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Title: Ethanol production in gram-positive microbes

Abstract

The subject invention concerns the transformation of Gram-positive bacteria with heterologous genes which confer upon these microbes the ability to produce ethanol as a fermentation product. Specifically exemplified is the transformation of bacteria with genes, obtainable from Zymomonas mobilis, which encode pyruvate decarboxylase and alcohol dehydrogenase.

Inventors:
 [1];  [1]
  1. Gainesville, FL
Issue Date:
Research Org.:
Univ. of Florida, Gainesville, FL (United States)
OSTI Identifier:
872357
Patent Number(s):
5916787
Assignee:
University of Florida (Gainesville, FL)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
FG05-86ER13574
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
ethanol; production; gram-positive; microbes; subject; concerns; transformation; bacteria; heterologous; genes; confer; ability; produce; fermentation; product; specifically; exemplified; obtainable; zymomonas; mobilis; encode; pyruvate; decarboxylase; alcohol; dehydrogenase; fermentation product; zymomonas mobilis; ethanol production; alcohol dehydrogenase; produce ethanol; pyruvate decarboxylase; specifically exemplified; heterologous genes; gram-positive bacteria; gram-positive microbes; /435/

Citation Formats

Ingram, Lonnie O'Neal, and Barbosa-Alleyne, Maria D. F. Ethanol production in gram-positive microbes. United States: N. p., 1999. Web.
Ingram, Lonnie O'Neal, & Barbosa-Alleyne, Maria D. F. Ethanol production in gram-positive microbes. United States.
Ingram, Lonnie O'Neal, and Barbosa-Alleyne, Maria D. F. Fri . "Ethanol production in gram-positive microbes". United States. https://www.osti.gov/servlets/purl/872357.
@article{osti_872357,
title = {Ethanol production in gram-positive microbes},
author = {Ingram, Lonnie O'Neal and Barbosa-Alleyne, Maria D. F.},
abstractNote = {The subject invention concerns the transformation of Gram-positive bacteria with heterologous genes which confer upon these microbes the ability to produce ethanol as a fermentation product. Specifically exemplified is the transformation of bacteria with genes, obtainable from Zymomonas mobilis, which encode pyruvate decarboxylase and alcohol dehydrogenase.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}

Works referenced in this record:

Production of ethanol byClostridium thermosaccharolyticum: II. A quantitative model describing product distributions
journal, December 1989


Cloning and expression of the structural gene for pyruvate decarboxylase of Zymomonas mobilis in Escherichia coli
journal, April 1986


Ethanol tolerance and carbohydrate metabolism in lactobacilli
journal, July 1992


Production of ethanol byClostridium thermosaccharolyticum: I. Effect of cell recycle and environmental parameters
journal, December 1989


Nucleotide sequence of the pyruvate decarboxylase gene from Zymomonas mobilis
journal, January 1987


Fermentation of d-Xylose to Ethanol by Genetically Modified Klebsiella planticola
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Energy and calcium ion dependence of proteolysis during sporulation of Bacillus subtilis cells.
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Establishment of a Host-vector System in Lactobacillus helveticus with β-Galactosidase Activity as a Selection Marker
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Expression of a xylanase gene of Bacillus pumilus in Escherichia coli and Bacillus subtilis
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Expression of the Escherichia coli xylose isomerase gene in Saccharomyces cerevisiae.
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Cloning of the debranching-enzyme gene from Thermoanaerobium brockii into Escherichia coli and Bacillus subtilis.
journal, January 1987


Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis.
journal, June 1987


Cloning and sequencing of the major intracellular serine protease gene of Bacillus subtilis.
journal, January 1986


Expression of theZymomonas mobilis alcohol dehydrogenase II (adhB) and pyruvate decarboxylase (pdc) genes inBacillus
journal, May 1994


Genetic engineering of soft-rot bacteria for ethanol production from lignocellulose
journal, May 1993


Fermentation of d-Xylose and l-Arabinose to Ethanol by Erwinia chrysanthemi
journal, January 1987


Pyruvate decarboxylase from Zymomonas mobilis. Isolation and partial characterization
journal, November 1986