Title: 5'to 3' nucleic acid synthesis using 3'-photoremovable protecting group

Abstract

The present invention relates, in general, to a method of synthesizing a nucleic acid, and, in particular, to a method of effecting 5' to 3' nucleic acid synthesis. The method can be used to prepare arrays of oligomers bound to a support via their 5' end. The invention also relates to a method of effecting mutation analysis using such arrays. The invention further relates to compounds and compositions suitable for use in such methods.

Inventors:

Pirrung, Michael C ^[1]; Shuey, Steven W ^[2]; Bradley, Jean-Claude ^[2]

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1. Houston, TX
2. Durham, NC

Issue Date:

1999-01-01

Research Org.:

Duke University (Durham, NC)

OSTI Identifier:

872315

Patent Number(s):

5908926

Assignee:

Duke University (Durham, NC)

Patent Classifications (CPCs):

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02P - CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS

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Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02P - CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS

Y02P20/55 - Synthetic design, e.g. reducing the use of auxiliary or protecting groups

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS

C07H19/10 - with the saccharide radical esterified by phosphoric or polyphosphoric acids
C07H21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
C07H19/20 - with the saccharide radical esterified by phosphoric or polyphosphoric acids

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DOE Contract Number:  

FG05-92ER61388

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

nucleic; acid; synthesis; -photoremovable; protecting; relates; method; synthesizing; particular; effecting; prepare; arrays; oligomers; bound; support; via; mutation; analysis; compounds; compositions; suitable; methods; nucleic acid; compositions suitable; acid synthesis; /999/