Isolation and characterization of Agouti: a diabetes/obesity related gene

Title: Isolation and characterization of Agouti: a diabetes/obesity related gene

Abstract

The present invention relates to the cloning and expression of the Agouti gene and analogous genes in transformed, transfected and transgenic mice. The present invention provides an animal model for the study of diabetes, obesity and tumors for the testing of potential therapeutic agents. The present invention provides oligonucleotide probes for the detection of the Agouti gene and mutations in the gene. The present invention also relates to the isolation and recombinant production of the Agouti gene product, production of antibodies to the Agouti gene product and their use as diagnostic and therapeutic agents.

Inventors:

Woychik, Richard P ^[1]

Knoxville, TN

Issue Date:: 1998-01-01

Research Org.:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

OSTI Identifier:: 872007

Patent Number(s):: 5843652

Assignee:: Lockheed Martin Energy Systems, Inc. (Oak Ridge, TN)

Patent Classifications (CPCs):: A - HUMAN NECESSITIES A01 - AGRICULTURE A01K - ANIMAL HUSBANDRY

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

Show more

A - HUMAN NECESSITIES A01 - AGRICULTURE A01K - ANIMAL HUSBANDRY
A01K2227/105 - Murine
A01K2217/05 - Animals comprising random inserted nucleic acids

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/8509 - {for producing genetically modified animals, e.g. transgenic}

A - HUMAN NECESSITIES A01 - AGRICULTURE A01K - ANIMAL HUSBANDRY
A01K67/0275 - {Genetically modified vertebrates, e.g. transgenic}

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N2830/85 - mammalian

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C07K14/47 - from mammals

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N2830/008 - cell type or tissue specific enhancer/promoter combination

A - HUMAN NECESSITIES A01 - AGRICULTURE A01K - ANIMAL HUSBANDRY
A01K2267/0306 - Animal model for genetic diseases
A01K2267/03 - Animal model, e.g. for test or diseases
A01K2267/0362 - Animal model for lipid/glucose metabolism, e.g. obesity, type-2 diabetes

Show less

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: isolation; characterization; agouti; diabetes; obesity; related; relates; cloning; expression; analogous; genes; transformed; transfected; transgenic; mice; provides; animal; model; study; tumors; testing; potential; therapeutic; agents; oligonucleotide; probes; detection; mutations; recombinant; production; product; antibodies; diagnostic; oligonucleotide probes; therapeutic agents; recombinant production; provides oligonucleotide; transgenic mice; therapeutic agent; animal model; analogous genes; potential therapeutic; obesity related; oligonucleotide probe; /435/999/

Citation Formats


                    Woychik, Richard P. Isolation and characterization of Agouti: a diabetes/obesity related gene.  United States: N. p., 1998. 
        Web.

Copy to clipboard


                    Woychik, Richard P. Isolation and characterization of Agouti: a diabetes/obesity related gene.  United States.

Copy to clipboard


                    Woychik, Richard P. Thu .  
        "Isolation and characterization of Agouti: a diabetes/obesity related gene".  United States.  https://www.osti.gov/servlets/purl/872007.

Copy to clipboard


                    
@article{osti_872007,

  title        = {Isolation and characterization of Agouti: a diabetes/obesity related gene},

  author       = {Woychik, Richard P},

  abstractNote = {The present invention relates to the cloning and expression of the Agouti gene and analogous genes in transformed, transfected and transgenic mice. The present invention provides an animal model for the study of diabetes, obesity and tumors for the testing of potential therapeutic agents. The present invention provides oligonucleotide probes for the detection of the Agouti gene and mutations in the gene. The present invention also relates to the isolation and recombinant production of the Agouti gene product, production of antibodies to the Agouti gene product and their use as diagnostic and therapeutic agents.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1998},

  month        = {1}

}

Copy to clipboard

Patent:

View Patent

Save / Share:

Export Metadata

Save to My Library

Works referenced in this record:

Molecular structure and chromosomal mapping of the human homolog of the agouti gene.
journal, October 1994

Kwon, H. Y.; Bultman, S. J.; Loffler, C.
Proceedings of the National Academy of Sciences, Vol. 91, Issue 21
https://doi.org/10.1073/pnas.91.21.9760

