Method for the production of dicarboxylic acids
Abstract
The present invention is an economical fermentation method for the production of carboxylic acids comprising the steps of a) inoculating a medium having a carbon source with a carboxylic acid-producing organism; b) incubating the carboxylic acid-producing organism in an aerobic atmosphere to promote rapid growth of the organism thereby increasing the biomass of the organism; c) controllably releasing oxygen to maintain the aerobic atmosphere; d) controllably feeding the organism having increased biomass with a solution containing the carbon source to maintain the concentration of the carbon source within the medium of about 0.5 g/L up to about 1 g/L; e) depriving the aerobic atmosphere of oxygen to produce an anaerobic atmosphere to cause the organism to undergo anaerobic metabolism; f) controllably feeding the organism having increased biomass a solution containing the carbon source to maintain the concentration of the carbon source within the medium of .gtoreq.1 g/L; and g) converting the carbon source to carboxylic acids using the anaerobic metabolism of the organism.
- Inventors:
-
- Knoxville, TN
- Warrenville, IL
- Plainfield, IL
- Woodridge, IL
- Issue Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 872139
- Patent Number(s):
- 5869301
- Assignee:
- Lockhead Martin Energy Research Corporation (Oak Ridge, TN)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- W-31109-ENG-38; AC05-96OR22464
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- method; production; dicarboxylic; acids; economical; fermentation; carboxylic; comprising; steps; inoculating; medium; carbon; source; acid-producing; organism; incubating; aerobic; atmosphere; promote; rapid; growth; increasing; biomass; controllably; releasing; oxygen; maintain; feeding; increased; solution; containing; concentration; depriving; produce; anaerobic; undergo; metabolism; gtoreq; converting; solution containing; carbon source; carboxylic acids; carboxylic acid; releasing oxygen; dicarboxylic acid; fermentation method; dicarboxylic acids; /435/
Citation Formats
Nghiem, Nhuan Phu, Donnelly, Mark, Millard, Cynthia S, and Stols, Lucy. Method for the production of dicarboxylic acids. United States: N. p., 1999.
Web.
Nghiem, Nhuan Phu, Donnelly, Mark, Millard, Cynthia S, & Stols, Lucy. Method for the production of dicarboxylic acids. United States.
Nghiem, Nhuan Phu, Donnelly, Mark, Millard, Cynthia S, and Stols, Lucy. Fri .
"Method for the production of dicarboxylic acids". United States. https://www.osti.gov/servlets/purl/872139.
@article{osti_872139,
title = {Method for the production of dicarboxylic acids},
author = {Nghiem, Nhuan Phu and Donnelly, Mark and Millard, Cynthia S and Stols, Lucy},
abstractNote = {The present invention is an economical fermentation method for the production of carboxylic acids comprising the steps of a) inoculating a medium having a carbon source with a carboxylic acid-producing organism; b) incubating the carboxylic acid-producing organism in an aerobic atmosphere to promote rapid growth of the organism thereby increasing the biomass of the organism; c) controllably releasing oxygen to maintain the aerobic atmosphere; d) controllably feeding the organism having increased biomass with a solution containing the carbon source to maintain the concentration of the carbon source within the medium of about 0.5 g/L up to about 1 g/L; e) depriving the aerobic atmosphere of oxygen to produce an anaerobic atmosphere to cause the organism to undergo anaerobic metabolism; f) controllably feeding the organism having increased biomass a solution containing the carbon source to maintain the concentration of the carbon source within the medium of .gtoreq.1 g/L; and g) converting the carbon source to carboxylic acids using the anaerobic metabolism of the organism.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}
Works referenced in this record:
Carbon metabolism regulates expression of the pfl (pyruvate formate-lyase) gene in Escherichia coli.
journal, January 1991
- Rasmussen, L. J.; Møller, P. L.; Atlung, T.
- Journal of Bacteriology, Vol. 173, Issue 20