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Title: Method for nucleic acid isolation using supercritical fluids

Abstract

A method for detecting the presence of a microorganism in an environmental sample involves contacting the sample with a supercritical fluid to isolate nucleic acid from the microorganism, then detecting the presence of a particular sequence within the isolated nucleic acid. The nucleic acid may optionally be subjected to further purification.

Inventors:
 [1];  [2]
  1. 11912 Kingsgate Rd., Knoxville, TN 37911
  2. 3700 Sutherland Ave. #Q2, Knoxville, TN 37911
Issue Date:
Research Org.:
Microbial Insight, Inc., Knoxville, TN (United States)
OSTI Identifier:
872383
Patent Number(s):
5922536
Assignee:
Nivens, David E. (11912 Kingsgate Rd., Knoxville, TN 37911);Applegate, Bruce M. (3700 Sutherland Ave. #Q2, Knoxville, TN 37911)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
FG02-95ER82027
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
method; nucleic; acid; isolation; supercritical; fluids; detecting; presence; microorganism; environmental; sample; involves; contacting; fluid; isolate; particular; sequence; isolated; optionally; subjected; purification; involves contacting; environmental sample; supercritical fluid; nucleic acid; supercritical fluids; acid isolation; /435/999/

Citation Formats

Nivens, David E, and Applegate, Bruce M. Method for nucleic acid isolation using supercritical fluids. United States: N. p., 1999. Web.
Nivens, David E, & Applegate, Bruce M. Method for nucleic acid isolation using supercritical fluids. United States.
Nivens, David E, and Applegate, Bruce M. Fri . "Method for nucleic acid isolation using supercritical fluids". United States. https://www.osti.gov/servlets/purl/872383.
@article{osti_872383,
title = {Method for nucleic acid isolation using supercritical fluids},
author = {Nivens, David E and Applegate, Bruce M},
abstractNote = {A method for detecting the presence of a microorganism in an environmental sample involves contacting the sample with a supercritical fluid to isolate nucleic acid from the microorganism, then detecting the presence of a particular sequence within the isolated nucleic acid. The nucleic acid may optionally be subjected to further purification.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}

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Works referenced in this record:

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