Rapid method to detect duplex formation in sequencing by hybridization methods
Abstract
A method for determining the existence of duplexes of oligonucleotide complementary molecules is provided whereby a plurality of immobilized oligonucleotide molecules, each of a specific length and each having a specific base sequence, is contacted with complementary, single stranded oligonucleotide molecules to form a duplex so as to facilitate intercalation of a fluorescent dye between the base planes of the duplex. The invention also provides for a method for constructing oligonucleotide matrices comprising confining light sensitive fluid to a surface, exposing said light-sensitive fluid to a light pattern so as to cause the fluid exposed to the light to coalesce into discrete units and adhere to the surface; and contacting each of the units with a set of different oligonucleotide molecules so as to allow the molecules to disperse into the units.
- Inventors:
-
- Moscow, RU
- Issue Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- OSTI Identifier:
- 872104
- Patent Number(s):
- 5861247
- Assignee:
- University of Chicago ()
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES
- DOE Contract Number:
- W-31109-ENG-38
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- rapid; method; detect; duplex; formation; sequencing; hybridization; methods; determining; existence; duplexes; oligonucleotide; complementary; molecules; provided; whereby; plurality; immobilized; specific; length; base; sequence; contacted; single; stranded; form; facilitate; intercalation; fluorescent; dye; planes; provides; constructing; matrices; comprising; confining; light; sensitive; fluid; surface; exposing; light-sensitive; pattern; exposed; coalesce; discrete; units; adhere; contacting; set; allow; disperse; single stranded; hybridization methods; light pattern; provided whereby; oligonucleotide molecules; base sequence; light sensitive; fluorescent dye; rapid method; specific base; single strand; /435/
Citation Formats
Mirzabekov, Andrei Darievich, Timofeev, Edward Nikolaevich, Florentiev, Vladimer Leonidovich, and Kirillov, Eugene Vladislavovich. Rapid method to detect duplex formation in sequencing by hybridization methods. United States: N. p., 1999.
Web.
Mirzabekov, Andrei Darievich, Timofeev, Edward Nikolaevich, Florentiev, Vladimer Leonidovich, & Kirillov, Eugene Vladislavovich. Rapid method to detect duplex formation in sequencing by hybridization methods. United States.
Mirzabekov, Andrei Darievich, Timofeev, Edward Nikolaevich, Florentiev, Vladimer Leonidovich, and Kirillov, Eugene Vladislavovich. Fri .
"Rapid method to detect duplex formation in sequencing by hybridization methods". United States. https://www.osti.gov/servlets/purl/872104.
@article{osti_872104,
title = {Rapid method to detect duplex formation in sequencing by hybridization methods},
author = {Mirzabekov, Andrei Darievich and Timofeev, Edward Nikolaevich and Florentiev, Vladimer Leonidovich and Kirillov, Eugene Vladislavovich},
abstractNote = {A method for determining the existence of duplexes of oligonucleotide complementary molecules is provided whereby a plurality of immobilized oligonucleotide molecules, each of a specific length and each having a specific base sequence, is contacted with complementary, single stranded oligonucleotide molecules to form a duplex so as to facilitate intercalation of a fluorescent dye between the base planes of the duplex. The invention also provides for a method for constructing oligonucleotide matrices comprising confining light sensitive fluid to a surface, exposing said light-sensitive fluid to a light pattern so as to cause the fluid exposed to the light to coalesce into discrete units and adhere to the surface; and contacting each of the units with a set of different oligonucleotide molecules so as to allow the molecules to disperse into the units.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1999},
month = {Fri Jan 01 00:00:00 EST 1999}
}
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Works referenced in this record:
Analyzing and comparing nucleic acid sequences by hybridization to arrays of oligonucleotides: Evaluation using experimental models
journal, August 1992
- Southern, E. M.; Maskos, U.; Elder, J. K.
- Genomics, Vol. 13, Issue 4, p. 1008-1017