cDNA encoding a polypeptide including a hevein sequence
Abstract
A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74-79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli.
- Inventors:
-
- Okemos, MI
- Dilbeek, BE
- Scarsdale, NY
- New York, NY
- Issue Date:
- Research Org.:
- Michigan State Univ., East Lansing, MI (United States)
- OSTI Identifier:
- 872273
- Patent Number(s):
- 5900480
- Application Number:
- 07/888,366
- Assignee:
- Board of Trustees operating Michigan State University (East Lansing, MI)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
- DOE Contract Number:
- AC02-76ER01338
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- cdna; encoding; polypeptide; including; hevein; sequence; clone; hev1; isolated; via; polymerase; chain; reaction; pcr; mixed; oligonucleotides; corresponding; regions; primers; hevea; brasiliensis; latex; library; template; 1018; nucleotides; reading; frame; 204; amino; acids; deduced; acid; contains; putative; signal; 17; residues; followed; 187; amino-terminal; region; 43; identical; homology; chitin-binding; proteins; amino-termini; wound-induced; genes; potato; poplar; carboxyl-terminal; portion; 144; 74-79; homologous; wound-inducible; wounding; application; plant; hormones; abscisic; ethylene; resulted; accumulation; transcripts; leaves; stems; roots; shown; probe; fusion; protein; produced; coli; maltose; binding; cdna clone; deduced amino; reading frame; chain reaction; binding protein; acid sequence; amino acid; polymerase chain; amino acids; cdna library; dna encoding; cdna encoding; residues followed; protein produced; putative signal; wound-induced genes; wound-inducible genes; abscisic acid; carboxyl-terminal portion; carboxyl-terminal region; maltose binding; plant hormones; chitin-binding proteins; amino-terminal region; sequence contains; signal sequence; oligonucleotides corresponding; mixed oligonucleotides; via polymerase; encoding hevein; hevea brasiliensis; acid residues; acid residue; acid polypeptide; brasiliensis latex; latex cdna; isolated via; polypeptide including; fusion protein; hormones abscisic; hevein sequence; hevein transcripts; nucleotides corresponding; /999/435/
Citation Formats
Raikhel, Natasha V, Broekaert, Willem F, Chua, Nam-Hai, and Kush, Anil. cDNA encoding a polypeptide including a hevein sequence. United States: N. p., 1999.
Web.
Raikhel, Natasha V, Broekaert, Willem F, Chua, Nam-Hai, & Kush, Anil. cDNA encoding a polypeptide including a hevein sequence. United States.
Raikhel, Natasha V, Broekaert, Willem F, Chua, Nam-Hai, and Kush, Anil. Tue .
"cDNA encoding a polypeptide including a hevein sequence". United States. https://www.osti.gov/servlets/purl/872273.
@article{osti_872273,
title = {cDNA encoding a polypeptide including a hevein sequence},
author = {Raikhel, Natasha V and Broekaert, Willem F and Chua, Nam-Hai and Kush, Anil},
abstractNote = {A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74-79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {5}
}
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