Detection of human apolipoprotein E3, E2, and E4 genotypes by an allele-specific oligonucleotide-primed polymerase chain reaction assay: development and validation
journal, July 1991

Green, E. K.; Bain, S. C.; Day, P. J.
Clinical Chemistry, Vol. 37, Issue 7
https://doi.org/10.1093/clinchem/37.7.1263

Similar Records in DOE Patents and OSTI.GOV collections:

Analysis of the function of the agouti gene in obesity and diabetes

Conference Mynatt, R L; Miltenberger, R J; Klebig, M L [and others]

This chapter discusses the agouti gene and dominant mutations in that gene that lead to agouti-induced obesity, and recent work with transgenic mice to elucidate the role of agouti in obesity. Agouti was cloned in 1992 by the lab of Rick Woychik at Oak Ridge National Laboratory, making it the first of many recently cloned mouse obesity genes. Sequence analysis predicted that mouse agouti is a secreted protein of 131 amino acids. The mature protein has a basic central region (lys57-arg85), a proline-rich domain (pro86-pro91) and a C-terminal region (cys 92-cys 13 1) containing 10 cysteine residues which form 5more »« less
Full Text Available
Liver-specific expression of the agouti gene in transgenic mice promotes liver carcinogenesis in the absence of obesity and diabetes

Journal Article Kuklin, Alexander [ORNL]; Mynatt, Randall [ORNL]; Klebig, Mitch [ORNL]; ... - Molecular Cancer

Background: The agouti protein is a paracrine factor that is normally present in the skin of many species of mammals. Agouti regulates the switch between black and yellow hair pigmentation by signalling through the melanocortin 1 receptor (Mc1r) on melanocytes. Lethal yellow (Ay) and viable yellow (Avy) are dominant regulatory mutations in the mouse agouti gene that cause the wild- ype protein to be produced at abnormally high levels throughout the body. Mice harboring these mutations exhibit a pleiotropic syndrome characterized by yellow coat color, obesity, hyperglycemia, hyperinsulinemia, and increased susceptibility to hyperplasia and carcinogenesis in numerous tissues, including themore »« less
Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus

Patent Dai, Ziyu; Lasure, Linda L; Magnuson, Jon K

The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression ofmore »« less
Full Text Available
Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus

Patent Dai, Ziyu [Richland, WA]; Lasure, Linda L [Fall City, WA]; Magnuson, Jon K [Pasco, WA]

The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression ofmore »« less
Full Text Available
Method to produce acetyldiacylglycerols (ac-TAGs) by expression of an acetyltransferase gene isolated from Euonymus alatus (burning bush)

Patent Durrett, Timothy; Ohlrogge, John; Pollard, Michael

The present invention relates to novel diacylglycerol acyltransferase genes and proteins, and methods of their use. In particular, the invention describes genes encoding proteins having diacylglycerol acetyltransferase activity, specifically for transferring an acetyl group to a diacylglycerol substrate to form acetyl-Triacylglycerols (ac-TAGS), for example, a 3-acetyl-1,2-diacyl-sn-glycerol. The present invention encompasses both native and recombinant wild-type forms of the transferase, as well as mutants and variant forms. The present invention also relates to methods of using novel diacylglycerol acyltransferase genes and proteins, including their expression in transgenic organisms at commercially viable levels, for increasing production of 3-acetyl-1,2-diacyl-sn-glycerols in plant oils andmore »« less
Full Text Available

Similar Records

Title: Isolation and characterization of Agouti: a diabetes/obesity related gene

Abstract

Citation Formats

Molecular structure and chromosomal mapping of the human homolog of the agouti gene. journal, October 1994

Detection of human apolipoprotein E3, E2, and E4 genotypes by an allele-specific oligonucleotide-primed polymerase chain reaction assay: development and validation journal, July 1991

Molecular structure and chromosomal mapping of the human homolog of the agouti gene.
journal, October 1994

Detection of human apolipoprotein E3, E2, and E4 genotypes by an allele-specific oligonucleotide-primed polymerase chain reaction assay: development and validation
journal, July 1991