Method for nucleic acid isolation using supercritical fluids
Abstract
A method for detecting the presence of a microorganism in an environmental sample involves contacting the sample with a supercritical fluid to isolate nucleic acid from the microorganism, then detecting the presence of a particular sequence within the isolated nucleic acid. The nucleic acid may optionally be subjected to further purification.
- Inventors:
-
- 11912 Kingsgate Rd., Knoxville, TN 37911
- 3700 Sutherland Ave. #Q2, Knoxville, TN 37911
- Issue Date:
- Research Org.:
- Microbial Insight, Inc., Knoxville, TN (United States)
- OSTI Identifier:
- 872383
- Patent Number(s):
- 5922536
- Assignee:
- Nivens, David E. (11912 Kingsgate Rd., Knoxville, TN 37911);Applegate, Bruce M. (3700 Sutherland Ave. #Q2, Knoxville, TN 37911)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FG02-95ER82027
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- method; nucleic; acid; isolation; supercritical; fluids; detecting; presence; microorganism; environmental; sample; involves; contacting; fluid; isolate; particular; sequence; isolated; optionally; subjected; purification; involves contacting; environmental sample; supercritical fluid; nucleic acid; supercritical fluids; acid isolation; /435/999/
Citation Formats
Nivens, David E, and Applegate, Bruce M. Method for nucleic acid isolation using supercritical fluids. United States: N. p., 1999.
Web.
Nivens, David E, & Applegate, Bruce M. Method for nucleic acid isolation using supercritical fluids. United States.
Nivens, David E, and Applegate, Bruce M. Fri .
"Method for nucleic acid isolation using supercritical fluids". United States. https://www.osti.gov/servlets/purl/872383.
@article{osti_872383,
title = {Method for nucleic acid isolation using supercritical fluids},
author = {Nivens, David E and Applegate, Bruce M},
abstractNote = {A method for detecting the presence of a microorganism in an environmental sample involves contacting the sample with a supercritical fluid to isolate nucleic acid from the microorganism, then detecting the presence of a particular sequence within the isolated nucleic acid. The nucleic acid may optionally be subjected to further purification.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Membrane-bound DNA from Escherichia coli : Extraction by freeze-thaw-lysozyme
journal, July 1975
- Grossman, Nili; Ron, Eliora Z.
- FEBS Letters, Vol. 54, Issue 3
Detection and enumeration of bacteria in soil by direct DNA extraction and polymerase chain reaction.
journal, January 1992
- Picard, C.; Ponsonnet, C.; Paget, E.
- Applied and Environmental Microbiology, Vol. 58, Issue 9
Polymerase chain reaction-gene probe detection of microorganisms by using filter-concentrated samples.
journal, January 1991
- Bej, A. K.; Mahbubani, M. H.; Dicesare, J. L.
- Applied and Environmental Microbiology, Vol. 57, Issue 12
Detection of Escherichia coli in sewage and sludge by polymerase chain reaction.
journal, January 1993
- Tsai, Y. L.; Palmer, C. J.; Sangermano, L. R.
- Applied and Environmental Microbiology, Vol. 59, Issue 2
Recovery of DNA from soils and sediments.
journal, January 1988
- Steffan, R. J.; Goksøyr, J.; Bej, A. K.
- Applied and Environmental Microbiology, Vol. 54, Issue 12
PCR Detection of Polycyclic Aromatic Hydrocarbon-Degrading Mycobacteria
journal, January 1996
- Wang, Rong-Fu; Luneau, Allison; Cao, Wei-Wen
- Environmental Science & Technology, Vol. 30, Issue 1
Detection of coliform bacteria in water by polymerase chain reaction and gene probes.
journal, January 1990
- Bej, A. K.; Steffan, R. J.; DiCesare, J.
- Applied and Environmental Microbiology, Vol. 56, Issue 2
Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples.
journal, January 1995
- Johnson, D. W.; Pieniazek, N. J.; Griffin, D. W.
- Applied and environmental microbiology, Vol. 61, Issue 11
Simple, rapid method for direct isolation of nucleic acids from aquatic environments.
journal, January 1989
- Somerville, C. C.; Knight, I. T.; Straube, W. L.
- Applied and Environmental Microbiology, Vol. 55, Issue 3
Quantitative cell lysis of indigenous microorganisms and rapid extraction of microbial DNA from sediment.
journal, January 1994
- Moré, M. I.; Herrick, J. B.; Silva, M. C.
- Applied and Environmental Microbiology, Vol. 60, Issue 5
Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction.
journal, January 1992
- Tsai, Y. L.; Olson, B. H.
- Applied and Environmental Microbiology, Vol. 58, Issue 7
Detection of Escherichia coli and Shigella spp. in water by using the polymerase chain reaction and gene probes for uid.
journal, January 1991
- Bej, A. K.; DiCesare, J. L.; Haff, L.
- Applied and Environmental Microbiology, Vol. 57, Issue 4
The extraction and purification of microbial DNA from sediments
journal, December 1987
- Ogram, Andrew; Sayler, Gary S.; Barkay, Tamar
- Journal of Microbiological Methods, Vol. 7, Issue 2-3