Structurally stable gel bead containing entrapped enzyme and method for manufacture thereof
Abstract
A structurally stable gel bead containing an entrapped enzyme and a method for its manufacture. The enzyme is covalently cross-linked to gelatin in the presence of glutaraldehyde prior to the formation of the gel bead, to prevent leakage of the enzyme. Propylene glycol alginate is then added to the mixture. Once the gel beads are formed, they are then soaked in glutaraldehyde, which imparts structural stability to the gel beads. This method can be used with many types of enzymes, such as proteases, carbohydrases, proteases, ligases, isomerases, oxidoreductases, and specialty enzymes. These and other enzymes can be immobilized in the gel beads and utilized in a number of enzymatic processes. Exogenously added ions are not required to maintain the structural stability of these gel beads.
- Inventors:
-
- Oak Ridge, TN
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- OSTI Identifier:
- 872026
- Patent Number(s):
- 5846762
- Assignee:
- Lockheed Martin Energy Research Systems, Inc. (Oak Ridge, TN)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- AC05-84OR21400
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- structurally; stable; bead; containing; entrapped; enzyme; method; manufacture; covalently; cross-linked; gelatin; presence; glutaraldehyde; prior; formation; prevent; leakage; propylene; glycol; alginate; added; mixture; beads; formed; soaked; imparts; structural; stability; types; enzymes; proteases; carbohydrases; ligases; isomerases; oxidoreductases; specialty; immobilized; utilized; enzymatic; processes; exogenously; required; maintain; propylene glycol; prevent leakage; bead containing; structurally stable; structural stability; containing entrapped; glycol alginate; entrapped enzyme; /435/264/427/428/
Citation Formats
Woodward, Jonathan. Structurally stable gel bead containing entrapped enzyme and method for manufacture thereof. United States: N. p., 1998.
Web.
Woodward, Jonathan. Structurally stable gel bead containing entrapped enzyme and method for manufacture thereof. United States.
Woodward, Jonathan. Thu .
"Structurally stable gel bead containing entrapped enzyme and method for manufacture thereof". United States. https://www.osti.gov/servlets/purl/872026.
@article{osti_872026,
title = {Structurally stable gel bead containing entrapped enzyme and method for manufacture thereof},
author = {Woodward, Jonathan},
abstractNote = {A structurally stable gel bead containing an entrapped enzyme and a method for its manufacture. The enzyme is covalently cross-linked to gelatin in the presence of glutaraldehyde prior to the formation of the gel bead, to prevent leakage of the enzyme. Propylene glycol alginate is then added to the mixture. Once the gel beads are formed, they are then soaked in glutaraldehyde, which imparts structural stability to the gel beads. This method can be used with many types of enzymes, such as proteases, carbohydrases, proteases, ligases, isomerases, oxidoreductases, and specialty enzymes. These and other enzymes can be immobilized in the gel beads and utilized in a number of enzymatic processes. Exogenously added ions are not required to maintain the structural stability of these gel beads.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}
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