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Title: 5'to 3' nucleic acid synthesis using 3'-photoremovable protecting group

Abstract

The present invention relates, in general, to a method of synthesizing a nucleic acid, and, in particular, to a method of effecting 5' to 3' nucleic acid synthesis. The method can be used to prepare arrays of oligomers bound to a support via their 5' end. The invention also relates to a method of effecting mutation analysis using such arrays. The invention further relates to compounds and compositions suitable for use in such methods.

Inventors:
 [1];  [2];  [2]
  1. Houston, TX
  2. Durham, NC
Issue Date:
Research Org.:
Duke University (Durham, NC)
OSTI Identifier:
872315
Patent Number(s):
5908926
Assignee:
Duke University (Durham, NC)
Patent Classifications (CPCs):
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02P - CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS
DOE Contract Number:  
FG05-92ER61388
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
nucleic; acid; synthesis; -photoremovable; protecting; relates; method; synthesizing; particular; effecting; prepare; arrays; oligomers; bound; support; via; mutation; analysis; compounds; compositions; suitable; methods; nucleic acid; compositions suitable; acid synthesis; /999/

Citation Formats

Pirrung, Michael C, Shuey, Steven W, and Bradley, Jean-Claude. 5'to 3' nucleic acid synthesis using 3'-photoremovable protecting group. United States: N. p., 1999. Web.
Pirrung, Michael C, Shuey, Steven W, & Bradley, Jean-Claude. 5'to 3' nucleic acid synthesis using 3'-photoremovable protecting group. United States.
Pirrung, Michael C, Shuey, Steven W, and Bradley, Jean-Claude. Fri . "5'to 3' nucleic acid synthesis using 3'-photoremovable protecting group". United States. https://www.osti.gov/servlets/purl/872315.
@article{osti_872315,
title = {5'to 3' nucleic acid synthesis using 3'-photoremovable protecting group},
author = {Pirrung, Michael C and Shuey, Steven W and Bradley, Jean-Claude},
abstractNote = {The present invention relates, in general, to a method of synthesizing a nucleic acid, and, in particular, to a method of effecting 5' to 3' nucleic acid synthesis. The method can be used to prepare arrays of oligomers bound to a support via their 5' end. The invention also relates to a method of effecting mutation analysis using such arrays. The invention further relates to compounds and compositions suitable for use in such methods.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}

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Works referenced in this record:

Light-directed, spatially addressable parallel chemical synthesis
journal, February 1991


Comparison of Methods for Photochemical Phosphoramidite-Based DNA Synthesis
journal, October 1995


Dimethoxybenzoin Carbonates: Photochemically-Removable Alcohol Protecting Groups Suitable for Phosphoramidite-Based DNA Synthesis
journal, March 1995


Photochemically-removable silyl protecting groups
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Multiplexed biochemical assays with biological chips
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Recognition of mixed-sequence duplex DNA by alternate-strand triple-helix formation
journal, March 1990


Photochemical protodesilylation of 2-R3Si-1,3-dimethoxybenzenes. Direct observation of .beta.-silyl-substituted cyclohexadienyl cations
journal, December 1993


Photochemistry of phosphate esters
journal, January 1993