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Title: Microfabricated, flowthrough porous apparatus for discrete detection of binding reactions

Abstract

An improved microfabricated apparatus for conducting a multiplicity of individual and simultaneous binding reactions is described. The apparatus comprises a substrate on which are located discrete and isolated sites for binding reactions. The apparatus is characterized by discrete and isolated regions that extend through said substrate and terminate on a second surface thereof such that when a test sample is allowed to the substrate, it is capable of penetrating through each such region during the course of said binding reaction. The apparatus is especially useful for sequencing by hybridization of DNA molecules.

Inventors:
 [1]
  1. The Woodlands, TX
Issue Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
OSTI Identifier:
872010
Patent Number(s):
5843767
Assignee:
Houston Advanced Research Center (Woodlands, TX)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
microfabricated; flowthrough; porous; apparatus; discrete; detection; binding; reactions; improved; conducting; multiplicity; individual; simultaneous; described; comprises; substrate; located; isolated; sites; characterized; regions; extend; terminate; surface; sample; allowed; capable; penetrating; region; course; reaction; especially; useful; sequencing; hybridization; dna; molecules; especially useful; apparatus comprises; dna molecules; dna molecule; apparatus comprise; binding reactions; /435/422/427/428/436/

Citation Formats

Beattie, Kenneth L. Microfabricated, flowthrough porous apparatus for discrete detection of binding reactions. United States: N. p., 1998. Web.
Beattie, Kenneth L. Microfabricated, flowthrough porous apparatus for discrete detection of binding reactions. United States.
Beattie, Kenneth L. Thu . "Microfabricated, flowthrough porous apparatus for discrete detection of binding reactions". United States. https://www.osti.gov/servlets/purl/872010.
@article{osti_872010,
title = {Microfabricated, flowthrough porous apparatus for discrete detection of binding reactions},
author = {Beattie, Kenneth L},
abstractNote = {An improved microfabricated apparatus for conducting a multiplicity of individual and simultaneous binding reactions is described. The apparatus comprises a substrate on which are located discrete and isolated sites for binding reactions. The apparatus is characterized by discrete and isolated regions that extend through said substrate and terminate on a second surface thereof such that when a test sample is allowed to the substrate, it is capable of penetrating through each such region during the course of said binding reaction. The apparatus is especially useful for sequencing by hybridization of DNA molecules.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}

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