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Title: Use of continuous/contiguous stacking hybridization as a diagnostic tool

Abstract

A method for detecting disease-associated alleles in patient genetic material is provided whereby a first group of oligonucleotide molecules, synthesized to compliment base sequences of the disease associated alleles is immobilized on a predetermined position on a substrate, and then contacted with patient genetic material to form duplexes. The duplexes are then contacted with a second group of oligonucleotide molecules which are synthesized to extend the predetermined length of the oligonucleotide molecules of the first group, and where each of the oligonucleotide molecules of the second group are tagged and either incorporate universal bases or a mixture of guanine, cytosine, thymine, and adenine, or complementary nucleotide strands that are tagged with a different fluorochrome which radiates light at a predetermined wavelength. The treated substrate is then washed and the light patterns radiating therefrom are compared with predetermined light patterns of various diseases that were prepared on identical substrates.

Inventors:
 [1];  [2];  [1];  [1];  [1];  [1]
+ Show Inventor Affiliations
  1. Moscow, RU
  2. Zelenograd, RU
Issue Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL (United States)
OSTI Identifier:
872311
Patent Number(s):
5908745
Assignee:
University of Chicago (Chicago, IL)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
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DOE Contract Number:  
W-31109-ENG-38
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
continuous; contiguous; stacking; hybridization; diagnostic; tool; method; detecting; disease-associated; alleles; patient; genetic; material; provided; whereby; oligonucleotide; molecules; synthesized; compliment; base; sequences; disease; associated; immobilized; predetermined; position; substrate; contacted; form; duplexes; extend; length; tagged; incorporate; universal; bases; mixture; guanine; cytosine; thymine; adenine; complementary; nucleotide; strands; fluorochrome; radiates; light; wavelength; treated; washed; patterns; radiating; therefrom; compared; various; diseases; prepared; identical; substrates; various diseases; predetermined position; light pattern; base sequences; predetermined wavelength; genetic material; provided whereby; oligonucleotide molecules; base sequence; light patterns; complementary nucleotide; patient genetic; nucleotide strand; nucleotide strands; compliment base; diagnostic tool; treated substrate; stacking hybridization; associated alleles; detecting disease-associated; disease-associated alleles; contiguous stacking; /435/436/

Citation Formats

Mirzabekov, Andrei Darievich, Yershov, Gennadiy Moseyevich, Kirillov, Eugene Vladislavovich, Parinov, Sergei Valeryevich, Barski, Victor Evgenievich, and Lysov, Yuri Petrovich. Use of continuous/contiguous stacking hybridization as a diagnostic tool. United States: N. p., 1999. Web.
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Mirzabekov, Andrei Darievich, Yershov, Gennadiy Moseyevich, Kirillov, Eugene Vladislavovich, Parinov, Sergei Valeryevich, Barski, Victor Evgenievich, & Lysov, Yuri Petrovich. Use of continuous/contiguous stacking hybridization as a diagnostic tool. United States.
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Mirzabekov, Andrei Darievich, Yershov, Gennadiy Moseyevich, Kirillov, Eugene Vladislavovich, Parinov, Sergei Valeryevich, Barski, Victor Evgenievich, and Lysov, Yuri Petrovich. Fri . "Use of continuous/contiguous stacking hybridization as a diagnostic tool". United States. https://www.osti.gov/servlets/purl/872311.
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@article{osti_872311,
title = {Use of continuous/contiguous stacking hybridization as a diagnostic tool},
author = {Mirzabekov, Andrei Darievich and Yershov, Gennadiy Moseyevich and Kirillov, Eugene Vladislavovich and Parinov, Sergei Valeryevich and Barski, Victor Evgenievich and Lysov, Yuri Petrovich},
abstractNote = {A method for detecting disease-associated alleles in patient genetic material is provided whereby a first group of oligonucleotide molecules, synthesized to compliment base sequences of the disease associated alleles is immobilized on a predetermined position on a substrate, and then contacted with patient genetic material to form duplexes. The duplexes are then contacted with a second group of oligonucleotide molecules which are synthesized to extend the predetermined length of the oligonucleotide molecules of the first group, and where each of the oligonucleotide molecules of the second group are tagged and either incorporate universal bases or a mixture of guanine, cytosine, thymine, and adenine, or complementary nucleotide strands that are tagged with a different fluorochrome which radiates light at a predetermined wavelength. The treated substrate is then washed and the light patterns radiating therefrom are compared with predetermined light patterns of various diseases that were prepared on identical substrates.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1999},
month = {Fri Jan 01 00:00:00 EST 1999}
}
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Works referenced in this record:

A method for DNA sequencing by hybridization with oligonucleotide matrix
journal, January 1991

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