Isolation and characterization of Agouti: a diabetes/obesity related gene

Title: Isolation and characterization of Agouti: a diabetes/obesity related gene

Full Record
Other Related Research

Abstract

The present invention relates to the cloning and expression of the Agouti gene and analogous genes in transformed, transfected and transgenic mice. The present invention provides an animal model for the study of diabetes, obesity and tumors for the testing of potential therapeutic agents. The present invention provides oligonucleotide probes for the detection of the Agouti gene and mutations in the gene. The present invention also relates to the isolation and recombinant production of the Agouti gene product, production of antibodies to the Agouti gene product and their use as diagnostic and therapeutic agents.

Inventors:

Woychik, Richard P. ^[1]

(Knoxville, TN)

Issue Date:: 1998-01-01

Research Org.:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN

OSTI Identifier:: 872007

Patent Number(s):: 5843652

Assignee:: Lockheed Martin Energy Systems, Inc. (Oak Ridge, TN) ORNL

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: isolation; characterization; agouti; diabetes; obesity; related; relates; cloning; expression; analogous; genes; transformed; transfected; transgenic; mice; provides; animal; model; study; tumors; testing; potential; therapeutic; agents; oligonucleotide; probes; detection; mutations; recombinant; production; product; antibodies; diagnostic; oligonucleotide probes; therapeutic agents; recombinant production; provides oligonucleotide; transgenic mice; therapeutic agent; animal model; analogous genes; potential therapeutic; obesity related; oligonucleotide probe; /435/999/

Citation Formats


                    Woychik, Richard P. Isolation and characterization of Agouti: a diabetes/obesity related gene.  United States: N. p., 1998. 
        Web.

Copy to clipboard


                    Woychik, Richard P. Isolation and characterization of Agouti: a diabetes/obesity related gene.  United States.

Copy to clipboard


                    Woychik, Richard P. Thu .  
        "Isolation and characterization of Agouti: a diabetes/obesity related gene".  United States.  https://www.osti.gov/servlets/purl/872007.

Copy to clipboard


                    
@article{osti_872007,

  title        = {Isolation and characterization of Agouti: a diabetes/obesity related gene},

  author       = {Woychik, Richard P.},

  abstractNote = {The present invention relates to the cloning and expression of the Agouti gene and analogous genes in transformed, transfected and transgenic mice. The present invention provides an animal model for the study of diabetes, obesity and tumors for the testing of potential therapeutic agents. The present invention provides oligonucleotide probes for the detection of the Agouti gene and mutations in the gene. The present invention also relates to the isolation and recombinant production of the Agouti gene product, production of antibodies to the Agouti gene product and their use as diagnostic and therapeutic agents.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1998},

  month        = {1}

}

Copy to clipboard

Patent:

View Patent

Save / Share:

Export Metadata

Save to My Library

Similar records in DOE Patents and OSTI.GOV collections:

Isolation and characterization of Agouti: a diabetes/obesity related gene

Patent Woychik, Richard P. (Knoxville, TN)

The present invention relates to the cloning and expression of the Agouti gene and analogous genes in transformed, transfected and transgenic mice. The present invention provides an animal model for the study of diabetes, obesity and tumors for the testing of potential therapeutic agents. The present invention provides oligonucleotide probes for the detection of the Agouti gene and mutations in the gene. The present invention also relates to the isolation and recombinant production of the Agouti gene product, production of antibodies to the Agouti gene product and their use as diagnostic and therapeutic agents.
Full Text Available
Isolation and characterization of Agouti: a diabetes/obesity related gene

Patent Woychik, Richard P. (Knoxville, TN)

The present invention relates to the cloning and expression of the Agouti gene and analogous genes in transformed, transfected and transgenic mice. The present invention provides an animal model for the study of diabetes, obesity and tumors for the testing of potential therapeutic agents. The present invention provides oligonucleotide probes for the detection of the Agouti gene and mutations in the gene. The present invention also relates to the isolation and recombinant production of the Agouti gene product, production of antibodies to the Agouti gene product and their use as diagnostic and therapeutic agents.
Full Text Available
Ectopic expression of the agouti gene in transgenic mice causes obesity, features of type II diabetes, and yellow fur

Journal Article Klebig, M.L.; Woychik, R.P. [Oak Ridge National Laboratory, Oak Ridge, TN (United States)]; Wilkinson, J.E. [Univ. of Tennessee, Knoxville, TN (United States)]; ... - Proceedings of the National Academy of Sciences of the United States of America

Mice that carry the lethal yellow (A{sup y}) or viable yellow (A{sup vy}) mutation, two dominant mutations of the agouti (a) gene in mouse chromosome 2, exhibit a phenotype that includes yellow fur, marked obesity, a form of type II diabetes associated with insulin resistance, and an increased susceptibility to tumor development. Molecular analyses of these and several other dominant {open_quotes}obese yellow{close_quotes} a-locus mutations suggested that ectopic expression of the normal agouti protein gives rise to this complex pleiotropic phenotype. We have now tested this hypothesis directly by generating transgenic mice that ectopically express an agouti cDNA clone encoding themore »« less
DOI: 10.1073/pnas.92.11.4728
Analysis of the function of the agouti gene in obesity and diabetes

Conference Mynatt, R.L.; Miltenberger, R.J.; Klebig, M.L. [and others]

This chapter discusses the agouti gene and dominant mutations in that gene that lead to agouti-induced obesity, and recent work with transgenic mice to elucidate the role of agouti in obesity. Agouti was cloned in 1992 by the lab of Rick Woychik at Oak Ridge National Laboratory, making it the first of many recently cloned mouse obesity genes. Sequence analysis predicted that mouse agouti is a secreted protein of 131 amino acids. The mature protein has a basic central region (lys57-arg85), a proline-rich domain (pro86-pro91) and a C-terminal region (cys 92-cys 13 1) containing 10 cysteine residues which form 5more »« less
Full Text Available
Liver-specific expression of the agouti gene in transgenic mice promotes liver carcinogenesis in the absence of obesity and diabetes

Journal Article Kuklin, Alexander [ORNL]; Mynatt, Randall [ORNL]; Klebig, Mitch [ORNL]; ... - Molecular Cancer

Background: The agouti protein is a paracrine factor that is normally present in the skin of many species of mammals. Agouti regulates the switch between black and yellow hair pigmentation by signalling through the melanocortin 1 receptor (Mc1r) on melanocytes. Lethal yellow (Ay) and viable yellow (Avy) are dominant regulatory mutations in the mouse agouti gene that cause the wild- ype protein to be produced at abnormally high levels throughout the body. Mice harboring these mutations exhibit a pleiotropic syndrome characterized by yellow coat color, obesity, hyperglycemia, hyperinsulinemia, and increased susceptibility to hyperplasia and carcinogenesis in numerous tissues, including themore »« less

Similar Records