Method for nucleic acid isolation using supercritical fluids
Abstract
A method for detecting the presence of a microorganism in an environmental sample involves contacting the sample with a supercritical fluid to isolate nucleic acid from the microorganism, then detecting the presence of a particular sequence within the isolated nucleic acid. The nucleic acid may optionally be subjected to further purification.
- Inventors:
-
- 11912 Kingsgate Rd., Knoxville, TN 37911
- 3700 Sutherland Ave. #Q2, Knoxville, TN 37911
- Issue Date:
- Research Org.:
- Microbial Insight, Inc., Knoxville, TN (United States)
- OSTI Identifier:
- 872383
- Patent Number(s):
- 5922536
- Assignee:
- Nivens, David E. (11912 Kingsgate Rd., Knoxville, TN 37911);Applegate, Bruce M. (3700 Sutherland Ave. #Q2, Knoxville, TN 37911)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FG02-95ER82027
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- method; nucleic; acid; isolation; supercritical; fluids; detecting; presence; microorganism; environmental; sample; involves; contacting; fluid; isolate; particular; sequence; isolated; optionally; subjected; purification; involves contacting; environmental sample; supercritical fluid; nucleic acid; supercritical fluids; acid isolation; /435/999/
Citation Formats
Nivens, David E, and Applegate, Bruce M. Method for nucleic acid isolation using supercritical fluids. United States: N. p., 1999.
Web.
Nivens, David E, & Applegate, Bruce M. Method for nucleic acid isolation using supercritical fluids. United States.
Nivens, David E, and Applegate, Bruce M. Fri .
"Method for nucleic acid isolation using supercritical fluids". United States. https://www.osti.gov/servlets/purl/872383.
@article{osti_872383,
title = {Method for nucleic acid isolation using supercritical fluids},
author = {Nivens, David E and Applegate, Bruce M},
abstractNote = {A method for detecting the presence of a microorganism in an environmental sample involves contacting the sample with a supercritical fluid to isolate nucleic acid from the microorganism, then detecting the presence of a particular sequence within the isolated nucleic acid. The nucleic acid may optionally be subjected to further purification.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1999},
month = {Fri Jan 01 00:00:00 EST 1999}
}
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