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Title: Rapid method to detect duplex formation in sequencing by hybridization methods

Abstract

A method for determining the existence of duplexes of oligonucleotide complementary molecules is provided whereby a plurality of immobilized oligonucleotide molecules, each of a specific length and each having a specific base sequence, is contacted with complementary, single stranded oligonucleotide molecules to form a duplex so as to facilitate intercalation of a fluorescent dye between the base planes of the duplex. The invention also provides for a method for constructing oligonucleotide matrices comprising confining light sensitive fluid to a surface, exposing said light-sensitive fluid to a light pattern so as to cause the fluid exposed to the light to coalesce into discrete units and adhere to the surface; and contacting each of the units with a set of different oligonucleotide molecules so as to allow the molecules to disperse into the units.

Inventors:
 [1];  [1];  [1];  [1]
  1. (Moscow, RU)
Issue Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL
OSTI Identifier:
872104
Patent Number(s):
5861247
Assignee:
University of Chicago () ANL
DOE Contract Number:  
W-31109-ENG-38
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
rapid; method; detect; duplex; formation; sequencing; hybridization; methods; determining; existence; duplexes; oligonucleotide; complementary; molecules; provided; whereby; plurality; immobilized; specific; length; base; sequence; contacted; single; stranded; form; facilitate; intercalation; fluorescent; dye; planes; provides; constructing; matrices; comprising; confining; light; sensitive; fluid; surface; exposing; light-sensitive; pattern; exposed; coalesce; discrete; units; adhere; contacting; set; allow; disperse; single stranded; hybridization methods; light pattern; provided whereby; oligonucleotide molecules; base sequence; light sensitive; fluorescent dye; rapid method; specific base; single strand; /435/

Citation Formats

Mirzabekov, Andrei Darievich, Timofeev, Edward Nikolaevich, Florentiev, Vladimer Leonidovich, and Kirillov, Eugene Vladislavovich. Rapid method to detect duplex formation in sequencing by hybridization methods. United States: N. p., 1999. Web.
Mirzabekov, Andrei Darievich, Timofeev, Edward Nikolaevich, Florentiev, Vladimer Leonidovich, & Kirillov, Eugene Vladislavovich. Rapid method to detect duplex formation in sequencing by hybridization methods. United States.
Mirzabekov, Andrei Darievich, Timofeev, Edward Nikolaevich, Florentiev, Vladimer Leonidovich, and Kirillov, Eugene Vladislavovich. Fri . "Rapid method to detect duplex formation in sequencing by hybridization methods". United States. https://www.osti.gov/servlets/purl/872104.
@article{osti_872104,
title = {Rapid method to detect duplex formation in sequencing by hybridization methods},
author = {Mirzabekov, Andrei Darievich and Timofeev, Edward Nikolaevich and Florentiev, Vladimer Leonidovich and Kirillov, Eugene Vladislavovich},
abstractNote = {A method for determining the existence of duplexes of oligonucleotide complementary molecules is provided whereby a plurality of immobilized oligonucleotide molecules, each of a specific length and each having a specific base sequence, is contacted with complementary, single stranded oligonucleotide molecules to form a duplex so as to facilitate intercalation of a fluorescent dye between the base planes of the duplex. The invention also provides for a method for constructing oligonucleotide matrices comprising confining light sensitive fluid to a surface, exposing said light-sensitive fluid to a light pattern so as to cause the fluid exposed to the light to coalesce into discrete units and adhere to the surface; and contacting each of the units with a set of different oligonucleotide molecules so as to allow the molecules to disperse into the units.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}

Patent:

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