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Title: Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit epsilon N-methyltransferase

Abstract

The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) .sup..epsilon. N-methyltransferase (protein methylase III or Rubisco LSMT) is disclosed. This enzyme catalyzes methylation of the .epsilon.-amine of lysine-14 in the large subunit of Rubisco. In addition, a full-length cDNA clone for Rubisco LSMT is disclosed. Transgenic plants and methods of producing same which (1) have the Rubisco LSMT gene inserted into the DNA, and (2) have the Rubisco LSMT gene product or the action of the gene product deleted from the DNA are also provided. Further, methods of using the gene to selectively deliver desired agents to a plant are also disclosed.

Inventors:
 [1]
  1. Lexington, KY
Issue Date:
Research Org.:
University Of Kentucky (Lexington, KY)
OSTI Identifier:
872126
Patent Number(s):
5866394
Assignee:
University of Kentucky Research Foundation (Lexington, KY)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02A - TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
DOE Contract Number:  
FG05-92ER20075
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
cloning; developmental; expression; pea; ribulose-1; 5-bisphosphate; carboxylase; oxygenase; subunit; epsilon; n-methyltransferase; sequence; rubisco; protein; methylase; iii; lsmt; disclosed; enzyme; catalyzes; methylation; -amine; lysine-14; addition; full-length; cdna; clone; transgenic; plants; methods; producing; inserted; dna; product; action; deleted; provided; selectively; deliver; desired; agents; plant; cdna clone; rubisco lsmt; transgenic plants; protein methylase; methylase iii; full-length cdna; pea ribulose-1; enzyme catalyzes; transgenic plant; developmental expression; /435/999/

Citation Formats

Houtz, Robert L. Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit epsilon N-methyltransferase. United States: N. p., 1999. Web.
Houtz, Robert L. Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit epsilon N-methyltransferase. United States.
Houtz, Robert L. Fri . "Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit epsilon N-methyltransferase". United States. https://www.osti.gov/servlets/purl/872126.
@article{osti_872126,
title = {Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit epsilon N-methyltransferase},
author = {Houtz, Robert L},
abstractNote = {The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) .sup..epsilon. N-methyltransferase (protein methylase III or Rubisco LSMT) is disclosed. This enzyme catalyzes methylation of the .epsilon.-amine of lysine-14 in the large subunit of Rubisco. In addition, a full-length cDNA clone for Rubisco LSMT is disclosed. Transgenic plants and methods of producing same which (1) have the Rubisco LSMT gene inserted into the DNA, and (2) have the Rubisco LSMT gene product or the action of the gene product deleted from the DNA are also provided. Further, methods of using the gene to selectively deliver desired agents to a plant are also disclosed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}

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Works referenced in this record:

Affinity Purification of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Large Subunit ϵN-Methyltransferase
journal, August 1995


Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes
journal, August 1988


Post-translational modifications in the large subunit of ribulose bisphosphate carboxylase/oxygenase.
journal, March 1989


Light-regulated methylation of chloroplast proteins.
journal, July 1987


Cloning and developmental expression of the sucrose-phosphate-synthase gene from spinach
journal, June 1993


Partial Purification and Characterization of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Large Subunit ε N -Methyltransferase
journal, November 1991


Protein Methylation in Pea Chloroplasts
journal, July 1990