Geranyl diphosphate synthase from mint
Abstract
A cDNA encoding geranyl diphosphate synthase from peppermint has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID No:1) is provided which codes for the expression of geranyl diphosphate synthase (SEQ ID No:2) from peppermint (Mentha piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for geranyl diphosphate synthase or for a base sequence sufficiently complementary to at least a portion of the geranyl diphosphate synthase DNA or RNA to enable hybridization therewith (e.g., antisense geranyl diphosphate synthase RNA or fragments of complementary geranyl diphosphate synthase DNA which are useful as polymerase chain reaction primers or as probes for geranyl diphosphate synthase or related genes). In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding geranyl diphosphate synthase. Thus, systems and methods are provided for the recombinant expression of geranyl diphosphate synthase that may be used to facilitate the production, isolation and purification of significant quantities of recombinant geranyl diphosphate synthase for subsequent use, to obtain expression or enhanced expression of geranyl diphosphate synthase in plants in order to enhancemore »
- Inventors:
-
- Pullman, WA
- Colfax, WA
- Moscow, ID
- Jena, DE
- Issue Date:
- Research Org.:
- Washington State University Research Foundation (Pullman, WA)
- OSTI Identifier:
- 872170
- Patent Number(s):
- 5876964
- Assignee:
- Washington State University Research Foundation (Pullman, WA)
- Patent Classifications (CPCs):
-
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG03-96ER20212
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- geranyl; diphosphate; synthase; mint; cdna; encoding; peppermint; isolated; sequenced; corresponding; amino; acid; sequence; determined; accordingly; dna; seq; provided; codes; expression; mentha; piperita; aspects; replicable; recombinant; cloning; vehicles; code; base; sufficiently; complementary; portion; rna; enable; hybridization; therewith; antisense; fragments; useful; polymerase; chain; reaction; primers; probes; related; genes; modified; host; cells; transformed; transfected; infected; injected; vehicle; systems; methods; facilitate; production; isolation; purification; significant; quantities; subsequent; obtain; enhanced; plants; enhance; monoterpenoids; produce; cancerous; precursor; anti-cancer; properties; otherwise; employed; regulation; sequence sufficiently; modified host; cloning vehicles; recombinant expression; replicable recombinant; obtain expression; otherwise employed; enable hybridization; hybridization therewith; synthase dna; recombinant cloning; geranyl diphosphate; chain reaction; dna sequence; isolated dna; acid sequence; amino acid; diphosphate synthase; host cells; polymerase chain; sequence encoding; base sequence; cloning vehicle; dna encoding; host cell; sufficiently complementary; cdna encoding; mentha piperita; corresponding amino; enhanced expression; significant quantities; /435/999/
Citation Formats
Croteau, Rodney Bruce, Wildung, Mark Raymond, Burke, Charles Cullen, and Gershenzon, Jonathan. Geranyl diphosphate synthase from mint. United States: N. p., 1999.
Web.
Croteau, Rodney Bruce, Wildung, Mark Raymond, Burke, Charles Cullen, & Gershenzon, Jonathan. Geranyl diphosphate synthase from mint. United States.
Croteau, Rodney Bruce, Wildung, Mark Raymond, Burke, Charles Cullen, and Gershenzon, Jonathan. Fri .
"Geranyl diphosphate synthase from mint". United States. https://www.osti.gov/servlets/purl/872170.
@article{osti_872170,
title = {Geranyl diphosphate synthase from mint},
author = {Croteau, Rodney Bruce and Wildung, Mark Raymond and Burke, Charles Cullen and Gershenzon, Jonathan},
abstractNote = {A cDNA encoding geranyl diphosphate synthase from peppermint has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID No:1) is provided which codes for the expression of geranyl diphosphate synthase (SEQ ID No:2) from peppermint (Mentha piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for geranyl diphosphate synthase or for a base sequence sufficiently complementary to at least a portion of the geranyl diphosphate synthase DNA or RNA to enable hybridization therewith (e.g., antisense geranyl diphosphate synthase RNA or fragments of complementary geranyl diphosphate synthase DNA which are useful as polymerase chain reaction primers or as probes for geranyl diphosphate synthase or related genes). In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding geranyl diphosphate synthase. Thus, systems and methods are provided for the recombinant expression of geranyl diphosphate synthase that may be used to facilitate the production, isolation and purification of significant quantities of recombinant geranyl diphosphate synthase for subsequent use, to obtain expression or enhanced expression of geranyl diphosphate synthase in plants in order to enhance the production of monoterpenoids, to produce geranyl diphosphate in cancerous cells as a precursor to monoterpenoids having anti-cancer properties or may be otherwise employed for the regulation or expression of geranyl diphosphate synthase or the production of geranyl diphosphate.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}
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