Process for selection of Oxygen-tolerant algal mutants that produce H.sub.2
Abstract
A process for selection of oxygen-tolerant, H.sub.2 -producing algal mutant cells comprising: (a) growing algal cells photoautotrophically under fluorescent light to mid log phase; (b) inducing algal cells grown photoautrophically under fluorescent light to mid log phase in step (a) anaerobically by (1) resuspending the cells in a buffer solution and making said suspension anaerobic with an inert gas; (2) incubating the suspension in the absence of light at ambient temperature; (c) treating the cells from step (b) with metronidazole, sodium azide, and added oxygen to controlled concentrations in the presence of white light. (d) washing off metronidazole and sodium azide to obtain final cell suspension; (e) plating said final cell suspension on a minimal medium and incubating in light at a temperature sufficient to enable colonies to appear; (f) counting the number of colonies to determine the percent of mutant survivors; and (g) testing survivors to identify oxygen-tolerant H.sub.2 -producing mutants.
- Inventors:
-
- Lakewood, CO
- Issue Date:
- Research Org.:
- Midwest Research Institute, Kansas City, MO (United States)
- OSTI Identifier:
- 872155
- Patent Number(s):
- 5871952
- Assignee:
- Midwest Research Institute (Kansas City, MO)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC36-83CH10093
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- process; selection; oxygen-tolerant; algal; mutants; produce; -producing; mutant; cells; comprising; growing; photoautotrophically; fluorescent; light; mid; log; phase; inducing; grown; photoautrophically; step; anaerobically; resuspending; buffer; solution; suspension; anaerobic; inert; gas; incubating; absence; ambient; temperature; treating; metronidazole; sodium; azide; added; oxygen; controlled; concentrations; presence; white; washing; obtain; final; cell; plating; minimal; medium; sufficient; enable; colonies; appear; counting; determine; percent; survivors; testing; identify; fluorescent light; inert gas; ambient temperature; temperature sufficient; white light; buffer solution; mutant cells; algal mutant; cells grown; cells grow; cells comprising; /435/
Citation Formats
Ghirardi, Maria L, and Seibert, Michael. Process for selection of Oxygen-tolerant algal mutants that produce H.sub.2. United States: N. p., 1999.
Web.
Ghirardi, Maria L, & Seibert, Michael. Process for selection of Oxygen-tolerant algal mutants that produce H.sub.2. United States.
Ghirardi, Maria L, and Seibert, Michael. Fri .
"Process for selection of Oxygen-tolerant algal mutants that produce H.sub.2". United States. https://www.osti.gov/servlets/purl/872155.
@article{osti_872155,
title = {Process for selection of Oxygen-tolerant algal mutants that produce H.sub.2},
author = {Ghirardi, Maria L and Seibert, Michael},
abstractNote = {A process for selection of oxygen-tolerant, H.sub.2 -producing algal mutant cells comprising: (a) growing algal cells photoautotrophically under fluorescent light to mid log phase; (b) inducing algal cells grown photoautrophically under fluorescent light to mid log phase in step (a) anaerobically by (1) resuspending the cells in a buffer solution and making said suspension anaerobic with an inert gas; (2) incubating the suspension in the absence of light at ambient temperature; (c) treating the cells from step (b) with metronidazole, sodium azide, and added oxygen to controlled concentrations in the presence of white light. (d) washing off metronidazole and sodium azide to obtain final cell suspension; (e) plating said final cell suspension on a minimal medium and incubating in light at a temperature sufficient to enable colonies to appear; (f) counting the number of colonies to determine the percent of mutant survivors; and (g) testing survivors to identify oxygen-tolerant H.sub.2 -producing mutants.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}
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