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Title: Structurally stable gel bead containing entrapped enzyme and method for manufacture thereof

Abstract

A structurally stable gel bead containing an entrapped enzyme and a method for its manufacture. The enzyme is covalently cross-linked to gelatin in the presence of glutaraldehyde prior to the formation of the gel bead, to prevent leakage of the enzyme. Propylene glycol alginate is then added to the mixture. Once the gel beads are formed, they are then soaked in glutaraldehyde, which imparts structural stability to the gel beads. This method can be used with many types of enzymes, such as proteases, carbohydrases, proteases, ligases, isomerases, oxidoreductases, and specialty enzymes. These and other enzymes can be immobilized in the gel beads and utilized in a number of enzymatic processes. Exogenously added ions are not required to maintain the structural stability of these gel beads.

Inventors:
 [1]
  1. (Oak Ridge, TN)
Issue Date:
Research Org.:
LOCKHEED MARTIN ENRGY SYST INC
OSTI Identifier:
872026
Patent Number(s):
5846762
Assignee:
Lockheed Martin Energy Research Systems, Inc. (Oak Ridge, TN) ORNL
DOE Contract Number:  
AC05-84OR21400
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
structurally; stable; bead; containing; entrapped; enzyme; method; manufacture; covalently; cross-linked; gelatin; presence; glutaraldehyde; prior; formation; prevent; leakage; propylene; glycol; alginate; added; mixture; beads; formed; soaked; imparts; structural; stability; types; enzymes; proteases; carbohydrases; ligases; isomerases; oxidoreductases; specialty; immobilized; utilized; enzymatic; processes; exogenously; required; maintain; propylene glycol; prevent leakage; bead containing; structurally stable; structural stability; containing entrapped; glycol alginate; entrapped enzyme; /435/264/427/428/

Citation Formats

Woodward, Jonathan. Structurally stable gel bead containing entrapped enzyme and method for manufacture thereof. United States: N. p., 1998. Web.
Woodward, Jonathan. Structurally stable gel bead containing entrapped enzyme and method for manufacture thereof. United States.
Woodward, Jonathan. Thu . "Structurally stable gel bead containing entrapped enzyme and method for manufacture thereof". United States. https://www.osti.gov/servlets/purl/872026.
@article{osti_872026,
title = {Structurally stable gel bead containing entrapped enzyme and method for manufacture thereof},
author = {Woodward, Jonathan},
abstractNote = {A structurally stable gel bead containing an entrapped enzyme and a method for its manufacture. The enzyme is covalently cross-linked to gelatin in the presence of glutaraldehyde prior to the formation of the gel bead, to prevent leakage of the enzyme. Propylene glycol alginate is then added to the mixture. Once the gel beads are formed, they are then soaked in glutaraldehyde, which imparts structural stability to the gel beads. This method can be used with many types of enzymes, such as proteases, carbohydrases, proteases, ligases, isomerases, oxidoreductases, and specialty enzymes. These and other enzymes can be immobilized in the gel beads and utilized in a number of enzymatic processes. Exogenously added ions are not required to maintain the structural stability of these gel beads.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}

Patent:

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