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Title: Time-resolved fluorescence decay measurements for flowing particles

Abstract

Time-resolved fluorescence decay measurements for flowing particles. An apparatus and method for the measurement and analysis of fluorescence for individual cells and particles in flow are described, wherein the rapid measurement capabilities of flow cytometry and the robust measurement and analysis procedures of time-domain fluorescence lifetime spectroscopy are combined. A pulse-modulated cw laser is employed for excitation of the particles. The characteristics and the repetition rate of the excitation pulses can be readily adjusted to accommodate for fluorescence decays having a wide range of lifetimes.

Inventors:
 [1];  [2]
  1. Miami, FL
  2. Los Alamos, NM
Issue Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
OSTI Identifier:
872317
Patent Number(s):
5909278
Assignee:
Regents of University of California (Los Alamos, NM)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G - PHYSICS G01 - MEASURING G01J - MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRA-RED, VISIBLE OR ULTRA-VIOLET LIGHT
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
time-resolved; fluorescence; decay; measurements; flowing; particles; apparatus; method; measurement; analysis; individual; cells; flow; described; rapid; capabilities; cytometry; robust; procedures; time-domain; lifetime; spectroscopy; combined; pulse-modulated; cw; laser; employed; excitation; characteristics; repetition; rate; pulses; readily; adjusted; accommodate; decays; wide; range; lifetimes; individual cells; repetition rate; wide range; flow cytometry; individual cell; excitation pulse; fluorescence lifetime; rapid measurement; excitation pulses; time-resolved fluorescence; fluorescence decay; flowing particles; decay measurements; lifetime spectroscopy; /356/250/436/

Citation Formats

Deka, Chiranjit, and Steinkamp, John A. Time-resolved fluorescence decay measurements for flowing particles. United States: N. p., 1999. Web.
Deka, Chiranjit, & Steinkamp, John A. Time-resolved fluorescence decay measurements for flowing particles. United States.
Deka, Chiranjit, and Steinkamp, John A. Fri . "Time-resolved fluorescence decay measurements for flowing particles". United States. https://www.osti.gov/servlets/purl/872317.
@article{osti_872317,
title = {Time-resolved fluorescence decay measurements for flowing particles},
author = {Deka, Chiranjit and Steinkamp, John A},
abstractNote = {Time-resolved fluorescence decay measurements for flowing particles. An apparatus and method for the measurement and analysis of fluorescence for individual cells and particles in flow are described, wherein the rapid measurement capabilities of flow cytometry and the robust measurement and analysis procedures of time-domain fluorescence lifetime spectroscopy are combined. A pulse-modulated cw laser is employed for excitation of the particles. The characteristics and the repetition rate of the excitation pulses can be readily adjusted to accommodate for fluorescence decays having a wide range of lifetimes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}

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Works referenced in this record:

Fluorescence detection and size measurement of single DNA molecules
journal, April 1993


Time-resolved flow cytometry for the measurement of lanthanide chelate fluorescence: I. Concept and theoretical evaluation
journal, July 1994


Time-resolved fluorescence-decay measurement and analysis on single cells by flow cytometry
journal, January 1996


Time-resolved flow cytometry for the measurement of lanthanide chelate fluorescence: II. Instrument design and experimental results
journal, July 1994