of interest in a sample, for example, detecting a protein comprising an amino acid sequence of interest and detecting a nucleic acid molecule comprising a nucleic acid sequence of interest, detecting a polypeptide such as those expressed by infectious agents, fungi or parasites.

Inventors:

Erlanger, Bernard F ^[1]; Chen, Bi-Xing ^[2]

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1. Whitestone, NY
2. New York, NY

Issue Date:

Fri Jan 01 00:00:00 EST 1999

Research Org.:

University of Vermont

OSTI Identifier:

872397

Patent Number(s):

5925532

Assignee:

Trustees of Columbia University in City of New York (New York, NY)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

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C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/6804 - Nucleic acid analysis using immunogens

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N33/54306 - {Solid-phase reaction mechanisms}

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DOE Contract Number:  

FG02-88ER60742

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

enhancing; sensitivity; immunoassay; procedures; antibodies; directed; product; reaction; probe; labels; assay; substrates; subject; provides; antibody; specifically; binds; labeling; substance; substrate; method; immunogen; produce; methods; detecting; antigen; sample; example; protein; comprising; amino; acid; sequence; nucleic; molecule; polypeptide; expressed; infectious; agents; fungi; parasites; acid molecule; provides methods; acid sequence; amino acid; nucleic acid; specifically bind; molecule comprising; specifically binds; immunoassay procedures; probe labels; antibodies directed; assay substrates; /435/436/